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Rabbit Recombinant Monoclonal ARID1A antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (AB250617), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (AB250617), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (AB250617), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (AB250617), expandable thumbnail
  • ChIC/CUT&RUN sequencing - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (AB250617), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIC/CUT&RUN-seqWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

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      Target data

      Function

      Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).

      Alternative names

      AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A, B120, BRG1-associated factor 250, BRG1-associated factor 250a, Osa homolog 1, SWI-like protein, SWI/SNF complex protein p270, hELD, BAF250, BAF250A, hOSA1, SMARCF1, ARID1A, BAF250, BAF250A, C1orf4, OSA1

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      Rabbit Recombinant Monoclonal ARID1A antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

      Alternative names

      AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A, B120, BRG1-associated factor 250, BRG1-associated factor 250a, Osa homolog 1, SWI-like protein, SWI/SNF complex protein p270, hELD, BAF250, BAF250A, hOSA1, SMARCF1, ARID1A, BAF250, BAF250A, C1orf4, OSA1

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Carrier free

      Yes

      Clone number

      EPR13501-73

      Purification technique

      Affinity purification Protein A

      Concentration
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      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      +4°C

      Storage information

      Do Not Freeze

      Notes

      ab250617 is the carrier-free version of Anti-ARID1A antibody [EPR13501-73] ab182561.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      Supplementary info

      Activity summary

      ARID1A also known as BAF250A is a protein encoded by the ARID1A gene and forms part of the SWI/SNF chromatin remodeling complex. The molecular weight of ARID1A is approximately 270 kDa. This protein is expressed in many tissues but higher expression is noted in the liver kidney and placenta. ARID1A contains an ARID (A-T Rich Interaction Domain) which allows it to bind DNA influencing transcription regulation significantly.

      Biological function summary

      ARID1A impacts cellular functions through its role in the SWI/SNF complex. This complex modulates chromatin structure and is integral to DNA accessibility which in turn controls gene expression. ARID1A's involvement helps in regulating transcription factors and affects processes such as cell growth and differentiation. Its function is necessary for maintaining proper cell proliferation and apoptosis balance.

      Pathways

      ARID1A plays a central role in transcriptional regulation and cell cycle control. It shows interaction with the tumor suppressor protein p53 and is implicated in the PI3K/AKT signaling pathway. ARID1A's function in these pathways affects cellular responses to DNA damage and growth signals linking it closely to important cellular fate decisions.

      Associated diseases and disorders

      Mutations or loss of function in ARID1A has been strongly associated with various cancers including ovarian and endometrial cancers. In these contexts ARID1A dysfunction often occurs alongside other proteins such as p53 contributing to tumorigenesis by deregulating tumor suppressive pathways. It also holds a potential connection to neurodevelopmental disorders due to its role in chromatin remodeling and gene expression.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      6 product images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)

        This data was developed using Anti-ARID1A antibody [EPR13501-73] ab182561, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue (left image) labeling ARID1A using Anti-ARID1A antibody [EPR13501-73] ab182561 at 1/1000 dilution. Right image: human clear cell carcinoma of kidney with ARID1A mutation. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.

        Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)

        This data was developed using Anti-ARID1A antibody [EPR13501-73] ab182561, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of SH-SY5Y cells labeling ARID1A with Anti-ARID1A antibody [EPR13501-73] ab182561 at 1/500 and Goat anti rabbit IgG(Alexa Fluor®555) at 1/200. Image at the right stained with DAPI.

      • Flow Cytometry (Intracellular) - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)

        This data was developed using Anti-ARID1A antibody [EPR13501-73] ab182561, the same antibody clone in a different buffer formulation.

        Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling ARID1A with purified ab92512 at 1/500 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)

        This data was developed using Anti-ARID1A antibody [EPR13501-73] ab182561, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium without ARID1A mutation (left image) labeling ARID1A using Anti-ARID1A antibody [EPR13501-73] ab182561 at 1/1000 dilution. Right image: human adenocarcinoma of endometrium with ARID1A mutation. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.

        Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

      • ChIC/CUT&RUN sequencing - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617), expandable thumbnail

        ChIC/CUT&RUN sequencing - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)

        ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HCT116 cells and 5µg of Anti-ARID1A antibody [EPR13501-73] ab182561 [EPR13501-73]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

        Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.

        The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

        This data was developed using Anti-ARID1A antibody [EPR13501-73] ab182561, the same antibody clone in a different buffer formulation.

      • Western blot - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617), expandable thumbnail

        Western blot - Anti-ARID1A antibody [EPR13501-73] - BSA and Azide free (ab250617)

        This data was developed using Anti-ARID1A antibody [EPR13501-73] ab182561, the same antibody clone in a different buffer formulation.

        Blocking and diluting buffer: 5% NFDM/TBST

        ARID1A has many mutations which typically generate truncated proteins that are highly prone to degradation. (PMID: 21614196, PMID: 29486633, PMID: 34429326).

        All lanes: Western blot - Anti-ARID1A antibody [EPR13501-73] (Anti-ARID1A antibody [EPR13501-73] ab182561) at 1/1000 dilution

        Lane 1: ARID1 knockout HAP1 (Human chronic myelogenous leukemia near-haploid cell line) treated with 10uM MG-132 for 6 hours whole cell lysate at 20 µg

        Lane 2: Wild-type HAP1 (Human chronic myelogenous leukemia-haploid cell line) treated with 10uM MG-132 for 6 hours whole cell lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Predicted band size: 242 kDa

        Observed band size: 130-270 kDa

        Exposure time: 10s

        Blocking and diluting buffer: 5% NFDM/TBST

        ARID1A has many mutations which typically generate truncated proteins that are highly prone to degradation. (PMID: 21614196, PMID: 29486633, PMID: 34429326).

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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