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Rabbit Recombinant Monoclonal ARIH2/TRIAD1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Predicted
Not recommended
Predicted
Rat
Not recommended
Not recommended
Predicted
Not recommended
Predicted

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:16118314, PubMed:17646546, PubMed:19340006, PubMed:24076655). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (By similarity). The initial ubiquitin is then elongated (By similarity). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the ECS complex (PubMed:24076655). Mediates 'Lys-6', 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed:16118314, PubMed:17646546, PubMed:19340006). May play a role in myelopoiesis (PubMed:19340006).

Alternative names

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Rabbit Recombinant Monoclonal ARIH2/TRIAD1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR7670

Purification technique

Affinity purification

Dissociation constant

7.8 x 10-10 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248635 is the carrier-free version of ab133744.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Biological function summary

ARIH2 acts as an essential modulator of immune responses. By associating with the LUBAC complex (linear ubiquitin chain assembly complex) this protein contributes to the linear ubiquitination of specific substrates modulating immune signaling. Its activity affects the NF-κB signaling pathway a vital pathway in inflammation and immunity. ARIH2 influences anti-inflammatory responses and may balance immune homeostasis making it a critical player in immune system regulation.

Activity summary

ARIH2 also known as TRIAD1 is a RING-between-RING (RBR) E3 ubiquitin-protein ligase. It weights approximately 65 kDa. This protein facilitates the transfer of ubiquitin from E2 enzymes to substrates marking them for proteasomal degradation. ARIH2 is located mostly in the cytoplasm and nucleoplasm with high expression in immune cells such as macrophages and dendritic cells. It regulates ubiquitination which is important for controlling protein levels in the cell affecting cellular signal pathways and homeostasis.

Pathways

ARIH2 plays a significant role in the ubiquitylation pathway and the NF-κB signaling pathway. The protein cooperates with other members of the pathway like HOIP and SHARPIN to mediate immune responses through linear ubiquitin chains. This regulation influences the activation of NF-κB leading to changes in gene expression related to immune and inflammatory responses. ARIH2 is also involved in the regulation of the Wnt signaling pathway where it interacts with various signaling proteins to modulate developmental processes and cellular growth.

Associated diseases and disorders

ARIH2 has implications in autoimmune diseases and cancer. Its regulation of immune responses connects it to autoimmune conditions where the body's immune system attacks its own cells. By influencing NF-κB signaling through proteins like LUBAC ARIH2 affects inflammation contributing to the pathogenesis of autoimmune diseases. In cancer ARIH2's role in protein degradation pathways can alter cell growth and survival connecting it to tumor development and progression. Its interaction with proteins such as HOIP in these pathways suggests its potential as a therapeutic target for both autoimmune disorders and cancer.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-ARIH2/TRIAD1 antibody [EPR7670] - BSA and Azide free (ab248635), expandable thumbnail

    Western blot - Anti-ARIH2/TRIAD1 antibody [EPR7670] - BSA and Azide free (ab248635)

    This data was developed using ab133744, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-ARIH2/TRIAD1 antibody [EPR7670] (AB133744) at 1/1000 dilution

    Lane 1: Jurkat cell lysate at 10 µg

    Lane 2: K562 cell lysate at 10 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 57 kDa

    Observed band size: 58 kDa

  • Flow Cytometry (Intracellular) - Anti-ARIH2/TRIAD1 antibody [EPR7670] - BSA and Azide free (ab248635), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ARIH2/TRIAD1 antibody [EPR7670] - BSA and Azide free (ab248635)

    This data was developed using ab133744, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometrical analysis of permeabilized K562 cells labelling ARIH2/TRIAD1 with ab133744 at 1/10 (red) with rabbit IgG negative control (green).

  • OI-RD Scanning - Anti-ARIH2/TRIAD1 antibody [EPR7670] - BSA and Azide free (ab248635), expandable thumbnail

    OI-RD Scanning - Anti-ARIH2/TRIAD1 antibody [EPR7670] - BSA and Azide free (ab248635)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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