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AB97811

Anti-ARL14 antibody

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(1 Publication)

Rabbit Polyclonal ARL14 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARL14 aa 1 to C-terminus.

View Alternative Names

ARF7, ARL14, ADP-ribosylation factor-like protein 14, ADP-ribosylation factor 7

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL14 antibody (AB97811)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL14 antibody (AB97811)

Immunohistochemical analysis of paraffin-embedded mouse duodenum tissue labelling ARL14 with ab97811 at 1/500 dilution. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ARL14 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8N4G2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARL14 also known as ARF-like 14 is a small GTPase belonging to the ARF family. It plays important roles in regulating membrane trafficking and cytoskeletal dynamics. The protein weighs approximately 20 kDa and is mainly expressed in immune cells such as dendritic cells and macrophages. Its expression is particularly high in the spleen and lymph nodes suggestive of its important roles in immune function.
Biological function summary

ARL14 influences the formation and function of the immunological synapse. It has strong interactions with myosin 1E forming a complex that influences actin-based movement. By modulating the rearrangement of the actin cytoskeleton ARL14 supports important immune synapse activities which are pivotal for antigen presentation and T cell activation. This activity highlights its importance in maintaining efficient immune response.

Pathways

ARL14 is involved in the Rho GTPase signaling pathway. This pathway regulates a wide range of cellular processes like cell shape motility and cycle progression. ARL14 works alongside proteins such as Rac and Cdc42 which are also part of the Rho family to support cytoskeletal reorganization. Through these pathways ARL14 helps in trafficking immune receptors and signaling complexes to the correct cellular locations thereby assisting innate and adaptive immune responses.

Researchers have linked ARL14 to autoimmune diseases and chronic inflammatory conditions. Dysregulation of ARL14 may contribute to altered immune synapse formation which can lead to improper immune activation seen in conditions like rheumatoid arthritis. Its association with the myosin 1E protein further implicates ARL14 in these disorders as disruptions in actin motility and membrane trafficking can exacerbate disease pathogenesis. Understanding ARL14's specific roles in these conditions might offer new avenues for therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase that recruits MYO1E to MHC class II-containing vesicles via the effector protein ARL14EP and hence controls the movement of these vesicles along the actin cytoskeleton in dendritic cells.
See full target information ARL14
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 8: PubMed31741433

2019

Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zeng,Johnny Castillo-Cabrera,Mika Manser,Bo Lu,Zinger Yang,Vaik Strande,Damien Begue,Raffaella Zamponi,Shumei Qiu,Frederic Sigoillot,Qiong Wang,Alicia Lindeman,John S Reece-Hoyes,Carsten Russ,Debora Bonenfant,Xiaomo Jiang,Youzhen Wang,Feng Cong
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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