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Rabbit Recombinant Monoclonal ARID3A antibody. Suitable for WB, IP, ChIP-seq and reacts with Recombinant fragment - Human, Human samples.

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Images

ChIP-sequencing - Anti-ARID3A antibody [EPR26964-29] (AB322894), expandable thumbnail
  • ChIP-sequencing - Anti-ARID3A antibody [EPR26964-29] (AB322894), expandable thumbnail
  • ChIP-sequencing - Anti-ARID3A antibody [EPR26964-29] (AB322894), expandable thumbnail
  • Immunoprecipitation - Anti-ARID3A antibody [EPR26964-29] (AB322894), expandable thumbnail
  • Western blot - Anti-ARID3A antibody [EPR26964-29] (AB322894), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPChIP-seq
Human
Tested
Tested
Tested
Recombinant fragment - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
8 µg for 107 Cells
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.

Alternative names

DRIL1, DRIL3, DRX, E2FBP1, ARID3A, AT-rich interactive domain-containing protein 3A, ARID domain-containing protein 3A, B-cell regulator of IgH transcription, Dead ringer-like protein 1, E2F-binding protein 1, Bright

Rabbit Recombinant Monoclonal ARID3A antibody. Suitable for WB, IP, ChIP-seq and reacts with Recombinant fragment - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26964-29
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • ChIP-sequencing - Anti-ARID3A antibody [EPR26964-29] (ab322894), expandable thumbnail

    ChIP-sequencing - Anti-ARID3A antibody [EPR26964-29] (ab322894)

    Chromatin was prepared from K-562 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab322894 [EPR26964-29]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

  • ChIP-sequencing - Anti-ARID3A antibody [EPR26964-29] (ab322894), expandable thumbnail

    ChIP-sequencing - Anti-ARID3A antibody [EPR26964-29] (ab322894)

    Chromatin was prepared from K-562 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab322894 [EPR26964-29]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

  • ChIP-sequencing - Anti-ARID3A antibody [EPR26964-29] (ab322894), expandable thumbnail

    ChIP-sequencing - Anti-ARID3A antibody [EPR26964-29] (ab322894)

    Chromatin was prepared from K-562 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab322894 [EPR26964-29]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

  • Immunoprecipitation - Anti-ARID3A antibody [EPR26964-29] (ab322894), expandable thumbnail

    Immunoprecipitation - Anti-ARID3A antibody [EPR26964-29] (ab322894)

    ARID3A was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab322894 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322894 at 1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

    Lane 2: ab322894 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab322894 in K-562 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    The band below 75 kDa is likely to represent truncated forms of ARID3A (PMID: 36008383). This has not been confirmed with additional tests.

    All lanes: Immunoprecipitation - Anti-ARID3A antibody [EPR26964-29] (ab322894) at 1/30 dilution

    All lanes: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 3s

  • Western blot - Anti-ARID3A antibody [EPR26964-29] (ab322894), expandable thumbnail

    Western blot - Anti-ARID3A antibody [EPR26964-29] (ab322894)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human ARID3B and ARID3C.

    All lanes: Western blot - Anti-ARID3A antibody [EPR26964-29] (ab322894) at 1/1000 dilution

    Lane 1: Human ARID3A fragment at 10 ng

    Lane 2: Human ARID3B recombinant protein at 10 ng

    Lane 3: Human ARID3C recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 75 kDa

    Exposure time: 180s

  • Western blot - Anti-ARID3A antibody [EPR26964-29] (ab322894), expandable thumbnail

    Western blot - Anti-ARID3A antibody [EPR26964-29] (ab322894)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The band below 75 kDa is likely to represent truncated forms of ARID3A (PMID: 36008383). This has not been confirmed with additional tests.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ARID3A antibody [EPR26964-29] (ab322894) at 1/1000 dilution

    Lane 1: Huh7 (human hepatocellular carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: Huh7 transfected with siRNA specifically targeting ARID3A whole cell lysate at 20 µg

    Lane 3: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 75 kDa, 36 kDa

    Exposure time: 15s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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