AB242377

Anti-ARID1A antibody [CL3595]

Name: Anti-ARID1A antibody [CL3595] (ab242377) | Abcam
Brand: Abcam Limited
SKU: AB242377
Price: 630 USD
Availability: InStock

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(6 Publications)

Mouse Monoclonal ARID1A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARID1A aa 1250-1400.

View Alternative Names

BAF250, BAF250A, C1orf4, OSA1, SMARCF1, ARID1A, AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A, B120, BRG1-associated factor 250, BRG1-associated factor 250a, Osa homolog 1, SWI-like protein, SWI/SNF complex protein p270, hELD, hOSA1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (AB242377)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (AB242377)

Paraffin-embedded human fallopian tube tissue stained for ARID1A with ab242377 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (AB242377)

Paraffin-embedded human breast cancer tissue stained for ARID1A with ab242377 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (AB242377)

Paraffin-embedded human liver cancer tissue stained for ARID1A with ab242377 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (AB242377)

Paraffin-embedded human prostate cancer tissue stained for ARID1A with ab242377 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (AB242377)

Paraffin-embedded human colon tissue stained for ARID1A with ab242377 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [CL3595] (AB242377)

Paraffin-embedded human kidney tissue stained for ARID1A with ab242377 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ARID1A antibody [CL3595] (AB242377)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARID1A antibody [CL3595] (AB242377)

PFA-fixed, Triton X-100 permeabilized A431 cells stained for ARID1A (Green) using ab242377 at 4 μg/ml in ICC/IF.

Microtublues are stained red.

Supplier Data

Western blot - Anti-ARID1A antibody [CL3595] (AB242377)

All lanes:

Western blot - Anti-ARID1A antibody [CL3595] (ab242377)

Lane 1:

Empty vector-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

ARID1A over-expression (co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK-293T), cell lysate

Predicted band size: 242 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3595

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ARID1A aa 1250-1400. The exact immunogen used to generate this antibody is proprietary information.

O14497

Epitope

Binds to an epitope located within the peptide sequence VAMGPRQHYPYGGPY as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

purified from TCS.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARID1A also known as BAF250A is a protein encoded by the ARID1A gene and forms part of the SWI/SNF chromatin remodeling complex. The molecular weight of ARID1A is approximately 270 kDa. This protein is expressed in many tissues but higher expression is noted in the liver kidney and placenta. ARID1A contains an ARID (A-T Rich Interaction Domain) which allows it to bind DNA influencing transcription regulation significantly.

Biological function summary

ARID1A impacts cellular functions through its role in the SWI/SNF complex. This complex modulates chromatin structure and is integral to DNA accessibility which in turn controls gene expression. ARID1A's involvement helps in regulating transcription factors and affects processes such as cell growth and differentiation. Its function is necessary for maintaining proper cell proliferation and apoptosis balance.

Pathways

ARID1A plays a central role in transcriptional regulation and cell cycle control. It shows interaction with the tumor suppressor protein p53 and is implicated in the PI3K/AKT signaling pathway. ARID1A's function in these pathways affects cellular responses to DNA damage and growth signals linking it closely to important cellular fate decisions.

Mutations or loss of function in ARID1A has been strongly associated with various cancers including ovarian and endometrial cancers. In these contexts ARID1A dysfunction often occurs alongside other proteins such as p53 contributing to tumorigenesis by deregulating tumor suppressive pathways. It also holds a potential connection to neurodevelopmental disorders due to its role in chromatin remodeling and gene expression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).

See full target information ARID1A

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Food science and biotechnology 34:743-755 PubMed39958166

2025

Long-term Pu-erh tea alleviates inflammatory bowel disease via the regulation of intestinal microbiota and maintaining the intestinal mucosal barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Bi-Yun Feng,Pei-Lu Chen,Ling Yan,Wei-Feng Huang,Cheng-Fu Li,Li-Tao Yi,Guang-Hui Xu

Frontiers in bioscience (Landmark edition) 26:1411-1421 PubMed34994156

2022

ARID1A-mutated cervical cancer depends on the activation of YAP signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Gao,Peiling Li,Xianchao Kong,Tiefang Song,Qi Han,Suiyang Zhou

The Kaohsiung journal of medical sciences 37:940-950 PubMed34414664

2021

LncRNA GAS5 inhibits the proliferation and invasion of ovarian clear cell carcinoma via the miR-31-5p/ARID1A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhang,Zhong-Mei Yang,Yu Huang,Ka-Na Wang,Yao Xie,Nian Yang

American journal of cancer research 11:2911-2927 PubMed34249435

2021

Glutamate-cysteine ligase catalytic subunit as a therapeutic target in acute myeloid leukemia and solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Chiou-Hong Lin,John P Vu,Chen-Yen Yang,Mint Sirisawad,Chun-Te Chen,Hung Dao,Jing Liu,Xuan Ma,Chin Pan,Joseph Cefalu,Chris Tse,Erica Jackson,Hsu-Ping Kuo

International journal of molecular medicine 46:1683-1694 PubMed33000179

2020

Role of ARID1A in epithelial‑mesenchymal transition in breast cancer and its effect on cell sensitivity to 5‑FU.

Applications

Unspecified application

Species

Unspecified reactive species

Tangshun Wang,Xiang Gao,Kexin Zhou,Tao Jiang,Shuang Gao,Pengzhou Liu,Ximeng Zuo,Xiaoguang Shi

Acta biochimica et biophysica Sinica 52:150-159 PubMed31912865

2020

miR-223-3p promotes cell proliferation and invasion by targeting Arid1a in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yiping Zhu,Kai Li,Liang Yan,Yang He,Lu Wang,Lili Sheng

View all publications

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