Anti-ARID3A antibody [EPR26964-29]

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-ARID3A antibody [EPR26964-29] (AB322894)

Chromatin was prepared from K-562 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab322894 [EPR26964-29]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-ARID3A antibody [EPR26964-29] (AB322894)

Chromatin was prepared from K-562 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab322894 [EPR26964-29]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-ARID3A antibody [EPR26964-29] (AB322894)

Chromatin was prepared from K-562 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab322894 [EPR26964-29]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• IP

Supplier Data

Immunoprecipitation - Anti-ARID3A antibody [EPR26964-29] (AB322894)

ARID3A was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab322894 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322894 at 1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : ab322894 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab322894 in K-562 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

The band below 75 kDa is likely to represent truncated forms of ARID3A (PMID : 36008383). This has not been confirmed with additional tests.

All lanes:

Immunoprecipitation - Anti-ARID3A antibody [EPR26964-29] (ab322894) at 1/30 dilution

All lanes:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-ARID3A antibody [EPR26964-29] (AB322894)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The band below 75 kDa is likely to represent truncated forms of ARID3A (PMID : 36008383). This has not been confirmed with additional tests.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ARID3A antibody [EPR26964-29] (ab322894) at 1/1000 dilution

Lane 1:

Huh7 (human hepatocellular carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

Huh7 transfected with siRNA specifically targeting ARID3A whole cell lysate at 20 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa,36 kDa

true

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-ARID3A antibody [EPR26964-29] (AB322894)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human ARID3B and ARID3C.

All lanes:

Western blot - Anti-ARID3A antibody [EPR26964-29] (ab322894) at 1/1000 dilution

Lane 1:

Human ARID3A fragment at 10 ng

Lane 2:

Human ARID3B recombinant protein at 10 ng

Lane 3:

Human ARID3C recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa

true

Exposure time: 180s