Rabbit Polyclonal ARID5B antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARID5B.
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Publications
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- Oncogenesis 7:842018Aberrant ARID5B expression and its association with Ikaros dysfunction in acute lymphoblastic leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Ge et. al.PubMed 30420689
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARID5B. The exact immunogen used to generate this antibody is proprietary information. Database link Q14865
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Target data
Function
Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer.
Alternative names
AT-rich interactive domain-containing protein 5B, ARID domain-containing protein 5B, MRF1-like protein, Modulator recognition factor 2, MRF-2, MRF2, DESRT, ARID5B
Recommended products
Rabbit Polyclonal ARID5B antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARID5B.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARID5B. The exact immunogen used to generate this antibody is proprietary information. Database link Q14865
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARID5B also known as AT-rich interactive domain-containing protein 5B functions as a transcription factor. This protein with a mass of approximately 132 kilodaltons interacts with DNA to regulate gene expression by binding to specific AT-rich sequences. ARID5B localizes in the nucleus and exhibits expression in various tissues including the lungs spleen and liver highlighting its involvement in multiple biological processes.
- Biological function summary
The activities of AT-rich sequences within the gene regulatory network depend significantly on transcription factors. ARID5B plays an important role in modulating the expression of genes involved in cell differentiation and development. It functions within a complex of proteins that participate in chromatin remodeling which alters the accessibility of chromatin to the transcriptional machinery. Through these interactions ARID5B ensures precise control over gene expression necessary for proper cellular function and organism development.
- Pathways
Transcription factors like ARID5B integrate signals from various regulatory networks. ARID5B engages in the Wnt signaling and Notch pathways influencing stem cell differentiation and maintaining tissue homeostasis. In these pathways ARID5B interacts with other proteins like TCF/LEF transcription factors in the Wnt pathway and NICD (Notch intracellular domain) in the Notch pathway coordinating the transcriptional response required for cell fate determination.
- Associated diseases and disorders
ARID5B mutations or dysregulation have associations with certain types of cancer and autoimmune diseases. Acute lymphoblastic leukemia (ALL) shows links to altered ARID5B function contributing to abnormal cell proliferation. Additionally variations in ARID5B influence the risk of developing type 1 diabetes where it interacts with other immune-related proteins such as HLA-DRB1. Researchers study these interactions to understand disease mechanisms and explore potential therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-ARID5B antibody (ab226776)
5% SDS-PAGE gel.
All lanes: Western blot - Anti-ARID5B antibody (ab226776) at 1/500 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract at 30 µg
Predicted band size: 132 kDa
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