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AB183510

Anti-ARL2 antibody [EPR14436]

4

(1 Review)

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(9 Publications)

Rabbit Recombinant Monoclonal ARL2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications.

View Alternative Names

ADP-ribosylation factor-like protein 2, ARL2

5 Images
Flow Cytometry (Intracellular) - Anti-ARL2 antibody [EPR14436] (AB183510)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ARL2 antibody [EPR14436] (AB183510)

Intracellular flow cytometrical analysis of T47D cells labeling ARL2 with ab183510 at 1/100 (green) or a rabbit IgG (negative) (red).

Immunocytochemistry/ Immunofluorescence - Anti-ARL2 antibody [EPR14436] (AB183510)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARL2 antibody [EPR14436] (AB183510)

Immunofluorescent analysis of A549 cells labeling ARL2 with ab183510 at 1/250 (red) and DAPI staining (blue).

Western blot - Anti-ARL2 antibody [EPR14436] (AB183510)
  • WB

Supplier Data

Western blot - Anti-ARL2 antibody [EPR14436] (AB183510)

All lanes:

Western blot - Anti-ARL2 antibody [EPR14436] (ab183510) at 1/2000 dilution

All lanes:

U937 (Human histiocytic lymphoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 19 kDa

false

Western blot - Anti-ARL2 antibody [EPR14436] (AB183510)
  • WB

Supplier Data

Western blot - Anti-ARL2 antibody [EPR14436] (AB183510)

All lanes:

Western blot - Anti-ARL2 antibody [EPR14436] (ab183510) at 1/10000 dilution

Lane 1:

T-47D cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 19 kDa

false

Western blot - Anti-ARL2 antibody [EPR14436] (AB183510)
  • WB

Lab

Western blot - Anti-ARL2 antibody [EPR14436] (AB183510)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ARL2 antibody [EPR14436] (ab183510) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

A549 (Human lung carcinoma epithelial cell) transfected with siRNA specifically targeting ARL2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 120s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14436

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

{ 'ARL2' } is a member of the ARF-like protein family and is also known as ADP-ribosylation factor-like protein 2. This small GTP-binding protein has a molecular mass of approximately 22 kDa. Researchers find it expressed in various human tissues including the kidney heart and brain. This wide expression suggests its relevance in multiple cellular functions. Through its involvement in microtubule dynamics ARL2 plays a role in cytoskeletal organization. Its presence in different cellular compartments is linked to diverse functions.
Biological function summary

{ 'ARL2' } is integral to intracellular transport and mitochondrial function. It forms a complex with other proteins such as cofactor D and ARL2BP to influence the stability and assembly of microtubules and assist in the correct folding of tubulin. ARL2 contributes to maintaining proper mitochondrial morphology and energy production. Additionally it participates in regulating apoptosis highlighting its versatility in cellular operations.

Pathways

{ 'ARL2' } functions within the MAPK/ERK signaling pathway which is vital for cell growth and differentiation. It also interacts with the mitochondrial import pathway cooperating with proteins like TOMM7 to regulate mitochondrial protein translocation. These interactions position ARL2 as an important intermediary step in relaying signals vital for cellular homeostasis and energy metabolism.

Researchers have associated { 'ARL2' } with neurodegenerative diseases such as Charcot-Marie-Tooth disease type 2A. This association stems from its interaction with mitofusin 2 a protein critical for mitochondrial fusion. Additionally abnormalities in ARL2 expression have implications for certain cancers as disrupted microtubule dynamics can lead to unchecked cell division. Understanding these associations provides insights into the potential for ARL2 as a therapeutic target in disease treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine. Required for normal progress through the cell cycle (PubMed : 10831612, PubMed : 16525022, PubMed : 18234692, PubMed : 18588884, PubMed : 20740604). Also regulates mitochondrial integrity and function (PubMed : 30945270).
See full target information ADP-ribosylation factor-like protein 2
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 22:e3002751 PubMed39137170

2024

Arl2 GTPase associates with the centrosomal protein Cdk5rap2 to regulate cortical development via microtubule organization.

Applications

Unspecified application

Species

Unspecified reactive species

Dongliang Ma,Kun-Yang Lin,Divya Suresh,Jiaen Lin,Mahekta R Gujar,Htet Yamin Aung,Ye Sing Tan,Yang Gao,Anselm S Vincent,Teng Chen,Hongyan Wang

Chinese medical journal 137:1823-1834 PubMed38973237

2024

Association of copy number variation in X chromosome-linked PNPLA4 with heterotaxy and congenital heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

Han Gao,Xianghui Huang,Weicheng Chen,Zhiyu Feng,Zhengshan Zhao,Ping Li,Chaozhong Tan,Jinxin Wang,Quannan Zhuang,Yuan Gao,Shaojie Min,Qinyu Yao,Maoxiang Qian,Xiaojing Ma,Feizhen Wu,Weili Yan,Wei Sheng,Guoying Huang

Gynecologic and obstetric investigation 88:159-167 PubMed36882022

2023

Expression of HMGB1-TLR4 in Placentas from Preeclamptic Pregnancies and Its Effect on Proliferation and Invasion of HTR-8/SVneo Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lidan He,Qiongfang Song,Jifen Hu,Jianbo Wu

Histology and histopathology 38:571-584 PubMed36300495

2022

Circ_0085616 contributes to the radio-resistance and progression of cervical cancer by targeting miR-541-3p/ARL2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Tang,Lei Zhou,Lili Liu

International journal of molecular sciences 23: PubMed35563042

2022

Identification of Potential Muscle Biomarkers in McArdle Disease: Insights from Muscle Proteome Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Inés García-Consuegra,Sara Asensio-Peña,Rocío Garrido-Moraga,Tomàs Pinós,Cristina Domínguez-González,Alfredo Santalla,Gisela Nogales-Gadea,Pablo Serrano-Lorenzo,Antoni L Andreu,Joaquín Arenas,José L Zugaza,Alejandro Lucia,Miguel A Martín

Blood cancer journal 12:64 PubMed35422065

2022

Validation of a small molecule inhibitor of PDE6D-RAS interaction with favorable anti-leukemic effects.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Canovas Nunes,Serena De Vita,Andrew Anighoro,François Autelitano,Edward Beaumont,Pamela Klingbeil,Meaghan McGuinness,Beatrice Duvert,Chad Harris,Lu Yang,Sheela Pangeni Pokharel,Chun-Wei Chen,Monika Ermann,David A Williams,Haiming Xu

Open life sciences 16:1-13 PubMed33817293

2021

LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Liu,Dongmei Yao,Bo Huang

Cancers 12: PubMed32106628

2020

Reduced Expression Induces Chromosome Instability through Aberrant Cyclin E1 Protein Turnover.

Applications

Unspecified application

Species

Unspecified reactive species

Laura L Thompson,Allison K Baergen,Zelda Lichtensztejn,Kirk J McManus

Clinical science (London, England : 1979) 133:1871-1881 PubMed31434696

2019

Exosomal microRNA-122 mediates obesity-related cardiomyopathy through suppressing mitochondrial ADP-ribosylation factor-like 2.

Applications

Unspecified application

Species

Unspecified reactive species

Yongshun Wang,Pianpian Jin,Jingjin Liu,Xiang Xie
View all publications
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