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AB155982

Anti-ARL 1 antibody [EPR10595]

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(2 Publications)

Rabbit Recombinant Monoclonal ARL 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

ADP-ribosylation factor-like protein 1, ARL1

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (AB155982)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (AB155982)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling ARL 1 with ab155982 at 1/50 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (AB155982)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (AB155982)

ab155982 showing +ve staining in Human endometrial carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ARL 1 antibody [EPR10595] (AB155982)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ARL 1 antibody [EPR10595] (AB155982)

ICC/IF image of ab155982 stained CaCo2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab155982 at 10μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (AB155982)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (AB155982)

ab155982 showing +ve staining in Human normal prostate.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (AB155982)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (AB155982)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ARL 1 with ab155982 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (AB155982)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (AB155982)

ab155982 showing +ve staining in Human gastric adenocarcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (AB155982)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] (AB155982)

ab155982 showing +ve staining in Human normal brain.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-ARL 1 antibody [EPR10595] (AB155982)
  • WB

Unknown

Western blot - Anti-ARL 1 antibody [EPR10595] (AB155982)

All lanes:

Western blot - Anti-ARL 1 antibody [EPR10595] (ab155982) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 20 kDa

false

  • Carrier free

    Anti-ARL 1 antibody [EPR10595] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10595

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARL 1 also known as ADP-ribosylation factor-like protein 1 is a small GTPase of the Arf family. It has a molecular mass of about 20 kDa. This protein is ubiquitously expressed in human tissues with significant expression observed in the Golgi apparatus. ARL 1 regulates intracellular vesicular trafficking and plays a role in the structural integrity of the Golgi complex.
Biological function summary

ARL 1 interacts with multiple effectors to perform its roles in the cell. It becomes part of a complex with Golgi-associated proteins and participates in the regulation of membrane traffic between the Golgi and endosome. This protein associates with other small GTPases to control the localization and function of Golgi resident proteins impacting processes like glycosylation and secretion.

Pathways

ARL 1 is involved in important intracellular traffic pathways such as the Golgi to plasma membrane transport. It works within a network with proteins such as ARL2 and ARF1 influencing the COPI-coated vesicle cycle. ARL 1 interacts with pathways responsible for maintaining the Golgi structure and managing the trafficking of proteins and lipids.

ARL 1 has connections to diseases affecting the Golgi apparatus and vesicular transport like neurodegenerative disorders and cancer. It is associated with proteins like Golgin-97 and Rab6 in these contexts. Deregulation of ARL 1 function can result in altered cellular trafficking contributing to the pathogenesis of these conditions by impacting protein and lipid mislocalization.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTP-binding protein that recruits several effectors, such as golgins, arfaptins and Arf-GEFs to the trans-Golgi network, and modulates their functions at the Golgi complex (PubMed : 21239483, PubMed : 22679020, PubMed : 27373159, PubMed : 27436755, PubMed : 9624189). Plays thereby a role in a wide range of fundamental cellular processes, including cell polarity, innate immunity, or protein secretion mediated by arfaptins, which were shown to play a role in maintaining insulin secretion from pancreatic beta cells (PubMed : 22981988).
See full target information ARL1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2036 PubMed40021625

2025

Bone morphogenetic protein (BMP) signaling determines neuroblastoma cell fate and sensitivity to retinoic acid.

Applications

Unspecified application

Species

Unspecified reactive species

Min Pan,Yinwen Zhang,William C Wright,Xueying Liu,Barbara Passaia,Duane Currier,Jonathan Low,Richard H Chapple,Jacob A Steele,Jon P Connelly,Bensheng Ju,Emily Plyler,Meifen Lu,Allister J Loughran,Lei Yang,Brian J Abraham,Shondra M Pruett-Miller,Burgess Freeman,George E Campbell,Michael A Dyer,Taosheng Chen,Elizabeth Stewart,Selene Koo,Heather Sheppard,John Easton,Paul Geeleher

Methods in molecular biology (Clifton, N.J.) 2557:453-493 PubMed36512231

2022

Common Markers and Small Molecule Inhibitors in Golgi Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Bui,Drew Stark,Jie Li,Jianchao Zhang,Yanzhuang Wang
View all publications
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Product promise

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