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AB244433

Anti-ARHGEF5 antibody

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(1 Publication)

Rabbit Polyclonal ARHGEF5 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF5 aa 1450 to C-terminus.

View Alternative Names

TIM, ARHGEF5, Rho guanine nucleotide exchange factor 5, Ephexin-3, Guanine nucleotide regulatory protein TIM, Oncogene TIM, Transforming immortalized mammary oncogene, p60 TIM

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ARHGEF5 antibody (AB244433)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ARHGEF5 antibody (AB244433)

PFA-fixed, Triton X-100 permeabilized SiHa cells stained for ARHGEF5 (green) using ab244433 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGEF5 antibody (AB244433)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGEF5 antibody (AB244433)

Paraffin-embedded human duodenum tissue stained for ARHGEF5 using ab244433 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ARHGEF5 aa 1450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q12774

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARHGEF5 also known as Rho guanine nucleotide exchange factor 5 is a critical protein involved in the regulation of Rho GTPases. This protein acts mechanically as a catalyst exchanging GDP for GTP on Rho GTPases which are powerful regulators of the actin cytoskeleton and cell morphology. ARHGEF5 has an approximate molecular mass of 140 kDa and it is expressed in a variety of tissues including the brain heart and lungs among others. In these locations ARHGEF5 plays an essential role in enabling cellular functions that depend on cytoskeletal dynamics.
Biological function summary

ARHGEF5 actively contributes to cellular processes by modulating actin cytoskeleton organization and cell migration. It typically functions as part of signaling complexes that facilitate cellular communication and response to extracellular signals. This modulation is important for processes like cell adhesion movement and growth which are necessary for normal cellular operations and reactions to environmental changes.

Pathways

ARHGEF5 plays an integral role in the Rho signaling pathway and the focal adhesion pathway. These pathways control various cellular processes including cytoskeleton reorganization and cell adhesion both of which are essential for maintaining proper cellular structure and function. Within these pathways ARHGEF5 interacts with other key proteins such as RhoA and Rac1 amplifying its effect on cellular morphology and movement.

ARHGEF5 is associated with cancer and neurological disorders. Aberrant expression or function of ARHGEF5 has been implicated in the progress of different cancer types due to its role in cell migration and invasion. In neurological disorders ARHGEF5 may affect neuronal migration and connectivity. Additionally through its interactions with proteins like PI3K ARHGEF5 may influence cancer cell survival and proliferation further implicating its role in oncogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide exchange factor which activates Rho GTPases (PubMed : 15601624). Strongly activates RHOA (PubMed : 15601624). Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (By similarity). Involved in regulation of cell shape and actin cytoskeletal organization (PubMed : 15601624). Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (PubMed : 14662653). Required for SRC-induced podosome formation (By similarity). Involved in positive regulation of immature dendritic cell migration (By similarity).
See full target information ARHGEF5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Cell physiology 319:C93-C104 PubMed31747529

2019

Downregulation of AAA-domain-containing protein 2 restrains cancer stem cell properties in esophageal squamous cell carcinoma via blockade of the Hedgehog signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nuo Li,Yang Yu,Baoming Wang
View all publications
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Product promise

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