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AB188322

Anti-ARL2BP antibody [EPR15265-21]

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(1 Publication)

Rabbit Recombinant Monoclonal ARL2BP antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

BART, BART1, ARL2BP, ADP-ribosylation factor-like protein 2-binding protein, ARF-like 2-binding protein, ARL2-binding protein, Binder of ARF2 protein 1

8 Images
Flow Cytometry (Intracellular) - Anti-ARL2BP antibody [EPR15265-21] (AB188322)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ARL2BP antibody [EPR15265-21] (AB188322)

Intracellular Flow Cytometry analysis of A549 (human lung carcinoma) cells labelling ARL2BP (red) with purified ab188322 at a dilution of 1/200. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-ARL2BP antibody [EPR15265-21] (AB188322)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARL2BP antibody [EPR15265-21] (AB188322)

Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling ARL2BP with ab188322 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counterstained with Dapi (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] (AB188322)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] (AB188322)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ARL2BP with ab188322 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-ARL2BP antibody [EPR15265-21] (AB188322)
  • IP

Supplier Data

Immunoprecipitation - Anti-ARL2BP antibody [EPR15265-21] (AB188322)

Western blot analysis of ARL2BP in HepG2 cell lysate immunoprecipitated with ab188322 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of HepG2 lysate.

Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-ARL2BP antibody [EPR15265-21] (ab188322)

Predicted band size: 19 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] (AB188322)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] (AB188322)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling ARL2BP with ab188322 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-ARL2BP antibody [EPR15265-21] (AB188322)
  • WB

Lab

Western blot - Anti-ARL2BP antibody [EPR15265-21] (AB188322)

Lanes 1-4 : Merged signal (red and green). Green - ab188322 observed at 20 kDa. Red - loading control ab8245 observed at 36 kDa.

ab188322 Anti-ARL2BP antibody [EPR15265-21] was shown to specifically react with ARL2BP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265269 (knockout cell lysate ab258312) was used. Wild-type and ARL2BP knockout samples were subjected to SDS-PAGE. ab188322 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ARL2BP knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ARL2BP knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-arl2bp-knockout-hela-cell-line-ab265269'>ab265269</a>)

Lane 3:

HUVEC cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 19 kDa

Observed band size: 20 kDa

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Western blot - Anti-ARL2BP antibody [EPR15265-21] (AB188322)
  • WB

Supplier Data

Western blot - Anti-ARL2BP antibody [EPR15265-21] (AB188322)

All lanes:

Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322) at 1/10000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

HUVEC cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

Western blot - Anti-ARL2BP antibody [EPR15265-21] (AB188322)
  • WB

Supplier Data

Western blot - Anti-ARL2BP antibody [EPR15265-21] (AB188322)

All lanes:

Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Rat spleen tissue lysate at 10 µg

Lane 4:

RAW 264.7 cell lysate at 10 µg

Lane 5:

NIH/3T3 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

  • Carrier free

    Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15265-21

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IP, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB185964

Anti-ARL2BP antibody [EPR15265]

RabMAb|Recombinant|KO Validated

Western blot - Anti-ARL2BP antibody [EPR15265] (AB185964)

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Complementary Products

Assay Kits

AB283981

Human ARL2BP ELISA Kit

Sandwich ELISA - Human ARL2BP ELISA Kit (AB283981)

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Primary Antibodies

AB183510

Anti-ARL2 antibody [EPR14436]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ARL2 antibody [EPR14436] (AB183510)

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Proteins & Peptides

AB101067

Recombinant Human ARL2BP protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human ARL2BP protein (His tag N-Terminus) (AB101067)

  • 0
  • 0
Assay Kits

AB283981

Human ARL2BP ELISA Kit

Sandwich ELISA - Human ARL2BP ELISA Kit (AB283981)

  • 0
  • 0
Primary Antibodies

AB30682

Anti-SREBP2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB30682)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARL2BP also known as ADP-ribosylation factor-like 2 binding protein plays an important role in cellular transport mechanisms. This protein weighs approximately 17 kDa. ARL2BP is mainly expressed in tissues like heart skeletal muscle and pancreas. It interacts with microtubules and is involved in intracellular trafficking and cell movement.
Biological function summary

ARL2BP is involved in processes maintaining mitochondrial function. It forms part of a protein complex that includes ARL2 a small GTPase which regulates the stability and functionality of tubulin cofactor E (TBCE). This interaction is important for proper microtubule dynamics and may influence effective protein folding within mitochondria.

Pathways

ARL2BP has important roles in the microtubule and mitochondrial maintenance pathways. It interacts significantly with tubulin and the microtubule assembly connecting to pathways governing cell structure and movement. Additionally ARL2BP's function ties closely with the maintenance of mitochondrial health impacting related proteins like BBS1 and BBS4 in the context of mitochondrial homoeostasis.

Disruptions in ARL2BP functions relate to neurological conditions like retinitis pigmentosa. Altered ARL2BP interactions affect proteins such as ARL2 and TBCE which contribute to disease pathology. Additionally these alterations may correlate with metabolic syndromes due to mitochondrial dysregulation linking ARL2BP to broader metabolic and neurodegenerative diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. May play a role as an effector of ARL2.
See full target information ARL2BP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:87 PubMed36750554

2023

p53 promotes peroxisomal fatty acid β-oxidation to repress purine biosynthesis and mediate tumor suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhong Zhao,Xiaojun Zhou,Baoxiang Chen,Mingzhu Lu,Genxin Wang,Nagarajan Elumalai,Chenhui Tian,Jinmiao Zhang,Yanliang Liu,Zhiqiang Chen,Xinyi Zhou,Mingzhi Wu,Mengjiao Li,Edward V Prochownik,Ali Tavassoli,Congqing Jiang,Youjun Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com