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Rabbit Recombinant Monoclonal ARID2 antibody. Suitable for Flow Cyt (Intra), ICC, IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ARID2 aa 1750 to C-terminus.

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Images

Flow Cytometry (Intracellular) - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046), expandable thumbnail
  • Western blot - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046), expandable thumbnail
  • Immunocytochemistry - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human ARID2 aa 1750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q68CP9

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICCIHC-PWB
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes.

Alternative names

BAF200, KIAA1557, ARID2, AT-rich interactive domain-containing protein 2, ARID domain-containing protein 2, BRG1-associated factor 200, Zinc finger protein with activation potential, Zipzap/p200

Recommended products

Rabbit Recombinant Monoclonal ARID2 antibody. Suitable for Flow Cyt (Intra), ICC, IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ARID2 aa 1750 to C-terminus.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human ARID2 aa 1750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q68CP9
Clone number
BLR213K
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ARID2 also known as AT-rich interactive domain-containing protein 2 is a component of the SWI/SNF chromatin remodeling complex. It has a molecular mass of approximately 183 kDa. ARID2 is expressed in various tissues with high levels found in the liver kidney and testis. In the cell nucleus it functions as a DNA-binding protein playing a role in modulating chromatin structure through its interaction with other chromatin-associated proteins.

Biological function summary

ARID2 contributes to regulating gene expression by remodeling chromatin allowing transcription factors access to DNA. It acts as a part of the PBAF subtype of the SWI/SNF complex which includes proteins like BRG1 and BAF200. This complex influences transcriptional activation and repression aiding the regulation of genes important for cellular differentiation and development. Loss or mutation of ARID2 affects normal biological processes indicating its role in ensuring proper cellular function.

Pathways

ARID2 plays a role in the RAS signaling and the Hedgehog signaling pathways. It interacts with proteins such as BAF155 and BAF170 which are essential for recruiting and assembling the PBAF complex on chromatin. These pathways are critical for cellular proliferation and differentiation ensuring that cells respond accurately to signals which is vital for maintaining tissue homeostasis and proper organismal development.

Associated diseases and disorders

ARID2 mutations are linked to hepatocellular carcinoma and malignant melanoma. These mutations are associated with abnormalities in chromatin remodeling that lead to transcriptional dysregulation. The target is also connected to proteins like BRD9 and BRD7 within the context of cancer which are involved in tumor suppression. Alterations in ARID2 might contribute to the progression and aggressiveness of these cancers by disrupting normal chromatin dynamics and gene expression patterns.

Product promise

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4 product images

  • Flow Cytometry (Intracellular) - Anti-ARID2 antibody [BLR213K] - BSA free (ab314046), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ARID2 antibody [BLR213K] - BSA free (ab314046)

    This data was developed using the standard version of ab314046, the same antibody clone in a different buffer formulation.
    Detection of human ARID2 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells by Flow Cytometry (Intracellular).
    Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
    Antibody: Standard version of ab314046 (shaded red) or isotype control (unshaded).
    Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.

  • Western blot - Anti-ARID2 antibody [BLR213K] - BSA free (ab314046), expandable thumbnail

    Western blot - Anti-ARID2 antibody [BLR213K] - BSA free (ab314046)

    Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, MOLT-4, and SK-N-BE(2) cells prepared using NETN lysis buffer. Antibody:Rabbit anti-ARID2 recombinant monoclonal antibody [BLR213K] (A700-213 lot 1) used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A).

    All lanes: Western blot - Anti-ARID2 antibody [BLR213K] - BSA free (ab314046) at 1/1000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

    Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

    Lane 4: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

    Lane 5: SK-N-BE(2) (Human neuroblastoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Immunocytochemistry - Anti-ARID2 antibody [BLR213K] - BSA free (ab314046), expandable thumbnail

    Immunocytochemistry - Anti-ARID2 antibody [BLR213K] - BSA free (ab314046)

    Sample: FFPE section of LNCaP (Human prostate cancer cell line) cells.
    Antibody: Standard version of ab314046 at a 1/100 dilution.
    Secondary: HRP-conjugated goat anti-rabbit IgG.
    Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE cell sections.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID2 antibody [BLR213K] - BSA free (ab314046), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID2 antibody [BLR213K] - BSA free (ab314046)

    This data was developed using the standard version of ab314046, the same antibody clone in a different buffer formulation.
    Sample: FFPE section of human prostate carcinoma.
    Antibody: Standard version of ab314046 at a 1/100 dilution.
    Secondary: HRP-conjugated goat anti-rabbit IgG.
    Epitope retrieval with Tris-EDTA pH6.0 is recommended for FFPE tissue sections.

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