Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (AB250951)

This data was developed using ab188322, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of A549 (human lung carcinoma) cells labelling ARL2BP (red) with purified ab188322 at a dilution of 1/200. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (AB250951)

This data was developed using ab188322, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling ARL2BP with ab188322 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counterstained with Dapi (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (AB250951)

This data was developed using ab188322, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ARL2BP with ab188322 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (AB250951)

This data was developed using ab188322, the same antibody clone in a different buffer formulation.

Western blot analysis of ARL2BP in HepG2 cell lysate immunoprecipitated with ab188322 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of HepG2 lysate.

Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-ARL2BP antibody [EPR15265-21] (<a href='/en-us/products/primary-antibodies/arl2bp-antibody-epr15265-21-ab188322'>ab188322</a>)

Predicted band size: 19 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (AB250951)

This data was developed using ab188322, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling ARL2BP with ab188322 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (AB250951)

This data was developed using ab188322, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab188322 observed at 20 kDa. Red - loading control ab8245 observed at 36 kDa.

ab188322 Anti-ARL2BP antibody [EPR15265-21] was shown to specifically react with ARL2BP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265269 (knockout cell lysate ab258312) was used. Wild-type and ARL2BP knockout samples were subjected to SDS-PAGE. ab188322 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ARL2BP antibody [EPR15265-21] (<a href='/en-us/products/primary-antibodies/arl2bp-antibody-epr15265-21-ab188322'>ab188322</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ARL2BP knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ARL2BP knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-arl2bp-knockout-hela-cell-line-ab265269'>ab265269</a>)

Lane 3:

HUVEC cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 19 kDa

Observed band size: 20 kDa

false

• WB

Supplier Data

Western blot - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (AB250951)

This data was developed using ab188322, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARL2BP antibody [EPR15265-21] (<a href='/en-us/products/primary-antibodies/arl2bp-antibody-epr15265-21-ab188322'>ab188322</a>) at 1/10000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

HUVEC cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

• WB

Supplier Data

Western blot - Anti-ARL2BP antibody [EPR15265-21] - BSA and Azide free (AB250951)

This data was developed using ab188322, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ARL2BP antibody [EPR15265-21] (<a href='/en-us/products/primary-antibodies/arl2bp-antibody-epr15265-21-ab188322'>ab188322</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Rat spleen tissue lysate at 10 µg

Lane 4:

RAW 264.7 cell lysate at 10 µg

Lane 5:

NIH/3T3 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false