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AB248635

Anti-ARIH2/TRIAD1 antibody [EPR7670] - BSA and Azide free

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Rabbit Recombinant Monoclonal ARIH2/TRIAD1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

ARI2, TRIAD1, HT005, ARIH2, E3 ubiquitin-protein ligase ARIH2, ARI-2, Protein ariadne-2 homolog, Triad1 protein

3 Images
Flow Cytometry (Intracellular) - Anti-ARIH2/TRIAD1 antibody [EPR7670] - BSA and Azide free (AB248635)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ARIH2/TRIAD1 antibody [EPR7670] - BSA and Azide free (AB248635)

This data was developed using ab133744, the same antibody clone in a different buffer formulation.

Intracellular flow cytometrical analysis of permeabilized K562 cells labelling ARIH2/TRIAD1 with ab133744 at 1/10 (red) with rabbit IgG negative control (green).

Western blot - Anti-ARIH2/TRIAD1 antibody [EPR7670] - BSA and Azide free (AB248635)
  • WB

Unknown

Western blot - Anti-ARIH2/TRIAD1 antibody [EPR7670] - BSA and Azide free (AB248635)

This data was developed using ab133744, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARIH2/TRIAD1 antibody [EPR7670] (<a href='/en-us/products/primary-antibodies/arih2-triad1-antibody-epr7670-ab133744'>ab133744</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 57 kDa

Observed band size: 58 kDa

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OI-RD Scanning - Anti-ARIH2/TRIAD1 antibody [EPR7670] - BSA and Azide free (AB248635)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ARIH2/TRIAD1 antibody [EPR7670] - BSA and Azide free (AB248635)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7670

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248635 is the carrier-free version of ab133744.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARIH2 also known as TRIAD1 is a RING-between-RING (RBR) E3 ubiquitin-protein ligase. It weights approximately 65 kDa. This protein facilitates the transfer of ubiquitin from E2 enzymes to substrates marking them for proteasomal degradation. ARIH2 is located mostly in the cytoplasm and nucleoplasm with high expression in immune cells such as macrophages and dendritic cells. It regulates ubiquitination which is important for controlling protein levels in the cell affecting cellular signal pathways and homeostasis.
Biological function summary

ARIH2 acts as an essential modulator of immune responses. By associating with the LUBAC complex (linear ubiquitin chain assembly complex) this protein contributes to the linear ubiquitination of specific substrates modulating immune signaling. Its activity affects the NF-κB signaling pathway a vital pathway in inflammation and immunity. ARIH2 influences anti-inflammatory responses and may balance immune homeostasis making it a critical player in immune system regulation.

Pathways

ARIH2 plays a significant role in the ubiquitylation pathway and the NF-κB signaling pathway. The protein cooperates with other members of the pathway like HOIP and SHARPIN to mediate immune responses through linear ubiquitin chains. This regulation influences the activation of NF-κB leading to changes in gene expression related to immune and inflammatory responses. ARIH2 is also involved in the regulation of the Wnt signaling pathway where it interacts with various signaling proteins to modulate developmental processes and cellular growth.

ARIH2 has implications in autoimmune diseases and cancer. Its regulation of immune responses connects it to autoimmune conditions where the body's immune system attacks its own cells. By influencing NF-κB signaling through proteins like LUBAC ARIH2 affects inflammation contributing to the pathogenesis of autoimmune diseases. In cancer ARIH2's role in protein degradation pathways can alter cell growth and survival connecting it to tumor development and progression. Its interaction with proteins such as HOIP in these pathways suggests its potential as a therapeutic target for both autoimmune disorders and cancer.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed : 16118314, PubMed : 17646546, PubMed : 19340006, PubMed : 24076655, PubMed : 33268465, PubMed : 34518685, PubMed : 38418882). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates : associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (PubMed : 33268465, PubMed : 34518685, PubMed : 38418882). The initial ubiquitin is then elongated (PubMed : 33268465). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the cullin-5 (CUL5) component of the ECS complex (PubMed : 24076655). Together with the ECS(ASB9) complex, catalyzes ubiquitination of CKB (PubMed : 33268465). Promotes ubiquitination of DCUN1D1 (PubMed : 30587576). Mediates 'Lys-6', 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed : 16118314, PubMed : 17646546, PubMed : 19340006). May play a role in myelopoiesis (PubMed : 19340006).. (Microbial infection) Following infection by HIV-1 virus, acts together with a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) hijacked by the HIV-1 Vif protein, to catalyze ubiquitination and degradation of APOBEC3F and APOBEC3G.
See full target information ARIH2
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Product promise

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