Anti-ARL13B antibody [N295B/66]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL13B antibody [N295B/66] (AB136648)

IHC image of ARL13B staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab136648, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

Project11231****

Western blot - Anti-ARL13B antibody [N295B/66] (AB136648)

This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab136648 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.

Arl13b protein has been shown to run at approximately 60-kDa. This is due to post-translational modifications (PMID : 17488627).

All lanes:

Western blot - Anti-ARL13B antibody [N295B/66] (ab136648) at 5 µg/mL

All lanes:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 25 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/10000 dilution

Predicted band size: 49 kDa

Observed band size: 60 kDa

false

Exposure time: 20min

• WB

Project11231****

Western blot - Anti-ARL13B antibody [N295B/66] (AB136648)

This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 1 hour 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab136648 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-ARL13B antibody [N295B/66] (ab136648) at 5 µg/mL

Lane 1:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 25 µg

Lane 2:

Mouse Fetus (14 Day Old) Tissue Lysate at 25 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/10000 dilution

Predicted band size: 49 kDa

Observed band size: 60 kDa

false