JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB122025

Anti-ARL4C antibody

Be the first to review this product! Submit a review

|

(6 Publications)

Rabbit Polyclonal ARL4C antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARL4C aa 50 to C-terminus.

View Alternative Names

ARL7, ARL4C, ADP-ribosylation factor-like protein 4C, ADP-ribosylation factor-like protein 7, ADP-ribosylation factor-like protein LAK

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL4C antibody (AB122025)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL4C antibody (AB122025)

ab122025 at 1/120 dilution staining ARL4C in paraffin-embedded Human skeletal muscle by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-ARL4C antibody (AB122025)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ARL4C antibody (AB122025)

ab122025 at 1 μg/ml staining ARL4C (green) in PFA-fixed, Triton X-100 permeabilized, A-431 cells by Immunofluorescence.

Western blot - Anti-ARL4C antibody (AB122025)
  • WB

Unknown

Western blot - Anti-ARL4C antibody (AB122025)

All lanes:

Western blot - Anti-ARL4C antibody (ab122025) at 1/250 dilution

Lane 1:

RT-4 cell lysate.

Lane 2:

U-251 MG cell lysate.

Lane 3:

Human plasma

Lane 4:

Human Liver tissue lysate.

Lane 5:

Human Tonsil tissue lysate.

Predicted band size: 21 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ARL4C aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P56559

style
left-column

Complementary Products

Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Primary Antibodies

AB203829

Anti-ZEB1 antibody [EPR17375]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-ZEB1 antibody [EPR17375] (AB203829)

  • 3
  • 3
Sample Prep & Detection Kits

AB64238

DAB Substrate Kit

Immunohistochemistry - DAB Substrate Kit (AB64238)

  • 5
  • 7
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ARL4C protein also known as ADP-ribosylation factor-like 4C is a small GTPase member of the ARF family with a molecular mass of approximately 20-25 kDa. This protein plays roles in regulating cytoskeletal organization and intracellular trafficking. ARL4C is expressed in various tissues including the brain kidney and liver where it contributes to cellular processes. Its expression levels can vary depending on the physiological conditions and requirements of specific tissues.
Biological function summary

ARL4C contributes to cellular proliferation and migration. It often associates with plasma membranes and cytosolic complexes participating in the regulation of cytoskeletal dynamics through its interaction with other actin-modulating proteins. Additionally ARL4C modulates the activity of signaling pathways that involve small GTPases exerting influence over cell shape and movement which are important for maintaining cellular structure and facilitating response to external stimuli.

Pathways

ARL4C plays an integrating role in the Wnt and MAPK signaling pathways. In the Wnt signaling pathway ARL4C interacts with proteins such as Dishevelled influencing cell fate determination and embryonic development. In the MAPK pathway it has connections that affect cellular responses to growth factors and stress signals interacting with kinases like ERK1/2 which modulate cell division and survival. The involvement of ARL4C in these pathways highlights its importance in regulating diverse biological responses.

ARL4C has been linked to cancer and fibrosis. Overexpression of ARL4C has been observed in several cancers where it promotes tumor cell migration and invasion suggesting a potential role in cancer progression. In pulmonary fibrosis altered ARL4C expression affects the fibrotic process influencing tissue repair mechanisms. In both cases interactions with proteins such as Rac1 and other family GTPases are critical as they modulate various cellular processes related to disease development and progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. May be involved in transport between a perinuclear compartment and the plasma membrane, apparently linked to the ABCA1-mediated cholesterol secretion pathway. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in the GDP-bound form. Regulates the microtubule-dependent intracellular vesicular transport from early endosome to recycling endosome process.
See full target information ARL4C
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 51: PubMed38131251

2023

Ursolic acid inhibits the metastasis of colon cancer by downregulating ARL4C expression.

Applications

Unspecified application

Species

Unspecified reactive species

Mengzhe Zhang,Fenfen Xiang,Yipeng Sun,Rongrong Liu,Qian Li,Qing Gu,Xiangdong Kang,Rong Wu

Journal of oncology 2022:2724515 PubMed35774359

2022

ARL4C Regulates the Progression of Clear Cell Renal Cell Carcinoma by Affecting the Wnt/-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peizhi Zhang,Yingkun Xu,Shaoan Chen,Zicheng Wang,Leizuo Zhao,Chen Chen,Weiting Kang,Rongyu Han,Jiechuan Qiu,Qingliang Wang,Han Gao,Guangzhen Wu,Qinghua Xia

Cell cycle (Georgetown, Tex.) 21:392-405 PubMed34983293

2022

Identifying a novel IRF3/circUHRF1/miR-1306-5p/ARL4C axis in pancreatic ductal adenocarcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Lisha Deng,Anchun Xu,Xingcheng Xiong,Jing Tao,Jian Chang,Yiling Xu,Zhilin Zhou

OncoTargets and therapy 14:2541-2553 PubMed33880033

2021

MicroRNA-302s Might Regulate ARL4C-Mediated Gastric Cancer Progression via p53 Signaling: Bioinformatics Analysis and Experiments Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Xie,Yifei Pan,Jian Wu,Yunfan Bai,Cailan Xiao,Xiaoliang Gao,Jinhai Wang,Na Liu

Journal of Cancer 12:818-826 PubMed33403039

2021

ADP-Ribosylation Factor Like GTPase 4C (ARL4C) augments stem-like traits of glioblastoma cells by upregulating ALDH1A3.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Chen,Wen-Juan Fu,Xiao-Peng Tang,Lu Wang,Qin Niu,Shuai Wang,Yong Lin,Mian-Fu Cao,Rong Hu,Hai-Yan Wen,Yan Wang,Xia Zhang,Xiao-Hong Yao

Frontiers in oncology 10:585292 PubMed33194732

2020

The Role of in Erlotinib Resistance: Activation of the //β- Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinrong Liao,Zeng Chen,Zongyang Yu,Tao Huang,Dan Hu,Ying Su,Zhiyong He,Changyan Zou,Lurong Zhang,Xiandong Lin
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com