AB153725

Anti-ARL13B antibody

Name: Anti-ARL13B antibody (ab153725) | Abcam
Brand: Abcam Limited
SKU: AB153725
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal ARL13B antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARL13B aa 100 to C-terminus.

View Alternative Names

ARL2L1, ARL13B, ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2-like protein 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL13B antibody (AB153725)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL13B antibody (AB153725)

Immunohistochemical analysis of paraffin-embedded U87 xenograft, labeling ARL13B using ab153725 at 1/500 dilution.

Supplier Data

Western blot - Anti-ARL13B antibody (AB153725)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-ARL13B antibody (ab153725) at 1/1000 dilution

Lane 1:

RMS-13 whole cell extracts at 30 µg

Lane 2:

RT-4 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-ARL13B antibody (AB153725)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ARL13B antibody (ab153725) at 1/10000 dilution

All lanes:

Human plasma at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 48 kDa

false

Unknown

Western blot - Anti-ARL13B antibody (AB153725)

10% SDS PAGE

All lanes:

Western blot - Anti-ARL13B antibody (ab153725) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate at 50 µg

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ARL13B aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q3SXY8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARL13B also known as ADP-ribosylation factor-like protein 13B is a small GTPase with a molecular mass of approximately 45 kDa. This protein predominantly localizes to the ciliary membrane of cells a specific structure important for cell signaling in eukaryotic organisms. Beyond its association with the cilia ARL13B expression is notable in complex neuronal tissues and renal epithelial cells indicating its diverse roles in various physiological processes.

Biological function summary

ARL13B plays an essential role in the formation and maintenance of primary cilia which are key to cell signaling and sensory processing. ARL13B functions as part of a multiprotein complex within the ciliary membrane essential for the localization and distribution of other ciliary proteins. Through its GTPase activity ARL13B regulates ciliary dynamics and signaling pathways that are fundamental to proper cellular function and development.

Pathways

ARL13B modulates the Sonic Hedgehog (Shh) and Wnt signaling pathways both critical in developmental processes and tissue homeostasis. ARL13B interacts with several proteins in these pathways to control signal transduction such as Gli proteins in the Shh pathway and various cytoplasmic and nuclear factors in the Wnt pathway. ARL13B's role as a signaling regulator highlights its importance in maintaining cellular communication and function.

Mutations or dysregulation of ARL13B are associated with Joubert syndrome and Meckel-Gruber syndrome both developmental disorders affecting the brain and other organs. These conditions arise partly from disrupted cilia function linking ARL13B directly to cilia-related syndromes. In Joubert syndrome ARL13B interacts with proteins such as Cep290 and IFT88 emphasizing its involvement in the pathology of ciliary dysfunctions and providing a focal point for understanding and potentially targeting these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. Binds GTP but is not able to hydrolyze fit; the GTPase activity remains unclear. Required to pattern the neural tube. Involved in cerebral cortex development : required for the initial formation of a polarized radial glial scaffold, the first step in the construction of the cerebral cortex, by regulating ciliary signaling. Regulates the migration and placement of postmitotic interneurons in the developing cerebral cortex. Plays a role in ciliar trafficking of phosphatidylinositol phosphatase INPP5E in ciliogenesis (PubMed : 38219074). May regulate ARF6- and RAB22A-dependent endocytic recycling traffic (PubMed : 23223633).

See full target information ARL13B

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1418456 PubMed39104395

2024

Pan-cancer analyses reveal the molecular and clinical characteristics of TET family members and suggests that TET3 maybe a potential therapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Zhang,Jie Zheng,Jin Liu,Yanxia Li,Guoqiang Xing,Shupeng Zhang,Hekai Chen,Jian Wang,Zhijiang Shao,Yongyuan Li,Zhongmin Jiang,Yingzi Pan,Xiaozhi Liu,Ping Xu,Wenhan Wu

Scientific reports 13:6118 PubMed37059819

2023

Modulation of tau tubulin kinases (TTBK1 and TTBK2) impacts ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Frances M Bashore,Ariana B Marquez,Apirat Chaikuad,Stefanie Howell,Andrea S Dunn,Alvaro A Beltran,Jeffery L Smith,David H Drewry,Adriana S Beltran,Alison D Axtman

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARL13B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL13B antibody (AB153725)

Western blot - Anti-ARL13B antibody (AB153725)

Western blot - Anti-ARL13B antibody (AB153725)

Western blot - Anti-ARL13B antibody (AB153725)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise