Rabbit Polyclonal ARHGEF5 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF5 aa 1450 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARHGEF5 aa 1450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q12774
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Guanine nucleotide exchange factor which activates Rho GTPases (PubMed:15601624). Strongly activates RHOA (PubMed:15601624). Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (By similarity). Involved in regulation of cell shape and actin cytoskeletal organization (PubMed:15601624). Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (PubMed:14662653). Required for SRC-induced podosome formation (By similarity). Involved in positive regulation of immature dendritic cell migration (By similarity).
Alternative names
TIM, ARHGEF5, Rho guanine nucleotide exchange factor 5, Ephexin-3, Guanine nucleotide regulatory protein TIM, Oncogene TIM, Transforming immortalized mammary oncogene, p60 TIM
Recommended products
Rabbit Polyclonal ARHGEF5 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF5 aa 1450 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARHGEF5 aa 1450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q12774
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARHGEF5 also known as Rho guanine nucleotide exchange factor 5 is a critical protein involved in the regulation of Rho GTPases. This protein acts mechanically as a catalyst exchanging GDP for GTP on Rho GTPases which are powerful regulators of the actin cytoskeleton and cell morphology. ARHGEF5 has an approximate molecular mass of 140 kDa and it is expressed in a variety of tissues including the brain heart and lungs among others. In these locations ARHGEF5 plays an essential role in enabling cellular functions that depend on cytoskeletal dynamics.
- Biological function summary
ARHGEF5 actively contributes to cellular processes by modulating actin cytoskeleton organization and cell migration. It typically functions as part of signaling complexes that facilitate cellular communication and response to extracellular signals. This modulation is important for processes like cell adhesion movement and growth which are necessary for normal cellular operations and reactions to environmental changes.
- Pathways
ARHGEF5 plays an integral role in the Rho signaling pathway and the focal adhesion pathway. These pathways control various cellular processes including cytoskeleton reorganization and cell adhesion both of which are essential for maintaining proper cellular structure and function. Within these pathways ARHGEF5 interacts with other key proteins such as RhoA and Rac1 amplifying its effect on cellular morphology and movement.
- Associated diseases and disorders
ARHGEF5 is associated with cancer and neurological disorders. Aberrant expression or function of ARHGEF5 has been implicated in the progress of different cancer types due to its role in cell migration and invasion. In neurological disorders ARHGEF5 may affect neuronal migration and connectivity. Additionally through its interactions with proteins like PI3K ARHGEF5 may influence cancer cell survival and proliferation further implicating its role in oncogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGEF5 antibody (ab244433)
Paraffin-embedded human duodenum tissue stained for ARHGEF5 using ab244433 at 1/200 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-ARHGEF5 antibody (ab244433)
PFA-fixed, Triton X-100 permeabilized SiHa cells stained for ARHGEF5 (green) using ab244433 at 4 μg/ml in ICC/IF.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com