Anti-ARMET/ARP antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ARMET/ARP antibody (AB67271)

Immunofluorescent analysis of 4% paraformaldehydefixed human brain cells labeling ARMET/ARP in Human Brain cells using ab67271 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARMET/ARP antibody (AB67271)

ab67271 at 2.5 μg/ml staining of ARMET/ARP in human brain tissue using IHC-P. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARMET/ARP antibody (AB67271)

ab67271 at 2.5 μg/ml staining of ARMET/ARP in human brain tissue using IHC-P. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• WB

Supplier Data

Western blot - Anti-ARMET/ARP antibody (AB67271)

Diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ARMET/ARP antibody (ab67271) at 1 µg/mL

Lane 1:

Wild-type HEK293T cell lysate at 10 µg

Lane 2:

ARMET/ARP knock-out HEK293T cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) at 1/10000 dilution

Predicted band size: 20 kDa

Observed band size: 17 kDa

false

• WB

Supplier Data

Western blot - Anti-ARMET/ARP antibody (AB67271)

Blocking conditions : 5% non-fat milk in TBS at 4 overnight.

Primary antibody : Room temperature for 1 hour

Diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ARMET/ARP antibody (ab67271) at 2 µg/mL

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 15 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) cell lysate at 15 µg

Lane 3:

A549 (human lung carcinoma cell line) cell lysate at 15 µg

Lane 4:

CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate at 15 µg

Lane 5:

Daudi (human Burkitt's lymphoma cell line) cell lysate at 15 µg

Lane 6:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

Lane 7:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg

Lane 8:

SK-N-SH (human neuroblastoma cell line) cell lysate at 15 µg

Lane 9:

THP-1 (human monocytic leukemia cell line) cell lysate at 15 µg

Lane 10:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 15 µg

Lane 11:

YB2/0 (rat B cell lymphoblast cell line) cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG (HRP) at 1/10000 dilution

Predicted band size: 20 kDa

Observed band size: 17 kDa

false