Skip to main content

Rabbit Recombinant Monoclonal ARL2BP antibody. Suitable for WB and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-ARL2BP antibody [EPR15265] (AB185964), expandable thumbnail
  • Western blot - Anti-ARL2BP antibody [EPR15265] (AB185964), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/2000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. May play a role as an effector of ARL2.

Alternative names

BART, BART1, ARL2BP, ADP-ribosylation factor-like protein 2-binding protein, ARF-like 2-binding protein, ARL2-binding protein, Binder of ARF2 protein 1

Recommended products

Rabbit Recombinant Monoclonal ARL2BP antibody. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15265
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ARL2BP also known as ADP-ribosylation factor-like 2 binding protein plays an important role in cellular transport mechanisms. This protein weighs approximately 17 kDa. ARL2BP is mainly expressed in tissues like heart skeletal muscle and pancreas. It interacts with microtubules and is involved in intracellular trafficking and cell movement.

Biological function summary

ARL2BP is involved in processes maintaining mitochondrial function. It forms part of a protein complex that includes ARL2 a small GTPase which regulates the stability and functionality of tubulin cofactor E (TBCE). This interaction is important for proper microtubule dynamics and may influence effective protein folding within mitochondria.

Pathways

ARL2BP has important roles in the microtubule and mitochondrial maintenance pathways. It interacts significantly with tubulin and the microtubule assembly connecting to pathways governing cell structure and movement. Additionally ARL2BP's function ties closely with the maintenance of mitochondrial health impacting related proteins like BBS1 and BBS4 in the context of mitochondrial homoeostasis.

Associated diseases and disorders

Disruptions in ARL2BP functions relate to neurological conditions like retinitis pigmentosa. Altered ARL2BP interactions affect proteins such as ARL2 and TBCE which contribute to disease pathology. Additionally these alterations may correlate with metabolic syndromes due to mitochondrial dysregulation linking ARL2BP to broader metabolic and neurodegenerative diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964), expandable thumbnail

    Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964)

    Lanes 1-4: Merged signal (red and green). Green - ab185964 observed at 20 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab185964 Anti-ARL2BP antibody [EPR15265] was shown to specifically react with ARL2BP in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human ARL2BP knockout HeLa cell line ab265269 (knockout cell lysate Human ARL2BP knockout HeLa cell lysate ab258312) was used. Wild-type and ARL2BP knockout samples were subjected to SDS-PAGE. ab185964 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: ARL2BP knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human ARL2BP knockout HeLa cell line (Human ARL2BP knockout HeLa cell line ab265269)

    Lane 3: HUVEC cell lysate at 20 µg

    Lane 4: MCF7 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 19 kDa

    Observed band size: 20 kDa

  • Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964), expandable thumbnail

    Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964)

    All lanes: Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964) at 1/2000 dilution

    Lane 1: Human fetal brain lysate at 20 µg

    Lane 2: HUVEC lysate at 20 µg

    Lane 3: A549 lysate at 20 µg

    Lane 4: HepG2 lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com