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Rabbit Recombinant Monoclonal ARL8A antibody. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Mouse, Human, Rat samples.

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Images

Western blot - Anti-ARL8A antibody [EPR24376-2] (AB270979), expandable thumbnail
  • Immunoprecipitation - Anti-ARL8A antibody [EPR24376-2] (AB270979), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL8A antibody [EPR24376-2] (AB270979), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ARL8A antibody [EPR24376-2] (AB270979), expandable thumbnail
  • Western blot - Anti-ARL8A antibody [EPR24376-2] (AB270979), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-FrFlow Cyt (Intra)IHC-P
Human
Not recommended
Tested
Tested
Not recommended
Not recommended
Tested
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Tested
Rat
Not recommended
Expected
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Plays a role in lysosome motility (By similarity). In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function (By similarity). May play a role in chromosome segregation (By similarity).

Alternative names

ARL10B, GIE2, ARL8A, ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B, Novel small G protein indispensable for equal chromosome segregation 2

Recommended products

Rabbit Recombinant Monoclonal ARL8A antibody. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Mouse, Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24376-2
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ARL8A also known as Arl8 or ADP-ribosylation factor-like protein 8A is a small GTPase with a molecular mass of approximately 20 kDa. This protein is involved in the regulation of lysosomal positioning and trafficking in the cell. It is expressed in various tissues including the brain liver and lung playing a role in cellular transport mechanisms. ARL8A localizes primarily to the lysosomal membrane contributing to the dynamic behavior of these organelles.

Biological function summary

ARL8A facilitates the proper positioning and movement of lysosomes within the cellular cytoplasm by interacting with the scaffold protein PLEKHM2 and the HOPS complex. These interactions enable the lysosomes to fuse with other vesicles important for maintaining cellular homeostasis and facilitating autophagy. ARL8A's function is particularly important in immune cells where it aids in the degradation of internalized pathogens by enhancing lysosomal fusion capabilities.

Pathways

ARL8A participates in the lysosomal transport and autophagy pathways. This protein functions with other lysosomal proteins like LAMP1 and LAMP2 to ensure the fusion and degradation processes work efficiently. ARL8A also interacts with proteins in the kinesin transport system linking it to the coordination of movement along microtubules impacting intracellular vesicle trafficking significantly.

Associated diseases and disorders

Research links ARL8A to neurodegenerative diseases such as Parkinson's disease where lysosomal dysfunction is a contributing factor. Mutations or misregulation of ARL8A and associated proteins like LRRK2 can lead to impaired autophagy resulting in the accumulation of toxic proteins. Furthermore ARL8A has been connected to immune disorders as its role in lysosomal function can affect the immune response potentially leading to inflammatory diseases if dysregulated.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-ARL8A antibody [EPR24376-2] (ab270979), expandable thumbnail

    Western blot - Anti-ARL8A antibody [EPR24376-2] (ab270979)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-ARL8A antibody [EPR24376-2] (ab270979) at 1/1000 dilution

    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg

    Lane 2: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg

    Lane 3: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 4: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 5: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg

    Lane 6: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 40 µg

    Lane 7: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 40 µg

    Lane 8: C6 (rat glial tumor glial cell) whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Immunoprecipitation - Anti-ARL8A antibody [EPR24376-2] (ab270979), expandable thumbnail

    Immunoprecipitation - Anti-ARL8A antibody [EPR24376-2] (ab270979)

    ARL8A was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 μg with ab270979 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270979 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Neuro-2a whole cell lysate 10 μg

    Lane 2: ab270979 IP in Neuro-2a whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab270979 in Neuro-2a whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 58 seconds

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Immunoprecipitation - Anti-ARL8A antibody [EPR24376-2] (ab270979)

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL8A antibody [EPR24376-2] (ab270979), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL8A antibody [EPR24376-2] (ab270979)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labelling ARL8A with ab270979 at 1/50 (10.02 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab270979 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Cytoplasmic staining on human placenta (PMID: 15331635).

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-ARL8A antibody [EPR24376-2] (ab270979), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ARL8A antibody [EPR24376-2] (ab270979)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a cells labelling ARL8A with ab270979 at 1/50 (10.02 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

    Confocal image showing cytoplasmic staining in Neuro-2a cell line.

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-ARL8A antibody [EPR24376-2] (ab270979), expandable thumbnail

    Western blot - Anti-ARL8A antibody [EPR24376-2] (ab270979)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lanes 1-2: This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-ARL8A antibody [EPR24376-2] (ab270979) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 40 µg

    Lane 2: Human placenta tissue lysate at 40 µg

    Lane 3: Mouse brain tissue lysate at 40 µg

    Lane 4: Mouse placenta tissue lysate at 40 µg

    Lane 5: Rat brain tissue lysate at 40 µg

    Lane 6: Rat placenta tissue lysate at 40 µg

    Secondary

    Lanes 1 - 2: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Lanes 3 - 6: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 21 kDa

  • Western blot - Anti-ARL8A antibody [EPR24376-2] (ab270979), expandable thumbnail

    Western blot - Anti-ARL8A antibody [EPR24376-2] (ab270979)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody reacts with ARL8A, but not ARL8B.

    Exposure time: 3 seconds.

    All lanes: Western blot - Anti-ARL8A antibody [EPR24376-2] (ab270979) at 1/1000 dilution

    Lane 1: His-tagged human ARL8A recombinant protein at 0.02 µg

    Lane 2: His-tagged human ARL8B recombinant protein at 0.02 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa

  • Immunoprecipitation - Anti-ARL8A antibody [EPR24376-2] (ab270979), expandable thumbnail

    Immunoprecipitation - Anti-ARL8A antibody [EPR24376-2] (ab270979)

    ARL8A was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 10 μg with ab270979 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270979 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: SH-SY5Y whole cell lysate 10 μg

    Lane 2: ab270979 IP in SH-SY5Y whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab270979 in SH-SY5Y whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 76 seconds

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Immunoprecipitation - Anti-ARL8A antibody [EPR24376-2] (ab270979)

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL8A antibody [EPR24376-2] (ab270979), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL8A antibody [EPR24376-2] (ab270979)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling ARL8A with ab270979 at 1/50 (10.02 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab270979 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Cytoplasmic staining on mouse cerebrum.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL8A antibody [EPR24376-2] (ab270979), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL8A antibody [EPR24376-2] (ab270979)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling ARL8A with ab270979 at 1/50 (10.02 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab270979 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Cytoplasmic staining on rat cerebrum.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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