AB314046

Anti-ARID2 antibody [BLR213K] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal ARID2 antibody. Suitable for Flow Cyt (Intra), ICC, IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ARID2 aa 1750 to C-terminus.

View Alternative Names

BAF200, KIAA1557, ARID2, AT-rich interactive domain-containing protein 2, ARID domain-containing protein 2, BRG1-associated factor 200, Zinc finger protein with activation potential, Zipzap/p200

4 Images

Flow Cytometry (Intracellular) - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)

Detection of human ARID2 (shaded) in Jurkat cells by flow cytometry. Antibody : Rabbit anti-ARID2 recombinant monoclonal antibody [BLR0213K] (ab314046) or isotype control (unshaded). Secondary : DyLight^® 488-conjugated goat anti-rabbit IgG (ab96883).

Supplier Data

Immunocytochemistry - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)

Sample : FFPE section of LNCaP (Human prostate cancer cell line) cells. Antibody : Standard version of ab314046 at a 1/100 dilution. Secondary : HRP-conjugated goat anti-rabbit IgG. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE cell sections.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)

This data was developed using the standard version of ab314046, the same antibody clone in a different buffer formulation. Sample : FFPE section of human prostate carcinoma. Antibody : Standard version of ab314046 at a 1/100 dilution. Secondary : HRP-conjugated goat anti-rabbit IgG. Epitope retrieval with Tris-EDTA pH6.0 is recommended for FFPE tissue sections.

Supplier Data

Western blot - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)

Samples : Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, MOLT-4, and SK-N-BE(2) cells prepared using NETN lysis buffer. Antibody : Rabbit anti-ARID2 recombinant monoclonal antibody [BLR213K] (A700-213 lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 75 seconds. Lower Panel : Rabbit anti-COPB2 antibody (A304-523A).

All lanes:

Western blot - Anti-ARID2 antibody [BLR213K] - BSA free (ab314046) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

Lane 4:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

Lane 5:

SK-N-BE(2) (Human neuroblastoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR213K

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human ARID2 aa 1750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q68CP9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARID2 also known as AT-rich interactive domain-containing protein 2 is a component of the SWI/SNF chromatin remodeling complex. It has a molecular mass of approximately 183 kDa. ARID2 is expressed in various tissues with high levels found in the liver kidney and testis. In the cell nucleus it functions as a DNA-binding protein playing a role in modulating chromatin structure through its interaction with other chromatin-associated proteins.

Biological function summary

ARID2 contributes to regulating gene expression by remodeling chromatin allowing transcription factors access to DNA. It acts as a part of the PBAF subtype of the SWI/SNF complex which includes proteins like BRG1 and BAF200. This complex influences transcriptional activation and repression aiding the regulation of genes important for cellular differentiation and development. Loss or mutation of ARID2 affects normal biological processes indicating its role in ensuring proper cellular function.

Pathways

ARID2 plays a role in the RAS signaling and the Hedgehog signaling pathways. It interacts with proteins such as BAF155 and BAF170 which are essential for recruiting and assembling the PBAF complex on chromatin. These pathways are critical for cellular proliferation and differentiation ensuring that cells respond accurately to signals which is vital for maintaining tissue homeostasis and proper organismal development.

ARID2 mutations are linked to hepatocellular carcinoma and malignant melanoma. These mutations are associated with abnormalities in chromatin remodeling that lead to transcriptional dysregulation. The target is also connected to proteins like BRD9 and BRD7 within the context of cancer which are involved in tumor suppression. Alterations in ARID2 might contribute to the progression and aggressiveness of these cancers by disrupting normal chromatin dynamics and gene expression patterns.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes.

See full target information ARID2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARID2 antibody [BLR213K] - BSA free

Flow Cytometry (Intracellular) - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)

Immunocytochemistry - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)

Western blot - Anti-ARID2 antibody [BLR213K] - BSA free (AB314046)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise