AB272905

Anti-ARID1A antibody

Name: Anti-ARID1A antibody (ab272905) | Abcam
Brand: Abcam Limited
SKU: AB272905
Price: 530 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal ARID1A antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARID1A.

View Alternative Names

BAF250, BAF250A, C1orf4, OSA1, SMARCF1, ARID1A, AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A, B120, BRG1-associated factor 250, BRG1-associated factor 250a, Osa homolog 1, SWI-like protein, SWI/SNF complex protein p270, hELD, hOSA1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody (AB272905)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody (AB272905)

Paraffin embedded human cervical carcinoma tissue stained for ARID1A using ab272905 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody (AB272905)

Paraffin embedded human endometrial carcinoma tissue stained for ARID1A using ab272905 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-ARID1A antibody (AB272905)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARID1A antibody (AB272905)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling ARID1A protein at nucleus using ab272905 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red).

Scale bar = 10 µm

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Immunoprecipitation - Anti-ARID1A antibody (AB272905)

HepG2 whole cell extract immunoprecipitation analysis.

A : Control with 4 μg of preimmune Rabbit IgG
B : Immunoprecipitation of ARID1A protein with 4 μg of ab272905.

5 % SDS-PAGE. The immunoprecipitated ARID1A protein was detected using ab272905 at 1/500 dilution.

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Immunoprecipitation - Anti-ARID1A antibody (ab272905)

Predicted band size: 242 kDa

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Western blot - Anti-ARID1A antibody (AB272905)

All lanes:

Western blot - Anti-ARID1A antibody (ab272905) at 1/1000 dilution

All lanes:

A549 cell lysate at 30 µg

Predicted band size: 242 kDa

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Western blot - Anti-ARID1A antibody (AB272905)

All lanes:

Western blot - Anti-ARID1A antibody (ab272905) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

HepG2 nuclear extract cell lysate at 30 µg

Predicted band size: 242 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P, IP

applications

Immunogen

Recombinant Fragment Protein within Human ARID1A. The exact immunogen used to generate this antibody is proprietary information.

O14497

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 2.42% Tris, 1.5% Glycine, 0.04% EGTA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARID1A also known as BAF250A is a protein encoded by the ARID1A gene and forms part of the SWI/SNF chromatin remodeling complex. The molecular weight of ARID1A is approximately 270 kDa. This protein is expressed in many tissues but higher expression is noted in the liver kidney and placenta. ARID1A contains an ARID (A-T Rich Interaction Domain) which allows it to bind DNA influencing transcription regulation significantly.

Biological function summary

ARID1A impacts cellular functions through its role in the SWI/SNF complex. This complex modulates chromatin structure and is integral to DNA accessibility which in turn controls gene expression. ARID1A's involvement helps in regulating transcription factors and affects processes such as cell growth and differentiation. Its function is necessary for maintaining proper cell proliferation and apoptosis balance.

Pathways

ARID1A plays a central role in transcriptional regulation and cell cycle control. It shows interaction with the tumor suppressor protein p53 and is implicated in the PI3K/AKT signaling pathway. ARID1A's function in these pathways affects cellular responses to DNA damage and growth signals linking it closely to important cellular fate decisions.

Mutations or loss of function in ARID1A has been strongly associated with various cancers including ovarian and endometrial cancers. In these contexts ARID1A dysfunction often occurs alongside other proteins such as p53 contributing to tumorigenesis by deregulating tumor suppressive pathways. It also holds a potential connection to neurodevelopmental disorders due to its role in chromatin remodeling and gene expression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).

See full target information ARID1A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Research (Washington, D.C.) 8:0908 PubMed41049615

2025

ARID1A Governs Genomic Stability and Proliferation in SCLC via c-MYC/PARP1 Suppression Driving Vulnerability to BET Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Guozhen Cao,Liying Ma, Xueqin Dai,Peng Hou,Xinhuang Yao,Gongfeng Li,Jiahui Zhang,Ceshi Chen,Wenchu Lin

Nature 646:676-686 PubMed40770097

2025

Microglia regulate GABAergic neurogenesis in prenatal human brain through IGF1.

Applications

Unspecified application

Species

Unspecified reactive species

Diankun Yu,Samhita Jain,Andi Wangzhou,Beika Zhu,Wenyuan Shao,Elena J Coley-O'Rourke,Stacy De Florencio,JaeYeon Kim,Jennifer Ja-Yoon Choi,Mercedes F Paredes,Tomasz J Nowakowski,Eric J Huang,Xianhua Piao

Cancers 15: PubMed37173914

2023

Chromatin Remodelling Molecule ARID1A Determines Metastatic Heterogeneity in Triple-Negative Breast Cancer by Competitively Binding to YAP.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Wang,Xinyu Chen,Xiaosu Qiao,Yizhao Xie,Duancheng Guo,Bin Li,Jianing Cao,Zhonghua Tao,Xichun Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ARID1A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody (AB272905)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody (AB272905)

Immunocytochemistry/ Immunofluorescence - Anti-ARID1A antibody (AB272905)

Immunoprecipitation - Anti-ARID1A antibody (AB272905)

Western blot - Anti-ARID1A antibody (AB272905)

Western blot - Anti-ARID1A antibody (AB272905)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise