Anti-ARID3A antibody [EPR26964-8] - BSA and Azide free (Detector)
- Recombinant
- RabMAb
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Rabbit Recombinant Monoclonal ARID3A antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
View Alternative Names
DRIL1, DRIL3, DRX, E2FBP1, ARID3A, AT-rich interactive domain-containing protein 3A, ARID domain-containing protein 3A, B-cell regulator of IgH transcription, Dead ringer-like protein 1, E2F-binding protein 1, Bright
![Sandwich ELISA - Anti-ARID3A antibody [EPR26964-8] - BSA and Azide free (Detector) (AB320100)](https://content.abcam.com/products/images/arid3a-antibody-epr26964-8-bsa-and-azide-free-detector-ab320100--sandwich-elisa-img433972.jpg/_jcr_content/renditions/webp-768.webp)
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Reactivity data
Product details
ab320100 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ARID3A. The recommended pair for sandwich ELISA is:
Capture: ab320099, Human ARID3A Capture Antibody (unconjugated)
Detector: ab320100, Human ARID3A Detector Antibody (unconjugated)
The reference range value is 0.16-10 ng/mL.
The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ARID3A regulates distinct processes in cell differentiation and development. This protein is essential for normal B cell development and function facilitating the transition of B cells through various stages of maturation. ARID3A does not operate in isolation; it forms part of larger protein complexes that interact with other proteins to regulate transcription. By doing so it ensures specific gene expression patterns necessary for correct cell lineage determination.
Pathways
Multiple cellular systems necessitate ARID3A's involvement. It acts within the B cell receptor (BCR) signaling pathway where it collaborates with other proteins such as BLNK to promote B cell responses. ARID3A also participates in the cell cycle regulation pathway influencing cell cycle progression by directly affecting the transcription of genes that regulate cell division.
Product protocols
- Visit the General protocols
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Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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