Rabbit Recombinant Monoclonal ARL2BP antibody. Suitable for WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | |
---|---|
Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
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Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. May play a role as an effector of ARL2.
BART, BART1, BART1, ARL2BP, BART, ADP-ribosylation factor-like protein 2-binding protein, ARF-like 2-binding protein, ARL2-binding protein, Binder of ARF2 protein 1
Rabbit Recombinant Monoclonal ARL2BP antibody. Suitable for WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR15265
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
ARL2BP also known as ADP-ribosylation factor-like 2 binding protein plays an important role in cellular transport mechanisms. This protein weighs approximately 17 kDa. ARL2BP is mainly expressed in tissues like heart skeletal muscle and pancreas. It interacts with microtubules and is involved in intracellular trafficking and cell movement.
ARL2BP is involved in processes maintaining mitochondrial function. It forms part of a protein complex that includes ARL2 a small GTPase which regulates the stability and functionality of tubulin cofactor E (TBCE). This interaction is important for proper microtubule dynamics and may influence effective protein folding within mitochondria.
ARL2BP has important roles in the microtubule and mitochondrial maintenance pathways. It interacts significantly with tubulin and the microtubule assembly connecting to pathways governing cell structure and movement. Additionally ARL2BP's function ties closely with the maintenance of mitochondrial health impacting related proteins like BBS1 and BBS4 in the context of mitochondrial homoeostasis.
Disruptions in ARL2BP functions relate to neurological conditions like retinitis pigmentosa. Altered ARL2BP interactions affect proteins such as ARL2 and TBCE which contribute to disease pathology. Additionally these alterations may correlate with metabolic syndromes due to mitochondrial dysregulation linking ARL2BP to broader metabolic and neurodegenerative diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Lanes 1-4: Merged signal (red and green). Green - ab185964 observed at 20 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
ab185964 Anti-ARL2BP antibody [EPR15265] was shown to specifically react with ARL2BP in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human ARL2BP knockout HeLa cell line ab265269 (knockout cell lysate Human ARL2BP knockout HeLa cell lysate ab258312) was used. Wild-type and ARL2BP knockout samples were subjected to SDS-PAGE. ab185964 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: ARL2BP knockout HeLa cell lysate at 20 µg
Lane 3: HUVEC cell lysate at 20 µg
Lane 4: MCF7 cell lysate at 20 µg
All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 19 kDa
Observed band size: 20 kDa
This data was developed using ab185964, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - ab185964 observed at 20 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
ab185964 Anti-ARL2BP antibody [EPR15265] was shown to specifically react with ARL2BP in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human ARL2BP knockout HeLa cell line ab265269 (knockout cell lysate Human ARL2BP knockout HeLa cell lysate ab258312) was used. Wild-type and ARL2BP knockout samples were subjected to SDS-PAGE. ab185964 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964) at 1/2000 dilution
Lane 1: Human fetal brain lysate at 20 µg
Lane 2: HUVEC lysate at 20 µg
Lane 3: A549 lysate at 20 µg
Lane 4: HepG2 lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 19 kDa
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