JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB3891

Anti-ARIH1 antibody

4

(1 Review)

|

(5 Publications)

Goat Polyclonal ARIH1 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human ARIH1 aa 500 to C-terminus.

View Alternative Names

ARI, MOP6, UBCH7BP, HUSSY-27, ARIH1, E3 ubiquitin-protein ligase ARIH1, H7-AP2, HHARI, Monocyte protein 6, Protein ariadne-1 homolog, UbcH7-binding protein, UbcM4-interacting protein, Ubiquitin-conjugating enzyme E2-binding protein 1, MOP-6, ARI-1

2 Images
Western blot - Anti-ARIH1 antibody (AB3891)
  • WB

AbReview38222****

Western blot - Anti-ARIH1 antibody (AB3891)

Lanes 1 and 2 - 12% SDS-PAGE.

Lane 3 - 4-15% SDS-PAGE.

Lanes 1 and 3 - developed with colorimetric substrate.

Lane 2 - developed with ECF+.

All lanes:

Western blot - Anti-ARIH1 antibody (ab3891) at 1/1500 dilution

Lanes 1 - 2:

NIH3T3 whole cell lysate at 30 µg

Lane 3:

Partially purified NIH3T3 protein mixture at 10 µg

Secondary

All lanes:

Alkaline Phosphatase-conjugated anti-goat IgG polyclonal at 1/15000 dilution

Predicted band size: 64 kDa

Observed band size: 45 kDa,50 kDa,65 kDa

false

This image is courtesy of an Abreview submitted by T Lawson

Western blot - Anti-ARIH1 antibody (AB3891)
  • WB

Unknown

Western blot - Anti-ARIH1 antibody (AB3891)

ab3891 staining (0.5μg/ml) of U937 lysate (RIPA buffer, 30μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab3891 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

All lanes:

Western blot - Anti-ARIH1 antibody (ab3891)

Predicted band size: 64 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ARIH1 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4X5

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }
style
left-column
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARIH1 also known as Human Ubiquitin-Protein Ligase ARIH1 is a 55-kDa protein involved in the ubiquitination process. Ubiquitination is a process that tags proteins for degradation. ARIH1 functions as an E3 ubiquitin-protein ligase facilitating the transfer of ubiquitin to specific substrate proteins. It acts with ubiquitin-conjugating enzymes mediating the attachment of ubiquitin molecules to protein targets. ARIH1 is mainly expressed in the cytoplasm and nucleus of cells with higher expression detected in the brain and testes.
Biological function summary

ARIH1 plays a role in the regulation of several cellular processes such as protein degradation signal transduction and DNA repair. It participates in the formation of the ARIH1-E2 ubiquitin-protein ligase complex which modulates protein stability by marking them for proteasomal degradation. This complex ensures the controlled turnover of key regulatory proteins to maintain cellular homeostasis. ARIH1 also influences the function of other important molecules through its ubiquitination activity impacting cellular responses to stress.

Pathways

ARIH1 gets involved in the ubiquitin-proteasome system which orchestrates the degradation of unneeded or damaged proteins. ARIH1 operates within this pathway interacting with proteins like the E2 conjugating enzyme UBE2L3. Moreover it participates in the NEDDylation pathway which adds another layer of regulation to protein stability and function. Within these pathways ARIH1 ensures effective control over multiple cellular mechanisms thereby supporting cellular function.

ARIH1 associates with conditions like cancer and neurodegenerative diseases. In cancer altered ubiquitination processes involving ARIH1 may disrupt normal cellular regulation contributing to tumorigenesis. Additionally ARIH1's dysregulation impacts neurodegenerative diseases potentially linked to improper protein degradation in neurons. ARIH1's interactions with proteins such as p53 and PARK2 further connect it to these disorders highlighting its role in the pathological processes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed : 15236971, PubMed : 21532592, PubMed : 23707686, PubMed : 24076655, PubMed : 27565346). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates : associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets (PubMed : 27565346). The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2 (PubMed : 27565346). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes (PubMed : 24076655, PubMed : 27565346). Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear (PubMed : 25624349). According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest (PubMed : 25624349). According to another report EIF4E2 ubiquitination leads to its subsequent degradation (PubMed : 14623119). Acts as the ligase involved in ISGylation of EIF4E2 (PubMed : 17289916). In vitro, controls the degradation of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex member SUN2 and may therefore have a role in the formation and localization of the LINC complex, and as a consequence, nuclear subcellular localization and nuclear morphology (PubMed : 29689197).
See full target information ARIH1
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 45:226-244.e8 PubMed29689197

2018

Ari-1 Regulates Myonuclear Organization Together with Parkin and Is Associated with Aortic Aneurysms.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Li Tan,Nele A Haelterman,Callie S Kwartler,Ellen S Regalado,Pei-Tseng Lee,Sonal Nagarkar-Jaiswal,Dong-Chuan Guo,Lita Duraine,Michael F Wangler,Michael J Bamshad,Deborah A Nickerson,Guang Lin,Dianna M Milewicz,Hugo J Bellen

EMBO reports 18:1572-1585 PubMed28784601

2017

Expanding the host cell ubiquitylation machinery targeting cytosolic .

Applications

Unspecified application

Species

Unspecified reactive species

Mira Polajnar,Marina S Dietz,Mike Heilemann,Christian Behrends

Cell 166:1198-1214.e24 PubMed27565346

2016

Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel C Scott,David Y Rhee,David M Duda,Ian R Kelsall,Jennifer L Olszewski,Joao A Paulo,Annemieke de Jong,Huib Ovaa,Arno F Alpi,J Wade Harper,Brenda A Schulman

Journal of neurochemistry 103:2354-68 PubMed17883392

2007

The effects of oxidative stress on parkin and other E3 ligases.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J LaVoie,Giuseppe P Cortese,Beth L Ostaszewski,Michael G Schlossmacher

The Journal of biological chemistry 282:12310-8 PubMed17329252

2007

Relative sensitivity of parkin and other cysteine-containing enzymes to stress-induced solubility alterations.

Applications

Unspecified application

Species

Unspecified reactive species

Esther S P Wong,Jeanne M M Tan,Cheng Wang,Zhenshui Zhang,Shiam-Peng Tay,Norazean Zaiden,Han Seok Ko,Valina L Dawson,Ted M Dawson,Kah-Leong Lim
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com