AB224825

Anti-ARL6IP2 antibody

Name: Anti-ARL6IP2 antibody (ab224825) | Abcam
Brand: Abcam Limited
SKU: AB224825

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(2 Publications)

Rabbit Polyclonal ARL6IP2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ATL2 aa 400 to C-terminus.

View Alternative Names

ARL6IP2, ATL2, Atlastin-2, ATL-2, ADP-ribosylation factor-like protein 6-interacting protein 2

2 Images

Supplier Data

Immunoprecipitation - Anti-ARL6IP2 antibody (AB224825)

ARL6IP2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224825 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab224825 at 0.4 μg/ml.

Lane 1 : ab224825 IP in Jurkat whole cell lysate.

Lane 2 : Control IgG IP in Jurkat whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ARL6IP2 antibody (ab224825)

Predicted band size: 66 kDa

false

Supplier Data

Western blot - Anti-ARL6IP2 antibody (AB224825)

All lanes:

Western blot - Anti-ARL6IP2 antibody (ab224825) at 0.04 µg/mL

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Predicted band size: 66 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ATL2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NHH9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARL6IP2 also known as ADP-ribosylation-like factor 6 interacting protein 2 is a protein involved in various cellular processes. It has a mass of approximately 25 kDa and is expressed in the endoplasmic reticulum. This protein interacts with lipid-raft-associated proteins playing an important role in membrane dynamics and vesicular trafficking. Researchers have observed it in tissues that exhibit high secretory activity which suggests a function associated with protein transport or modification processes within the cell.

Biological function summary

ARL6IP2 interacts with components of the endoplasmic reticulum to maintain cellular homeostasis and structural integrity. It may be part of larger protein complexes involved in intracellular transport. This protein aids in regulating vesicle formation and trafficking which are essential for efficient cellular communication and nutrient delivery. Its ability to reorganize membrane components allows cells to respond to environmental changes effectively facilitating adaptive responses and cellular survival.

Pathways

ARL6IP2 plays a role in the endocytic and secretory pathways. It works alongside other proteins like ARF proteins and clathrin orchestrating trafficking processes that modulate membrane composition and vesicle transport. These pathways are important for normal cellular functioning and involve coordinated interactions with multiple proteins to maintain cellular equilibrium. ARL6IP2's role in these pathways exemplifies its importance in cellular dynamics and intracellular signaling.

Studies suggest links between ARL6IP2 and certain neurodegenerative conditions and cancer. Research indicates alterations in ARL6IP2 expression levels might contribute to the progression of diseases like Alzheimer's due to its involvement in cellular trafficking and membrane stability. The protein's connection to disorders often involves interactions with other proteins such as beta-amyloid precursor protein in Alzheimer's indicating its roles in disease mechanisms and pathogenesis. Understanding these interactions provides insights into developing strategies for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Atlastin-2 (ATL2) is a membrane-anchored GTPase that mediates the GTP-dependent fusion of endoplasmic reticulum (ER) membranes, maintaining the continuous ER network. It facilitates the formation of three-way junctions where ER tubules intersect (PubMed : 18270207, PubMed : 19665976, PubMed : 22065636, PubMed : 27619977, PubMed : 34817557). Two atlastin-2 on neighboring ER tubules bind GTP and form loose homodimers through the GB1/RHD3-type G domains and 3HB regions. Upon GTP hydrolysis, the 3HB regions tighten, pulling the membranes together to drive their fusion. After fusion, the homodimer disassembles upon release of inorganic phosphate (Pi). Subsequently, GDP dissociates, resetting the monomers to a conformation ready for a new fusion cycle (By similarity).

See full target information ATL2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114214 PubMed38761375

2024

TDP1 mutation causing SCAN1 neurodegenerative syndrome hampers the repair of transcriptional DNA double-strand breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Mathéa Geraud,Agnese Cristini,Simona Salimbeni,Nicolas Bery,Virginie Jouffret,Marco Russo,Andrea Carla Ajello,Lara Fernandez Martinez,Jessica Marinello,Pierre Cordelier,Didier Trouche,Gilles Favre,Estelle Nicolas,Giovanni Capranico,Olivier Sordet

Archives of rheumatology 38:119-128 PubMed37235116

2023

MiR-30e-5p deficiency exerts an inhibitory effect on inflammation in rheumatoid arthritis via regulating Atl2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Liu,Kai Wang,Ju Li,Yan Liu,Zhongyuan Zhang,Deqian Meng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ARL6IP2 antibody

Immunoprecipitation - Anti-ARL6IP2 antibody (AB224825)

Western blot - Anti-ARL6IP2 antibody (AB224825)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise