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AB247961

Anti-ARL4A antibody [EPR6086] - BSA and Azide free

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Rabbit Recombinant Monoclonal ARL4A antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

ARL4, ARL4A, ADP-ribosylation factor-like protein 4A

2 Images
Western blot - Anti-ARL4A antibody [EPR6086] - BSA and Azide free (AB247961)
  • WB

Lab

Western blot - Anti-ARL4A antibody [EPR6086] - BSA and Azide free (AB247961)

This data was developed using ab124690, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

All lanes:

Western blot - Anti-ARL4A antibody [EPR6086] (<a href='/en-us/products/primary-antibodies/arl4a-antibody-epr6086-ab124690'>ab124690</a>) at 1/1000 dilution

All lanes:

Human small intestine tissue lysate prepared in 1% SDS Hot lysis method at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

OI-RD Scanning - Anti-ARL4A antibody [EPR6086] - BSA and Azide free (AB247961)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ARL4A antibody [EPR6086] - BSA and Azide free (AB247961)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6086

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247961 is the carrier-free version of ab124690.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARL4A (ADP-Ribosylation Factor-Like 4A) acts as a small GTPase in the ADP-ribosylation factor family. This protein also known as ARF-like protein has a mass of approximately 20 kDa. ARL4A is expressed in various tissues including the brain and kidneys. It plays an important role in intracellular signaling impacting cytoskeleton dynamics and membrane trafficking.
Biological function summary

ARL4A impacts cellular processes through its involvement in actin cytoskeleton reorganization. This makes ARL4A important in cell migration and morphogenesis. As part of the ARF family ARL4A may interact with other small GTPases forming a signaling complex that modulates cellular function. Its activity helps maintain cell structure and movement reflecting its influence on morphogenetic processes.

Pathways

ARL4A participates in signal transduction networks like the Ras-related pathways. It links to proteins such as ARF6 showing its involvement in vesicular trafficking and cytoskeleton remodeling. Through these pathways ARL4A helps coordinate intracellular activities that maintain cell shape and adhesion contributing to overall cellular dynamics.

ARL4A links to pathological conditions like cancer and metabolic disorders. Abnormal ARL4A expression associates with oncogenic processes suggesting its influence on tumorigenesis. Additionally disruptions in ARL4A function might involve interactions with proteins like ARL6 indicating potential metabolic dysregulation. These connections highlight ARL4A's significance in disease development and progression marking it as an important target in disease research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form.
See full target information ARL4A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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