Rabbit Polyclonal ARID5A antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AT-rich interactive domain-containing protein 5A aa 1-300.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
WB | IHC-P | |
---|---|---|
Human | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
DNA-binding protein that may regulate transcription and act as a repressor by binding to AT-rich stretches in the promoter region of target genes (PubMed:8649988). May positively regulate chondrocyte-specific transcription such as of COL2A1 in collaboration with SOX9 and positively regulate histone H3 acetylation at chondrocyte-specific genes. May stimulate early-stage chondrocyte differentiation and inhibit later stage differention (By similarity). Can repress ESR1-mediated transcriptional activation; proposed to act as corepressor for selective nuclear hormone receptors (PubMed:15941852). As an RNA-binding protein, involved in the regulation of inflammatory response by stabilizing selective inflammation-related mRNAs, such as STAT3 and TBX21 (By similarity). Also stabilizes IL6 mRNA (PubMed:32209697). Binds to stem loop structures located in the 3'UTRs of IL6, STAT3 and TBX21 mRNAs; at least for STAT3 prevents binding of ZC3H12A to the mRNA stem loop structure thus inhibiting its degradation activity. Contributes to elevated IL6 levels possibly implicated in autoimmunity processes. IL6-dependent stabilization of STAT3 mRNA may promote differentiation of naive CD4+ T-cells into T-helper Th17 cells. In CD4+ T-cells may also inhibit RORC-induced Th17 cell differentiation independently of IL6 signaling. Stabilization of TBX21 mRNA contributes to elevated interferon-gamma secretion in Th1 cells possibly implicated in the establishment of septic shock (By similarity). Stabilizes TNFRSF4/OX40 mRNA by binding to the conserved stem loop structure in its 3'UTR; thereby competing with the mRNA-destabilizing functions of RC3H1 and endoribonuclease ZC3H12A (By similarity).
MRF1, ARID5A, MRF1, AT-rich interactive domain-containing protein 5A, ARID domain-containing protein 5A, Modulator recognition factor 1, MRF-1
Rabbit Polyclonal ARID5A antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AT-rich interactive domain-containing protein 5A aa 1-300.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
ARID5A also known as AT-rich interactive domain-containing protein 5A is a transcriptional regulator with a molecular weight of about 61 kDa. This protein functions by binding to DNA and regulating gene expression. ARID5A is expressed in various tissues including the liver and immune cells where it influences different cellular processes. It plays a significant role in modulating pathways that involve inflammation and cellular responses.
ARID5A acts as a critical component in the immune response involved primarily in the regulation of mRNA stability especially in pro-inflammatory cytokines like IL-6 and IL-13. ARID5A often functions within larger protein complexes that fine-tune the immune response by affecting transcriptional and post-transcriptional processes. Through these actions ARID5A impacts how cells react to inflammatory signals and stress stimuli.
ARID5A significantly contributes to the JAK-STAT and NF-kB signaling pathways. In the JAK-STAT pathway ARID5A influences the stability and expression of inflammatory cytokines working closely with proteins like STAT3. Its interaction in the NF-kB pathway involves controlling the transcription of genes central to inflammation and immunity. Both pathways are essential for maintaining immune homeostasis and proper inflammatory responses.
ARID5A has connections to autoimmune conditions and inflammation-related diseases. Altered ARID5A expression or function shows an association with rheumatoid arthritis due to its role in regulating pro-inflammatory cytokines. Additionally ARID5A relates to inflammatory bowel disease where it impacts the immune response within the gastrointestinal tract. Proteins such as IL-6 and IL-13 regulated by ARID5A also become key players in these disorders highlighting the protein's critical regulatory function in disease contexts.
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7.5% SDS PAGE
All lanes: Western blot - Anti-ARID5A antibody (ab151449) at 1/5000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Lane 3: HeLa whole cell lysate at 30 µg
Predicted band size: 64 kDa
Immunohistochemical analysis of paraffin-embedded 59T xenograft tissue labeling ARID5A with ab151449 at 1/500 dilution.
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