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Rabbit Recombinant Monoclonal ARL8A antibody. Suitable for ICC/IF, WB and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (AB281997), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] (AB281997), expandable thumbnail
  • Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (AB281997), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] (AB281997), expandable thumbnail
  • Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (AB281997), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PIHC-FrFlow Cyt (Intra)
Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Rat
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Plays a role in lysosome motility (By similarity). In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function (By similarity). May play a role in chromosome segregation (By similarity).

Additional Targets

ARL8B

Alternative names

ARL10B, GIE2, ARL8A, ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B, Novel small G protein indispensable for equal chromosome segregation 2

Recommended products

Rabbit Recombinant Monoclonal ARL8A antibody. Suitable for ICC/IF, WB and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24376-103
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ARL8A and ARL8B also known as ADP-ribosylation factor-like protein 8A and 8B are small GTPases with masses of approximately 21 kDa. They belong to the ARF-like GTPase family and they localize mainly on lysosomes and late endosomes. Both ARL8A and ARL8B widely express in tissues but show higher levels in certain types like immune cells. Their structural and functional similarities allow them to share roles in cellular functions related to lysosomal positioning and movement.

Biological function summary

ARL8A and ARL8B regulate the anterograde transport of lysosomes and are part of the lysosomal trafficking complex. They help in organizing lysosomal positioning a role important for normal lysosome function. This function includes interaction and formation of a complex with other proteins such as PLEKHM2 and SKIP which facilitate the attachment of lysosomes to microtubule tracks via kinesin motor proteins. This movement allows lysosomes to fulfill their roles in cellular distribution and degradation processes.

Pathways

ARL8A and ARL8B play roles in the lysosomal protein transport pathway and autophagy. They engage in lysosomal motility impacting autophagic flux a process essential for cellular homeostasis and stress response. ARL8B in particular interacts with the HOPS complex promoting fusion between autophagosomes and lysosomes therefore influencing autophagy. Among related proteins Rab7A is noteworthy as a regulator of late endosomal trafficking pathways that interface with processes involving ARL8 proteins.

Associated diseases and disorders

ARL8A and ARL8B are associated with neurodegenerative diseases and cancer. Dysregulation in lysosomal positioning can impair autophagy linking these proteins to neurodegenerative conditions like Parkinson's disease. In cancer altered lysosomal trafficking influences tumor cell invasion and metastasis. Both proteins interact with LAMP proteins which contribute to the lysosomal membrane integrity central to diseases involving lysosomal dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (ab281997), expandable thumbnail

    Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (ab281997)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 26 seconds.

    All lanes: Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (ab281997) at 1/1000 dilution

    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg

    Lane 2: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg

    Lane 3: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 4: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 5: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg

    Lane 6: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 40 µg

    Lane 7: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 40 µg

    Lane 8: C6 (rat glial tumor glial cell) whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Observed band size: 21-22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] (ab281997), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] (ab281997)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 cells labelling ARL8A+ARL8B with ab281997 at 1/50 (10.24 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

    Confocal image showing cytoplasmic staining in C6 cell line.

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (ab281997), expandable thumbnail

    Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (ab281997)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.
    Exposure time: 37 seconds.

    All lanes: Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (ab281997) at 1/1000 dilution

    Lane 1: Human placenta tissue lysate at 40 µg

    Lane 2: Mouse brain tissue lysate at 40 µg

    Lane 3: Rat brain tissue lysate at 40 µg

    Lane 4: Rat placenta tissue lysate at 40 µg

    Secondary

    Lane 1: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Lanes 2 - 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Observed band size: 21-22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] (ab281997), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] (ab281997)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a cells labelling ARL8A+ARL8B with ab281997 at 1/50 (10.24 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

    Confocal image showing cytoplasmic staining in Neuro-2a cell line.

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (ab281997), expandable thumbnail

    Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (ab281997)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (ab281997) at 1/1000 dilution

    Lane 1: His-tagged human ARL8A recombinant protein at 0.02 µg

    Lane 2: His-tagged human ARL8B recombinant protein at 0.02 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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