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AB185964

Anti-ARL2BP antibody [EPR15265]

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(1 Publication)

Rabbit Recombinant Monoclonal ARL2BP antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BART, BART1, ARL2BP, ADP-ribosylation factor-like protein 2-binding protein, ARF-like 2-binding protein, ARL2-binding protein, Binder of ARF2 protein 1

2 Images
Western blot - Anti-ARL2BP antibody [EPR15265] (AB185964)
  • WB

Supplier Data

Western blot - Anti-ARL2BP antibody [EPR15265] (AB185964)

All lanes:

Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964) at 1/2000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

HUVEC lysate at 20 µg

Lane 3:

A549 lysate at 20 µg

Lane 4:

HepG2 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 19 kDa

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Western blot - Anti-ARL2BP antibody [EPR15265] (AB185964)
  • WB

Lab

Western blot - Anti-ARL2BP antibody [EPR15265] (AB185964)

Lanes 1-4 : Merged signal (red and green). Green - ab185964 observed at 20 kDa. Red - loading control ab8245 observed at 36 kDa.

ab185964 Anti-ARL2BP antibody [EPR15265] was shown to specifically react with ARL2BP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265269 (knockout cell lysate ab258312) was used. Wild-type and ARL2BP knockout samples were subjected to SDS-PAGE. ab185964 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ARL2BP knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ARL2BP knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-arl2bp-knockout-hela-cell-line-ab265269'>ab265269</a>)

Lane 3:

HUVEC cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 19 kDa

Observed band size: 20 kDa

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  • Carrier free

    Anti-ARL2BP antibody [EPR15265] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15265

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Cell Lines & Lysates

AB258312

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Western blot - Human ARL2BP knockout HeLa cell lysate (AB258312)

  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARL2BP also known as ADP-ribosylation factor-like 2 binding protein plays an important role in cellular transport mechanisms. This protein weighs approximately 17 kDa. ARL2BP is mainly expressed in tissues like heart skeletal muscle and pancreas. It interacts with microtubules and is involved in intracellular trafficking and cell movement.
Biological function summary

ARL2BP is involved in processes maintaining mitochondrial function. It forms part of a protein complex that includes ARL2 a small GTPase which regulates the stability and functionality of tubulin cofactor E (TBCE). This interaction is important for proper microtubule dynamics and may influence effective protein folding within mitochondria.

Pathways

ARL2BP has important roles in the microtubule and mitochondrial maintenance pathways. It interacts significantly with tubulin and the microtubule assembly connecting to pathways governing cell structure and movement. Additionally ARL2BP's function ties closely with the maintenance of mitochondrial health impacting related proteins like BBS1 and BBS4 in the context of mitochondrial homoeostasis.

Disruptions in ARL2BP functions relate to neurological conditions like retinitis pigmentosa. Altered ARL2BP interactions affect proteins such as ARL2 and TBCE which contribute to disease pathology. Additionally these alterations may correlate with metabolic syndromes due to mitochondrial dysregulation linking ARL2BP to broader metabolic and neurodegenerative diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. May play a role as an effector of ARL2.
See full target information ARL2BP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34884786

2021

Truncation-Driven Lateral Association of α-Synuclein Hinders Amyloid Clearance by the Hsp70-Based Disaggregase.

Applications

Unspecified application

Species

Unspecified reactive species

Aitor Franco,Jorge Cuéllar,José Ángel Fernández-Higuero,Igor de la Arada,Natalia Orozco,José M Valpuesta,Adelina Prado,Arturo Muga
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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