Rabbit Polyclonal ARL14 antibody. Suitable for IHC-P and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARL14 aa 1 to C-terminus.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARL14 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N4G2
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Predicted |
| Mouse | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
GTPase that recruits MYO1E to MHC class II-containing vesicles via the effector protein ARL14EP and hence controls the movement of these vesicles along the actin cytoskeleton in dendritic cells.
Alternative names
ADP-ribosylation factor-like protein 14, ADP-ribosylation factor 7, ARF7, ARL14
Recommended products
Rabbit Polyclonal ARL14 antibody. Suitable for IHC-P and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARL14 aa 1 to C-terminus.
Alternative names
ADP-ribosylation factor-like protein 14, ADP-ribosylation factor 7, ARF7, ARL14
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARL14 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N4G2
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARL14 also known as ARF-like 14 is a small GTPase belonging to the ARF family. It plays important roles in regulating membrane trafficking and cytoskeletal dynamics. The protein weighs approximately 20 kDa and is mainly expressed in immune cells such as dendritic cells and macrophages. Its expression is particularly high in the spleen and lymph nodes suggestive of its important roles in immune function.
- Biological function summary
ARL14 influences the formation and function of the immunological synapse. It has strong interactions with myosin 1E forming a complex that influences actin-based movement. By modulating the rearrangement of the actin cytoskeleton ARL14 supports important immune synapse activities which are pivotal for antigen presentation and T cell activation. This activity highlights its importance in maintaining efficient immune response.
- Pathways
ARL14 is involved in the Rho GTPase signaling pathway. This pathway regulates a wide range of cellular processes like cell shape motility and cycle progression. ARL14 works alongside proteins such as Rac and Cdc42 which are also part of the Rho family to support cytoskeletal reorganization. Through these pathways ARL14 helps in trafficking immune receptors and signaling complexes to the correct cellular locations thereby assisting innate and adaptive immune responses.
- Associated diseases and disorders
Researchers have linked ARL14 to autoimmune diseases and chronic inflammatory conditions. Dysregulation of ARL14 may contribute to altered immune synapse formation which can lead to improper immune activation seen in conditions like rheumatoid arthritis. Its association with the myosin 1E protein further implicates ARL14 in these disorders as disruptions in actin motility and membrane trafficking can exacerbate disease pathogenesis. Understanding ARL14's specific roles in these conditions might offer new avenues for therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL14 antibody (ab97811)
Immunohistochemical analysis of paraffin-embedded mouse duodenum tissue labelling ARL14 with ab97811 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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