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AB236103

Anti-ARHGEF7 antibody

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(1 Publication)

Rabbit Polyclonal ARHGEF7 antibody. Suitable for IP, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF7 aa 1-250.

View Alternative Names

COOL1, KIAA0142, P85SPR, PAK3BP, PIXB, Nbla10314, ARHGEF7, Rho guanine nucleotide exchange factor 7, Beta-Pix, COOL-1, PAK-interacting exchange factor beta, p85

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-ARHGEF7 antibody (AB236103)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARHGEF7 antibody (AB236103)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for ARHGEF7 (Green) using ab236103 at 1/250 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI (Blue).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IP

applications

Immunogen

Recombinant Fragment Protein within Human ARHGEF7 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q14155

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARHGEF7 also known as beta-PIX or Cool-1 is a guanine nucleotide exchange factor (GEF) involved in activating Rho GTPases specifically Rac1 and Cdc42. The protein plays a critical role in signal transduction by facilitating the exchange of GDP for GTP on these small GTPases leading to changes in cell morphology and motility. ARHGEF7 has a molecular mass of approximately 82 kDa and is widely expressed in various tissues including the brain and lungs. It commonly finds expression in neurons and plays a significant role in cytoskeletal dynamics.
Biological function summary

ARHGEF7 contributes to cell adhesion migration and synaptic plasticity. It interacts with other cytoskeletal-associated proteins forming complexes that regulate actin cytoskeleton organization. In neuronal cells ARHGEF7 is essential for dendritic spine formation and synapse maintenance facilitating neurotransmission and neural connectivity. Through its role in modulating actin dynamics ARHGEF7 influences cellular protrusions essential for communication between cells.

Pathways

ARHGEF7 plays a significant role in pathways controlling cell migration and signaling. It interacts within the Rac1 and Cdc42 pathways pivotal in reorganizing the actin cytoskeleton and forming cellular protrusions such as filopodia and lamellipodia. ARHGEF7 links functionally with proteins like PAK kinases that modulate cell movement and signaling pathways. These pathways collectively contribute to cellular communication and tissue structuring.

ARHGEF7 connections exist with neurological disorders such as intellectual disability and autism spectrum disorders. Dysregulation of ARHGEF7 disrupts synaptic function and neural architecture which can result in cognitive and developmental deficits. Additionally ARHGEF7 interacts with proteins like DISC1 influencing psychiatric conditions. The precise role of ARHGEF7 in these disorders requires further investigation to fully understand its therapeutic implications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.
See full target information ARHGEF7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 29:e70388 PubMed39988987

2025

Baicalin Prevents Chronic β-AR Agonist-Induced Heart Failure via Preventing Oxidative Stress and Overactivation of the NADPH Oxidase NOX2.

Applications

Unspecified application

Species

Unspecified reactive species

Yixuan Ge,En Ma,Xiaowei Guo,Qing Wang,Weidong Zhu,Dan-Ni Ren,Da Wo
View all publications
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Product promise

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