Mouse Polyclonal ARHGEF10 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF10 aa 200-600.
Images
Key facts
- Isotype
IgG
- Host species
Mouse
- Storage buffer
pH: 7.4
Constituents: 99% PBS- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARHGEF10 aa 200-600. The exact immunogen used to generate this antibody is proprietary information. Database link O15013
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Target data
Function
May play a role in developmental myelination of peripheral nerves.
Alternative names
Rho guanine nucleotide exchange factor 10, ARHGEF10, KIAA0294
Recommended products
Mouse Polyclonal ARHGEF10 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF10 aa 200-600.
Alternative names
Rho guanine nucleotide exchange factor 10, ARHGEF10, KIAA0294
Key facts
- Isotype
IgG
- Host species
Mouse
- Storage buffer
pH: 7.4
Constituents: 99% PBS- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARHGEF10 aa 200-600. The exact immunogen used to generate this antibody is proprietary information. Database link O15013
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARHGEF10 also known as Rho guanine nucleotide exchange factor 10 functions as a guanine nucleotide exchange factor initiating the activation of Rho GTPases by facilitating the exchange of GDP for GTP. This protein has a mass of approximately 85 kDa. It shows a broad expression pattern with significant presence in nervous tissues particularly within the peripheral nervous system. Its presence in these regions highlights its potential role in neural development and signaling.
- Biological function summary
ARHGEF10 plays a part in cell morphogenesis and migration by impacting the Rho signaling pathway. It associates within complexes that modulate cytoskeletal dynamics. Its influence is especially critical in Schwann cells where it contributes to the organization and maintenance of the myelin sheath an essential feature for proper nerve conduction. These biological roles suggest ARHGEF10’s importance in cellular architecture and response to extracellular signals.
- Pathways
ARHGEF10 integrates within the Rho GTPase pathway impacting actin cytoskeleton organization and cell motility. It interacts with proteins such as RHOA and RHOB guiding cellular responses to extracellular stimuli. Additionally it partakes in the myelination pathway affecting the structural alignment and integrity of neural tissues. These interactions help refine our understanding of its involvement in regulating cellular dynamics.
- Associated diseases and disorders
ARHGEF10 holds a clinical connection to Charcot-Marie-Tooth disease type 4H a peripheral neuropathy characterized by demyelination and sensory loss. Its role emerges through its interaction with neural proteins that maintain myelin integrity. Moreover it shows associations with diseases involving nerve regeneration impairments potentially involving proteins like PMP22 in these pathological contexts. These associations spotlight ARHGEF10 as a significant actor in neuropathy studies offering potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ARHGEF10 antibody (ab168936)
All lanes: Western blot - Anti-ARHGEF10 antibody (ab168936) at 1 µg/mL
Lane 1: ARHGEF10-transfected 293T cell lysate (41.8 KDa) at 15 µL
Lane 2: non-transfected 293T cell lysate at 15 µL
Developed using the ECL technique.
Predicted band size: 116 kDa, 152 kDa, 41 kDa, 49 kDa, 62 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ARHGEF10 antibody (ab168936)
Immunofluorescent analysis of HeLa cells, labeling ARHGEF10 with ab168936 at 10 µg/ml.
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