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AB253243

Anti-ARMET/ARP antibody [EPR22538-206]

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(1 Publication)

Rabbit Recombinant Monoclonal ARMET/ARP antibody. Suitable for I-ELISA, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ARMET, ARP, MANF, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich protein, Protein ARMET

6 Images
Flow Cytometry (Intracellular) - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling ARMET/ARP with ab253243 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ARMET/ARP with ab253243 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in HeLa cell line. The nuclear counterstain is DAPI (blue). Counterstained with ab12223 Anti-KDEL antibody - Endoplasmic reticulum Marker (Mouse monoclonal) ab150120 AlexaFluor®594 Goat anti-Mouse secondary.

Secondary antibody only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunoprecipitation - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243)
  • IP

Unknown

Immunoprecipitation - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243)

ARMET/ARP was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab253243 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab253243 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

Lane 1 : HeLa whole cell lysate 10 μg (Input).
Lane 2 : ab253243 IP in HeLa whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab253243 in HeLa whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

Predicted band size: 20 kDa

Observed band size: 18 kDa

false

Indirect ELISA - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243)
  • I-ELISA

Lab

Indirect ELISA - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243)

ELISA analysis of Human ARMET protein at 1000 ng/mL with ab253243 at 1000-0 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243)
  • WB

Unknown

Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243)

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 22637475).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 20 kDa

Observed band size: 18 kDa

false

Exposure time: 59s

Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243)
  • WB

Unknown

Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243)

No cross reactivity was found for CDNF.

Blocking/Dilution : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243) at 1/1000 dilution

Lane 1:

His-tagged human ARMET/ARP recombinant protein 20 ng

Lane 2:

His-tagged human CDNF recombinant protein 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 20 kDa

Observed band size: 18 kDa

false

Exposure time: 10s

  • Carrier free

    Anti-ARMET/ARP antibody [EPR22538-206] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22538-206

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, IP, I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/600", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARMET also known as Arp is a 19 kDa protein known for its role in cellular stress responses. It is alternatively named MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor) in some studies. This protein is widely expressed including in the brain liver and pancreas reflecting its involvement across different cellular environments. ARMET/ARP interacts with the endoplasmic reticulum to address misfolded protein loads and support cell survival under stress.
Biological function summary

ARMET plays a significant role in maintaining cellular homeostasis especially under stress conditions. It does not form a larger complex but functions as a standalone factor that aids in the proper folding of proteins within the endoplasmic reticulum. Additionally ARMET has protective effects on dopaminergic neurons which are critical for neuromolecular processes. This protein has connections to cellular survival pathways acting as a neurotrophic factor to support neuron maintenance and health.

Pathways

ARMET is an important player in the unfolded protein response (UPR) and the neuroprotective pathways. In these pathways ARMET interacts with other proteins involved in protein folding and stress responses such as BiP/GRP78. These interactions help prevent apoptosis and promote cell survival by ensuring that cells can cope with endoplasmic reticulum stress. The ability of ARMET to enhance neuronal survival links it to pathways important for neuroprotection.

ARMET has been associated with neurodegenerative diseases such as Parkinson's disease and diabetes. In Parkinson's disease the survival-promoting effects of ARMET on dopaminergic neurons become vital potentially offering therapeutic targets. Connections between ARMET and diabetes surface through its regulation of beta-cell survival in the pancreas suggesting a role in maintaining insulin production and glucose homeostasis. In these diseases ARMET may interact with other proteins like proinsulin which holds significance for maintaining endocrine functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain (PubMed : 12794311). Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (By similarity). Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death (PubMed : 18561914, PubMed : 22637475, PubMed : 29497057). Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions (PubMed : 22637475). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed : 22637475). Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells (PubMed : 29497057). Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions (PubMed : 29497057).
See full target information MANF
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36362214

2022

Characterization of BCMA Expression in Circulating Rare Single Cells of Patients with Plasma Cell Neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Libere J Ndacayisaba,Kate E Rappard,Stephanie N Shishido,Sonia M Setayesh,Guilin Tang,Pei Lin,Nicholas Matsumoto,Ching-Ju Hsu,Rafael Nevarez,Carmen Ruiz Velasco,Amin Naghdloo,Eric Yang,Kevin Kelly,James Hicks,Jeremy Mason,Robert Z Orlowski,Elisabet E Manasanch,Peter Kuhn
View all publications
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