Anti-ARID2 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-ARID2 antibody (AB245529)

ARID2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245529 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab245529 at 1 μg/ml.

Lane 1 : ab245529 (Batch 1) IP in HeLa whole cell lysate.

Lane 2 : ab245529 (Batch 2) IP in HeLa whole cell lysate.

Lane 3 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-ARID2 antibody (ab245529)

Predicted band size: 197 kDa

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• WB

Lab

Western blot - Anti-ARID2 antibody (AB245529)

False colour image of Western blot : Anti-ARID2 antibody staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab245529 was shown to bind specifically to ARID2. A band was observed at 220 kDa in wild-type HeLa cell lysates with no signal observed at this size in ARID2 knockout cell line ab265137 (knockout cell lysate ab257357). To generate this image, wild-type and ARID2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-ARID2 antibody (ab245529) at 1/2000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ARID2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ARID2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-arid2-knockout-hela-cell-line-ab265137'>ab265137</a>)

Lane 3:

HEK-293 cell lysate at 10 µg

Lane 4:

U-87 MG cell lysate at 10 µg

Predicted band size: 197 kDa

Observed band size: 220 kDa

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• WB

Supplier Data

Western blot - Anti-ARID2 antibody (AB245529)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-ARID2 antibody (ab245529) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 197 kDa

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Exposure time: 3min