Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030)

This data was developed using ab281997, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 cells labelling ARL8A+ARL8B with ab281997 at 1/50 (10.24 μg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in C6 cell line.

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030)

This data was developed using ab281997, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a cells labelling ARL8A+ARL8B with ab281997 at 1/50 (10.24 μg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in Neuro-2a cell line.

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

• WB

Lab

Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030)

This data was developed using ab281997, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 26 seconds.

All lanes:

Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (<a href='/en-us/products/primary-antibodies/arl8a-arl8b-antibody-epr24376-103-ab281997'>ab281997</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg

Lane 3:

SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 4:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg

Lane 6:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 40 µg

Lane 7:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 40 µg

Lane 8:

C6 (rat glial tumor glial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 21-22 kDa

false

• WB

Lab

Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030)

This data was developed using ab281997, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 37 seconds.

All lanes:

Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (<a href='/en-us/products/primary-antibodies/arl8a-arl8b-antibody-epr24376-103-ab281997'>ab281997</a>) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 40 µg

Lane 2:

Mouse brain tissue lysate at 40 µg

Lane 3:

Rat brain tissue lysate at 40 µg

Lane 4:

Rat placenta tissue lysate at 40 µg

Secondary

Lane 1:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lanes 2 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 21-22 kDa

false

• WB

Lab

Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030)

This data was developed using ab281997, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (<a href='/en-us/products/primary-antibodies/arl8a-arl8b-antibody-epr24376-103-ab281997'>ab281997</a>) at 1/1000 dilution

Lane 1:

His-tagged human ARL8A recombinant protein at 0.02 µg

Lane 2:

His-tagged human ARL8B recombinant protein at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false