Rabbit Polyclonal ARID5B antibody. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARID5B.
View Alternative Names
DESRT, MRF2, ARID5B, AT-rich interactive domain-containing protein 5B, ARID domain-containing protein 5B, MRF1-like protein, Modulator recognition factor 2, MRF-2
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-ARID5B antibody (AB226776)
Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling ARID5B protein at nucleus using ab226776 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID5B antibody (AB226776)
Immunohistochemical analysis of mouse intestine tissue stained for ARID5B protein at nucleus with ab226776 at a 1/500 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID5B antibody (AB226776)
Immunohistochemical analysis of mouse intestine tissue stained for ARID5B protein at nucleus with ab226776 at a 1/500 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min.
- WB
Supplier Data
Western blot - Anti-ARID5B antibody (AB226776)
5% SDS-PAGE gel.
All lanes:
Western blot - Anti-ARID5B antibody (ab226776) at 1/500 dilution
Lane 1:
HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 2:
HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract at 30 µg
Predicted band size: 132 kDa
false
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Reactivity data
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The activities of AT-rich sequences within the gene regulatory network depend significantly on transcription factors. ARID5B plays an important role in modulating the expression of genes involved in cell differentiation and development. It functions within a complex of proteins that participate in chromatin remodeling which alters the accessibility of chromatin to the transcriptional machinery. Through these interactions ARID5B ensures precise control over gene expression necessary for proper cellular function and organism development.
Pathways
Transcription factors like ARID5B integrate signals from various regulatory networks. ARID5B engages in the Wnt signaling and Notch pathways influencing stem cell differentiation and maintaining tissue homeostasis. In these pathways ARID5B interacts with other proteins like TCF/LEF transcription factors in the Wnt pathway and NICD (Notch intracellular domain) in the Notch pathway coordinating the transcriptional response required for cell fate determination.
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Target data
Publications (1)
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Oncogenesis 7:84 PubMed30420689
2018
Applications
Unspecified application
Species
Unspecified reactive species
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