Rabbit Recombinant Monoclonal ARL8A antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030), expandable thumbnail](https://content.abcam.com/products/images/arl8a-arl8b-antibody-epr24376-103-bsa-and-azide-free-ab282030--western-blot-img165484.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030)")
![Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030), expandable thumbnail](https://content.abcam.com/products/images/arl8a-arl8b-antibody-epr24376-103-bsa-and-azide-free-ab282030--immunocytochemistry-immunofluorescence-img165882.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030)")
![Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030), expandable thumbnail](https://content.abcam.com/products/images/arl8a-arl8b-antibody-epr24376-103-bsa-and-azide-free-ab282030--western-blot-img169888.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030)")
![Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030), expandable thumbnail](https://content.abcam.com/products/images/arl8a-arl8b-antibody-epr24376-103-bsa-and-azide-free-ab282030--immunocytochemistry-immunofluorescence-img170380.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030)")
![Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030), expandable thumbnail](https://content.abcam.com/products/images/arl8a-arl8b-antibody-epr24376-103-bsa-and-azide-free-ab282030--western-blot-img170225.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (AB282030)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | IHC-Fr | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Target data
Function
Plays a role in lysosome motility (By similarity). In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function (By similarity). May play a role in chromosome segregation (By similarity).
Additional Targets
ARL8A
Alternative names
ARL10B, GIE2, ARL8A, ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B, Novel small G protein indispensable for equal chromosome segregation 2
Recommended products
Rabbit Recombinant Monoclonal ARL8A antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24376-103
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab282030 is the carrier-free version of Anti-ARL8A + ARL8B antibody [EPR24376-103] ab281997.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARL8A and ARL8B also known as ADP-ribosylation factor-like protein 8A and 8B are small GTPases with masses of approximately 21 kDa. They belong to the ARF-like GTPase family and they localize mainly on lysosomes and late endosomes. Both ARL8A and ARL8B widely express in tissues but show higher levels in certain types like immune cells. Their structural and functional similarities allow them to share roles in cellular functions related to lysosomal positioning and movement.
- Biological function summary
ARL8A and ARL8B regulate the anterograde transport of lysosomes and are part of the lysosomal trafficking complex. They help in organizing lysosomal positioning a role important for normal lysosome function. This function includes interaction and formation of a complex with other proteins such as PLEKHM2 and SKIP which facilitate the attachment of lysosomes to microtubule tracks via kinesin motor proteins. This movement allows lysosomes to fulfill their roles in cellular distribution and degradation processes.
- Pathways
ARL8A and ARL8B play roles in the lysosomal protein transport pathway and autophagy. They engage in lysosomal motility impacting autophagic flux a process essential for cellular homeostasis and stress response. ARL8B in particular interacts with the HOPS complex promoting fusion between autophagosomes and lysosomes therefore influencing autophagy. Among related proteins Rab7A is noteworthy as a regulator of late endosomal trafficking pathways that interface with processes involving ARL8 proteins.
- Associated diseases and disorders
ARL8A and ARL8B are associated with neurodegenerative diseases and cancer. Dysregulation in lysosomal positioning can impair autophagy linking these proteins to neurodegenerative conditions like Parkinson's disease. In cancer altered lysosomal trafficking influences tumor cell invasion and metastasis. Both proteins interact with LAMP proteins which contribute to the lysosomal membrane integrity central to diseases involving lysosomal dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030), expandable thumbnail](https://content.abcam.com/products/images/arl8a-arl8b-antibody-epr24376-103-bsa-and-azide-free-ab282030--western-blot-img165484.jpg "Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030)")
Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030)
This data was developed using Anti-ARL8A + ARL8B antibody [EPR24376-103] ab281997, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 26 seconds.
All lanes: Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (Anti-ARL8A + ARL8B antibody [EPR24376-103] ab281997) at 1/1000 dilution
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg
Lane 2: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg
Lane 3: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg
Lane 4: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg
Lane 5: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg
Lane 6: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 40 µg
Lane 7: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 40 µg
Lane 8: C6 (rat glial tumor glial cell) whole cell lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Observed band size: 21-22 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030), expandable thumbnail](https://content.abcam.com/products/images/arl8a-arl8b-antibody-epr24376-103-bsa-and-azide-free-ab282030--immunocytochemistry-immunofluorescence-img165882.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030)")
Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030)
This data was developed using Anti-ARL8A + ARL8B antibody [EPR24376-103] ab281997, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 cells labelling ARL8A+ARL8B with Anti-ARL8A + ARL8B antibody [EPR24376-103] ab281997 at 1/50 (10.24 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
Confocal image showing cytoplasmic staining in C6 cell line.
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030), expandable thumbnail](https://content.abcam.com/products/images/arl8a-arl8b-antibody-epr24376-103-bsa-and-azide-free-ab282030--western-blot-img169888.jpg "Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030)")
Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030)
This data was developed using Anti-ARL8A + ARL8B antibody [EPR24376-103] ab281997, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 37 seconds.
All lanes: Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (Anti-ARL8A + ARL8B antibody [EPR24376-103] ab281997) at 1/1000 dilution
Lane 1: Human placenta tissue lysate at 40 µg
Lane 2: Mouse brain tissue lysate at 40 µg
Lane 3: Rat brain tissue lysate at 40 µg
Lane 4: Rat placenta tissue lysate at 40 µg
SecondaryLane 1: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Lanes 2 - 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Observed band size: 21-22 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030), expandable thumbnail](https://content.abcam.com/products/images/arl8a-arl8b-antibody-epr24376-103-bsa-and-azide-free-ab282030--immunocytochemistry-immunofluorescence-img170380.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030)")
Immunocytochemistry/ Immunofluorescence - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030)
This data was developed using Anti-ARL8A + ARL8B antibody [EPR24376-103] ab281997, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a cells labelling ARL8A+ARL8B with Anti-ARL8A + ARL8B antibody [EPR24376-103] ab281997 at 1/50 (10.24 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
Confocal image showing cytoplasmic staining in Neuro-2a cell line.
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030), expandable thumbnail](https://content.abcam.com/products/images/arl8a-arl8b-antibody-epr24376-103-bsa-and-azide-free-ab282030--western-blot-img170225.jpg "Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030)")
Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free (ab282030)
This data was developed using Anti-ARL8A + ARL8B antibody [EPR24376-103] ab281997, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Western blot - Anti-ARL8A + ARL8B antibody [EPR24376-103] (Anti-ARL8A + ARL8B antibody [EPR24376-103] ab281997) at 1/1000 dilution
Lane 1: His-tagged human ARL8A recombinant protein at 0.02 µg
Lane 2: His-tagged human ARL8B recombinant protein at 0.02 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
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