Rabbit Polyclonal ARMET/ARP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human MANF.
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- Acta pharmaceutica Sinica. B 13:4234-42522023MANF brakes TLR4 signaling by competitively binding S100A8 with S100A9 to regulate macrophage phenotypes in hepatic fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Hou,Dong Wang,Mingxia Zhao,Petek Ballar,Xinru Zhang,Qiong Mei,Wei Wang,Xiang Li,Qiang Sheng,Jun Liu,Chuansheng Wei,Yujun Shen,Yi Yang,Peng Wang,Juntang Shao,Sa Xu,Fuyan Wang,Yang Sun,Yuxian ShenPubMed 37799387
- Cell death & disease 13:1002022Mesencephalic astrocyte-derived neurotrophic factor reprograms macrophages to ameliorate acetaminophen-induced acute liver injury via p38 MAPK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Hou,Qi Liu,Yimin Gao,Liang Yong,Huiyuan Xie,Wenting Li,Yuping Zhou,Jun Liu,Lijie Feng,Long Xu,Yuxian Shen,Hua WangPubMed 35110525
- International journal of molecular sciences 23:2021New Proteins Contributing to Immune Cell Infiltration and Pannus Formation of Synovial Membrane from Arthritis Diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDominique de Seny,Dominique Baiwir,Elettra Bianchi,Gaël Cobraiville,Céline Deroyer,Christophe Poulet,Olivier Malaise,Geneviève Paulissen,Marie-Joëlle Kaiser,Jean-Philippe Hauzeur,Gabriel Mazzucchelli,Philippe Delvenne,Michel MalaisePubMed 35008858
- Frontiers in aging neuroscience 13:6393182021Increased MANF Expression in the Inferior Temporal Gyrus in Patients With Alzheimer Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue-Chun Liu,Xiu-Hong Qi,Hui Fang,Ke-Qing Zhou,Qing-Song Wang,Gui-Hai ChenPubMed 33994992
- Frontiers in cell and developmental biology 9:5799512021MANF Produced by MRL Mouse-Derived Mesenchymal Stem Cells Is Pro-regenerative and Protects From Osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGautier Tejedor,Patricia Luz-Crawford,Audrey Barthelaix,Karine Toupet,Sébastien Roudières,François Autelitano,Christian Jorgensen,Farida DjouadPubMed 33738280
- Journal of neuroinflammation 17:3092020Neuroprotective effects of verbascoside against Alzheimer's disease via the relief of endoplasmic reticulum stress in Aβ-exposed U251 cells and APP/PS1 mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunyue Wang et. al.PubMed 33070776
- Frontiers in molecular neuroscience 13:5600202020Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) Regulates Neurite Outgrowth Through the Activation of Akt/mTOR and Erk/mTOR Signaling Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Wen et. al.PubMed 33071755
- OncoTargets and therapy 13:9169-91842020Mesencephalic Astrocyte-Derived Neurotrophic Factor, a Prognostic Factor of Cholangiocarcinoma, Affects Sorafenib Sensitivity of Cholangiocarcinoma Cells by Deteriorating ER Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingyi He et. al.PubMed 32982305
- Evidence-based complementary and alternative medic 2020:18183072020Mechanisms of Cong Rong Shu Jing Compound Effects on Endoplasmic Reticulum Stress in a Rat Model of Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Lin et. al.PubMed 32508943
- World journal of gastroenterology 26:1029-10412020Mesencephalic astrocyte-derived neurotrophic factor ameliorates steatosis in HepG2 cells by regulating hepatic lipid metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiao He,Cong Wang,Xiao-Hong Long,Jia-Jia Peng,Dong-Fang Liu,Gang-Yi Yang,Michael D Jensen,Li-Li ZhangPubMed 32205994
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MANF. The exact immunogen used to generate this antibody is proprietary information. Database link P55145
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.125-2 µg/mL | Notes Blocking conditions: 5% non-fat milk in TBS at 4 overnight. |
Species Rat | Dilution info 0.125-2 µg/mL | Notes Blocking conditions: 5% non-fat milk in TBS at 4 overnight. |
Species Human | Dilution info 0.125-2 µg/mL | Notes Blocking conditions: 5% non-fat milk in TBS at 4 overnight. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.5 µg/mL | Notes Not validated in mouse and rat samples |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 20 µg/mL | Notes Not validated in mouse and rat samples |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain (PubMed:12794311). Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (By similarity). Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death (PubMed:18561914, PubMed:22637475, PubMed:29497057). Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions (PubMed:22637475). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed:22637475). Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells (PubMed:29497057). Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions (PubMed:29497057).
Alternative names
ARMET, ARP, MANF, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich protein, Protein ARMET
Recommended products
Rabbit Polyclonal ARMET/ARP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human MANF.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MANF. The exact immunogen used to generate this antibody is proprietary information. Database link P55145
- Purification technique
- Affinity purification Protein A
- Specificity
ab67271 does not crossreact with CDNF.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Please see Anti-ARMET/ARP antibody [EPR22538-206] ab253243 as alternative product for testing human samples in WB and ICC/IF.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ARMET also known as Arp is a 19 kDa protein known for its role in cellular stress responses. It is alternatively named MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor) in some studies. This protein is widely expressed including in the brain liver and pancreas reflecting its involvement across different cellular environments. ARMET/ARP interacts with the endoplasmic reticulum to address misfolded protein loads and support cell survival under stress.
- Biological function summary
ARMET plays a significant role in maintaining cellular homeostasis especially under stress conditions. It does not form a larger complex but functions as a standalone factor that aids in the proper folding of proteins within the endoplasmic reticulum. Additionally ARMET has protective effects on dopaminergic neurons which are critical for neuromolecular processes. This protein has connections to cellular survival pathways acting as a neurotrophic factor to support neuron maintenance and health.
- Pathways
ARMET is an important player in the unfolded protein response (UPR) and the neuroprotective pathways. In these pathways ARMET interacts with other proteins involved in protein folding and stress responses such as BiP/GRP78. These interactions help prevent apoptosis and promote cell survival by ensuring that cells can cope with endoplasmic reticulum stress. The ability of ARMET to enhance neuronal survival links it to pathways important for neuroprotection.
- Associated diseases and disorders
ARMET has been associated with neurodegenerative diseases such as Parkinson's disease and diabetes. In Parkinson's disease the survival-promoting effects of ARMET on dopaminergic neurons become vital potentially offering therapeutic targets. Connections between ARMET and diabetes surface through its regulation of beta-cell survival in the pancreas suggesting a role in maintaining insulin production and glucose homeostasis. In these diseases ARMET may interact with other proteins like proinsulin which holds significance for maintaining endocrine functions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-ARMET/ARP antibody (ab67271)
Blocking conditions: 5% non-fat milk in TBS at 4 overnight.
Primary antibody: Room temperature for 1 hour
Diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-ARMET/ARP antibody (ab67271) at 2 µg/mL
Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 15 µg
Lane 2: A431 (human epidermoid carcinoma cell line) cell lysate at 15 µg
Lane 3: A549 (human lung carcinoma cell line) cell lysate at 15 µg
Lane 4: CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate at 15 µg
Lane 5: Daudi (human Burkitt's lymphoma cell line) cell lysate at 15 µg
Lane 6: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg
Lane 7: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg
Lane 8: SK-N-SH (human neuroblastoma cell line) cell lysate at 15 µg
Lane 9: THP-1 (human monocytic leukemia cell line) cell lysate at 15 µg
Lane 10: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 15 µg
Lane 11: YB2/0 (rat B cell lymphoblast cell line) cell lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARMET/ARP antibody (ab67271)
ab67271 at 2.5 μg/ml staining of ARMET/ARP in human brain tissue using IHC-P. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-ARMET/ARP antibody (ab67271)
Diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-ARMET/ARP antibody (ab67271) at 1 µg/mL
Lane 1: Wild-type HEK293T cell lysate at 10 µg
Lane 2: ARMET/ARP knock-out HEK293T cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARMET/ARP antibody (ab67271)
ab67271 at 2.5 μg/ml staining of ARMET/ARP in human brain tissue using IHC-P. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-ARMET/ARP antibody (ab67271)
Immunofluorescent analysis of 4% paraformaldehydefixed human brain cells labeling ARMET/ARP in Human Brain cells using ab67271 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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