Rabbit Polyclonal ARL4C antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARL4C aa 50 to C-terminus.
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Publications
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- Cell cycle (Georgetown, Tex.) 21:392-4052022Identifying a novel IRF3/circUHRF1/miR-1306-5p/ARL4C axis in pancreatic ductal adenocarcinoma progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Liu,Lisha Deng,Anchun Xu,Xingcheng Xiong,Jing Tao,Jian Chang,Yiling Xu,Zhilin ZhouPubMed 34983293
- OncoTargets and therapy 14:2541-25532021MicroRNA-302s Might Regulate ARL4C-Mediated Gastric Cancer Progression via p53 Signaling: Bioinformatics Analysis and Experiments Validation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNing Xie,Yifei Pan,Jian Wu,Yunfan Bai,Cailan Xiao,Xiaoliang Gao,Jinhai Wang,Na LiuPubMed 33880033
- Journal of Cancer 12:818-8262021ADP-Ribosylation Factor Like GTPase 4C (ARL4C) augments stem-like traits of glioblastoma cells by upregulating ALDH1A3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Chen et. al.PubMed 33403039
- Frontiers in oncology 10:5852922020The Role of in Erlotinib Resistance: Activation of the //β- Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinrong Liao et. al.PubMed 33194732
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARL4C aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P56559
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. May be involved in transport between a perinuclear compartment and the plasma membrane, apparently linked to the ABCA1-mediated cholesterol secretion pathway. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in the GDP-bound form. Regulates the microtubule-dependent intracellular vesicular transport from early endosome to recycling endosome process.
Alternative names
ARL7, ARL4C, ADP-ribosylation factor-like protein 4C, ADP-ribosylation factor-like protein 7, ADP-ribosylation factor-like protein LAK
Recommended products
Rabbit Polyclonal ARL4C antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARL4C aa 50 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARL4C aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P56559
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ARL4C protein also known as ADP-ribosylation factor-like 4C is a small GTPase member of the ARF family with a molecular mass of approximately 20-25 kDa. This protein plays roles in regulating cytoskeletal organization and intracellular trafficking. ARL4C is expressed in various tissues including the brain kidney and liver where it contributes to cellular processes. Its expression levels can vary depending on the physiological conditions and requirements of specific tissues.
- Biological function summary
ARL4C contributes to cellular proliferation and migration. It often associates with plasma membranes and cytosolic complexes participating in the regulation of cytoskeletal dynamics through its interaction with other actin-modulating proteins. Additionally ARL4C modulates the activity of signaling pathways that involve small GTPases exerting influence over cell shape and movement which are important for maintaining cellular structure and facilitating response to external stimuli.
- Pathways
ARL4C plays an integrating role in the Wnt and MAPK signaling pathways. In the Wnt signaling pathway ARL4C interacts with proteins such as Dishevelled influencing cell fate determination and embryonic development. In the MAPK pathway it has connections that affect cellular responses to growth factors and stress signals interacting with kinases like ERK1/2 which modulate cell division and survival. The involvement of ARL4C in these pathways highlights its importance in regulating diverse biological responses.
- Associated diseases and disorders
ARL4C has been linked to cancer and fibrosis. Overexpression of ARL4C has been observed in several cancers where it promotes tumor cell migration and invasion suggesting a potential role in cancer progression. In pulmonary fibrosis altered ARL4C expression affects the fibrotic process influencing tissue repair mechanisms. In both cases interactions with proteins such as Rac1 and other family GTPases are critical as they modulate various cellular processes related to disease development and progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL4C antibody (ab122025)
ab122025 at 1/120 dilution staining ARL4C in paraffin-embedded Human skeletal muscle by Immunohistochemistry.
Western blot - Anti-ARL4C antibody (ab122025)
All lanes: Western blot - Anti-ARL4C antibody (ab122025) at 1/250 dilution
Lane 1: RT-4 cell lysate.
Lane 2: U-251 MG cell lysate.
Lane 3: Human plasma
Lane 4: Human Liver tissue lysate.
Lane 5: Human Tonsil tissue lysate.
Developed using the ECL technique.
Predicted band size: 21 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ARL4C antibody (ab122025)
ab122025 at 1 μg/ml staining ARL4C (green) in PFA-fixed, Triton X-100 permeabilized, A-431 cells by Immunofluorescence.
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