AB133653

Anti-ARIH2/TRIAD1 antibody [EPR7671]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal ARIH2/TRIAD1 antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 2 publications.

View Alternative Names

ARI2, TRIAD1, HT005, ARIH2, E3 ubiquitin-protein ligase ARIH2, ARI-2, Protein ariadne-2 homolog, Triad1 protein

2 Images

Unknown

Western blot - Anti-ARIH2/TRIAD1 antibody [EPR7671] (AB133653)

All lanes:

Western blot - Anti-ARIH2/TRIAD1 antibody [EPR7671] (ab133653) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

PC12 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 57 kDa

false

OI-RD Scanning - Anti-ARIH2/TRIAD1 antibody [EPR7671] (AB133653)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ARIH2/TRIAD1 antibody [EPR7671] (AB133653)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7671

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARIH2 also known as TRIAD1 is a RING-between-RING (RBR) E3 ubiquitin-protein ligase. It weights approximately 65 kDa. This protein facilitates the transfer of ubiquitin from E2 enzymes to substrates marking them for proteasomal degradation. ARIH2 is located mostly in the cytoplasm and nucleoplasm with high expression in immune cells such as macrophages and dendritic cells. It regulates ubiquitination which is important for controlling protein levels in the cell affecting cellular signal pathways and homeostasis.

Biological function summary

ARIH2 acts as an essential modulator of immune responses. By associating with the LUBAC complex (linear ubiquitin chain assembly complex) this protein contributes to the linear ubiquitination of specific substrates modulating immune signaling. Its activity affects the NF-κB signaling pathway a vital pathway in inflammation and immunity. ARIH2 influences anti-inflammatory responses and may balance immune homeostasis making it a critical player in immune system regulation.

Pathways

ARIH2 plays a significant role in the ubiquitylation pathway and the NF-κB signaling pathway. The protein cooperates with other members of the pathway like HOIP and SHARPIN to mediate immune responses through linear ubiquitin chains. This regulation influences the activation of NF-κB leading to changes in gene expression related to immune and inflammatory responses. ARIH2 is also involved in the regulation of the Wnt signaling pathway where it interacts with various signaling proteins to modulate developmental processes and cellular growth.

ARIH2 has implications in autoimmune diseases and cancer. Its regulation of immune responses connects it to autoimmune conditions where the body's immune system attacks its own cells. By influencing NF-κB signaling through proteins like LUBAC ARIH2 affects inflammation contributing to the pathogenesis of autoimmune diseases. In cancer ARIH2's role in protein degradation pathways can alter cell growth and survival connecting it to tumor development and progression. Its interaction with proteins such as HOIP in these pathways suggests its potential as a therapeutic target for both autoimmune disorders and cancer.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed : 16118314, PubMed : 17646546, PubMed : 19340006, PubMed : 24076655, PubMed : 33268465, PubMed : 34518685, PubMed : 38418882). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates : associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (PubMed : 33268465, PubMed : 34518685, PubMed : 38418882). The initial ubiquitin is then elongated (PubMed : 33268465). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the cullin-5 (CUL5) component of the ECS complex (PubMed : 24076655). Together with the ECS(ASB9) complex, catalyzes ubiquitination of CKB (PubMed : 33268465). Promotes ubiquitination of DCUN1D1 (PubMed : 30587576). Mediates 'Lys-6', 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed : 16118314, PubMed : 17646546, PubMed : 19340006). May play a role in myelopoiesis (PubMed : 19340006).. (Microbial infection) Following infection by HIV-1 virus, acts together with a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) hijacked by the HIV-1 Vif protein, to catalyze ubiquitination and degradation of APOBEC3F and APOBEC3G.

See full target information ARIH2

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 298:102443 PubMed36055408

2022

Ubiquitin ligase Triad1 promotes neurite outgrowth by inhibiting MDM2-mediated ubiquitination of the neuroprotective factor pleiotrophin.

Applications

Unspecified application

Species

Unspecified reactive species

Chunshuai Wu,Guanhua Xu,Guofeng Bao,Hong Gao,Jiajia Chen,Jinlong Zhang,Chu Chen,Hongxiang Hong,Pengfei Xue,Jiawei Jiang,Yang Liu,Jiayi Huang,Yue Sun,Jiawei Fu,Yiqiu Li,Zhiming Cui

Journal of neural transmission (Vienna, Austria : 1996) 128:701-709 PubMed33890175

2021

Cortical copper transporter expression in schizophrenia: interactions of risk gene dysbindin-1.

Applications

Unspecified application

Species

Unspecified reactive species

Kirsten E Schoonover,William M Kennedy,Rosalinda C Roberts

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARIH2/TRIAD1 antibody [EPR7671]

Western blot - Anti-ARIH2/TRIAD1 antibody [EPR7671] (AB133653)

OI-RD Scanning - Anti-ARIH2/TRIAD1 antibody [EPR7671] (AB133653)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise