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AB320099

Anti-ARID3A + ARID3C antibody [EPR26964-69] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal ARID3A antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

DRIL1, DRIL3, DRX, E2FBP1, ARID3A, AT-rich interactive domain-containing protein 3A, ARID domain-containing protein 3A, B-cell regulator of IgH transcription, Dead ringer-like protein 1, E2F-binding protein 1, Bright, AT-rich interactive domain-containing protein 3C, ARID domain-containing protein 3C

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Sandwich ELISA - Anti-ARID3A + ARID3C antibody [EPR26964-69] - BSA and Azide free (Capture) (AB320099)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ARID3A + ARID3C antibody [EPR26964-69] - BSA and Azide free (Capture) (AB320099)

Sandwich ELISA of ab320098 with the capture antibody ab320099 dilution at 2 ug/mL and detector antibody ab320100 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26964-69

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has 100% cross reaction with human ARID3C, but does not cross-react with human ARID3B.

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Reactivity data

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Product details

ab320099 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ARID3A. The recommended pair for sandwich ELISA is:

Capture: ab320099, Human ARID3A Capture Antibody (unconjugated)

Detector: ab320100, Human ARID3A Detector Antibody (unconjugated)

The reference range value is 0.16-10 ng/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARID3A also known as Bright or DRIL1 and ARID3C are members of the ARID (AT-rich interaction domain) family of DNA-binding proteins. These proteins function as transcription factors and show a preference for binding to AT-rich DNA sequences. ARID3A has a molecular mass of approximately 67 kDa. Both proteins are expressed in various tissues but ARID3A shows higher expression in B-cells and early hematopoietic cells which indicates a role in immune function as well as development.
Biological function summary

ARID3A and ARID3C participate in regulating transcription through chromatin remodeling and gene expression control. ARID3A forms part of a larger DNA-binding complex which includes other transcriptional regulators that modulate gene expression linked to B-cell differentiation and immune response. In contrast less is known about ARID3C yet it is thought to share some functional overlap with ARID3A due to similar sequences and domain structure. Despite this ARID3C's expression pattern and biological role remain less well-defined compared to its counterpart ARID3A.

Pathways

ARID3A and ARID3C are involved in pathways related to immune response and cell cycle regulation. ARID3A plays an important role in B-cell development influencing pathways like the B-cell receptor signaling pathway which is critical for proper immune function. It also interacts with proteins such as BLNK and BTK which are important in transmitting signals essential for B-cell maturation. The precise pathway associations of ARID3C are under investigation but its similarity to ARID3A suggests overlapping pathways are likely.

ARID3A and ARID3C have implications in autoimmune diseases and cancer development. ARID3A is connected with systemic lupus erythematosus (SLE) a complex autoimmune disorder where its abnormal expression disrupts immune tolerance. Furthermore both ARID3A and ARID3C have been associated with specific cancers particularly due to their roles in regulating genes involved in cell proliferation. Aberrant expression of ARID3A contributes to leukemogenesis where it interacts with oncogenes such as MYC. Understanding these connections can help in developing targeted therapies for related conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.
See full target information ARID3A

Additional targets

ARID3C
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Product promise

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For full details, please see our Terms & Conditions

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