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AB250664

Anti-ARL2 antibody [EPR14436] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ARL2 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ADP-ribosylation factor-like protein 2, ARL2

5 Images
Flow Cytometry (Intracellular) - Anti-ARL2 antibody [EPR14436] - BSA and Azide free (AB250664)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ARL2 antibody [EPR14436] - BSA and Azide free (AB250664)

This data was developed using ab183510, the same antibody clone in a different buffer formulation.

Intracellular flow cytometrical analysis of T47D cells labeling ARL2 with ab183510 at 1/100 (green) or a rabbit IgG (negative) (red).

Immunocytochemistry/ Immunofluorescence - Anti-ARL2 antibody [EPR14436] - BSA and Azide free (AB250664)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARL2 antibody [EPR14436] - BSA and Azide free (AB250664)

This data was developed using ab183510, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of A549 cells labeling ARL2 with ab183510 at 1/250 (red) and DAPI staining (blue).

Western blot - Anti-ARL2 antibody [EPR14436] - BSA and Azide free (AB250664)
  • WB

Supplier Data

Western blot - Anti-ARL2 antibody [EPR14436] - BSA and Azide free (AB250664)

This data was developed using ab183510, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARL2 antibody [EPR14436] (<a href='/en-us/products/primary-antibodies/arl2-antibody-epr14436-ab183510'>ab183510</a>) at 1/2000 dilution

All lanes:

U937 (Human histiocytic lymphoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 19 kDa

false

Western blot - Anti-ARL2 antibody [EPR14436] - BSA and Azide free (AB250664)
  • WB

Supplier Data

Western blot - Anti-ARL2 antibody [EPR14436] - BSA and Azide free (AB250664)

This data was developed using ab183510, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARL2 antibody [EPR14436] (<a href='/en-us/products/primary-antibodies/arl2-antibody-epr14436-ab183510'>ab183510</a>) at 1/10000 dilution

Lane 1:

T-47D cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 19 kDa

false

Western blot - Anti-ARL2 antibody [EPR14436] - BSA and Azide free (AB250664)
  • WB

Lab

Western blot - Anti-ARL2 antibody [EPR14436] - BSA and Azide free (AB250664)

This data was developed using ab183510, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARL2 antibody [EPR14436] (<a href='/en-us/products/primary-antibodies/arl2-antibody-epr14436-ab183510'>ab183510</a>) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

A549 (Human lung carcinoma epithelial cell) transfected with siRNA specifically targeting ARL2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 120s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14436

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250664 is the carrier-free version of ab183510.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

{ 'ARL2' } is a member of the ARF-like protein family and is also known as ADP-ribosylation factor-like protein 2. This small GTP-binding protein has a molecular mass of approximately 22 kDa. Researchers find it expressed in various human tissues including the kidney heart and brain. This wide expression suggests its relevance in multiple cellular functions. Through its involvement in microtubule dynamics ARL2 plays a role in cytoskeletal organization. Its presence in different cellular compartments is linked to diverse functions.
Biological function summary

{ 'ARL2' } is integral to intracellular transport and mitochondrial function. It forms a complex with other proteins such as cofactor D and ARL2BP to influence the stability and assembly of microtubules and assist in the correct folding of tubulin. ARL2 contributes to maintaining proper mitochondrial morphology and energy production. Additionally it participates in regulating apoptosis highlighting its versatility in cellular operations.

Pathways

{ 'ARL2' } functions within the MAPK/ERK signaling pathway which is vital for cell growth and differentiation. It also interacts with the mitochondrial import pathway cooperating with proteins like TOMM7 to regulate mitochondrial protein translocation. These interactions position ARL2 as an important intermediary step in relaying signals vital for cellular homeostasis and energy metabolism.

Researchers have associated { 'ARL2' } with neurodegenerative diseases such as Charcot-Marie-Tooth disease type 2A. This association stems from its interaction with mitofusin 2 a protein critical for mitochondrial fusion. Additionally abnormalities in ARL2 expression have implications for certain cancers as disrupted microtubule dynamics can lead to unchecked cell division. Understanding these associations provides insights into the potential for ARL2 as a therapeutic target in disease treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine. Required for normal progress through the cell cycle (PubMed : 10831612, PubMed : 16525022, PubMed : 18234692, PubMed : 18588884, PubMed : 20740604). Also regulates mitochondrial integrity and function (PubMed : 30945270).
See full target information ADP-ribosylation factor-like protein 2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 18:20230639 PubMed36820063

2023

Glycoprotein non-metastatic melanoma B interacts with epidermal growth factor receptor to regulate neural stem cell survival and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Yang,Gang Jin,Shihong Chen,Jing Luo,Wei Xu
View all publications
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