Rabbit Recombinant Monoclonal ARL4A antibody. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-ARL4A antibody [EPR6086] (AB124690), expandable thumbnail](https://content.abcam.com/products/images/arl4a-antibody-epr6086-ab124690--western-blot-img91542.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ARL4A antibody [EPR6086] (AB124690)")
![OI-RD Scanning - Anti-ARL4A antibody [EPR6086] (AB124690), expandable thumbnail](https://content.abcam.com/products/images/arl4a-antibody-epr6086-ab124690--oi-rd-scanning-img84281.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-ARL4A antibody [EPR6086] (AB124690)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
1 products for Alternative Version
Target data
Function
Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form.
Alternative names
ADP-ribosylation factor-like protein 4A, ARL4A, ARL4
Recommended products
Rabbit Recombinant Monoclonal ARL4A antibody. Suitable for WB and reacts with Human samples.
Alternative names
ADP-ribosylation factor-like protein 4A, ARL4A, ARL4
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR6086
- Purification technique
Affinity purification Protein A
- Dissociation constant
9.34 x 10-10 M
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- Activity summary
ARL4A (ADP-Ribosylation Factor-Like 4A) acts as a small GTPase in the ADP-ribosylation factor family. This protein also known as ARF-like protein has a mass of approximately 20 kDa. ARL4A is expressed in various tissues including the brain and kidneys. It plays an important role in intracellular signaling impacting cytoskeleton dynamics and membrane trafficking.
- Biological function summary
ARL4A impacts cellular processes through its involvement in actin cytoskeleton reorganization. This makes ARL4A important in cell migration and morphogenesis. As part of the ARF family ARL4A may interact with other small GTPases forming a signaling complex that modulates cellular function. Its activity helps maintain cell structure and movement reflecting its influence on morphogenetic processes.
- Pathways
ARL4A participates in signal transduction networks like the Ras-related pathways. It links to proteins such as ARF6 showing its involvement in vesicular trafficking and cytoskeleton remodeling. Through these pathways ARL4A helps coordinate intracellular activities that maintain cell shape and adhesion contributing to overall cellular dynamics.
- Associated diseases and disorders
ARL4A links to pathological conditions like cancer and metabolic disorders. Abnormal ARL4A expression associates with oncogenic processes suggesting its influence on tumorigenesis. Additionally disruptions in ARL4A function might involve interactions with proteins like ARL6 indicating potential metabolic dysregulation. These connections highlight ARL4A's significance in disease development and progression marking it as an important target in disease research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-ARL4A antibody [EPR6086] (ab124690), expandable thumbnail](https://content.abcam.com/products/images/arl4a-antibody-epr6086-ab124690--western-blot-img91542.jpg "Western blot - Anti-ARL4A antibody [EPR6086] (ab124690)")
Western blot - Anti-ARL4A antibody [EPR6086] (ab124690)
We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
Blocking/Diluting Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-ARL4A antibody [EPR6086] (ab124690) at 1/1000 dilution
All lanes: Human small intestine tissue lysate prepared in 1% SDS Hot lysis method at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
![OI-RD Scanning - Anti-ARL4A antibody [EPR6086] (ab124690), expandable thumbnail](https://content.abcam.com/products/images/arl4a-antibody-epr6086-ab124690--oi-rd-scanning-img84281.jpg "OI-RD Scanning - Anti-ARL4A antibody [EPR6086] (ab124690)")
OI-RD Scanning - Anti-ARL4A antibody [EPR6086] (ab124690)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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