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Rabbit Recombinant Monoclonal ARMET/ARP antibody. Suitable for I-ELISA, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243), expandable thumbnail
  • Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243), expandable thumbnail
  • Immunoprecipitation - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243), expandable thumbnail
  • Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (AB253243), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
I-ELISAIPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/600
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain (PubMed:12794311). Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (By similarity). Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death (PubMed:18561914, PubMed:22637475, PubMed:29497057). Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions (PubMed:22637475). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed:22637475). Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells (PubMed:29497057). Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions (PubMed:29497057).

Alternative names

ARMET, ARP, MANF, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich protein, Protein ARMET

Recommended products

Rabbit Recombinant Monoclonal ARMET/ARP antibody. Suitable for I-ELISA, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22538-206
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ARMET also known as Arp is a 19 kDa protein known for its role in cellular stress responses. It is alternatively named MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor) in some studies. This protein is widely expressed including in the brain liver and pancreas reflecting its involvement across different cellular environments. ARMET/ARP interacts with the endoplasmic reticulum to address misfolded protein loads and support cell survival under stress.

Biological function summary

ARMET plays a significant role in maintaining cellular homeostasis especially under stress conditions. It does not form a larger complex but functions as a standalone factor that aids in the proper folding of proteins within the endoplasmic reticulum. Additionally ARMET has protective effects on dopaminergic neurons which are critical for neuromolecular processes. This protein has connections to cellular survival pathways acting as a neurotrophic factor to support neuron maintenance and health.

Pathways

ARMET is an important player in the unfolded protein response (UPR) and the neuroprotective pathways. In these pathways ARMET interacts with other proteins involved in protein folding and stress responses such as BiP/GRP78. These interactions help prevent apoptosis and promote cell survival by ensuring that cells can cope with endoplasmic reticulum stress. The ability of ARMET to enhance neuronal survival links it to pathways important for neuroprotection.

Associated diseases and disorders

ARMET has been associated with neurodegenerative diseases such as Parkinson's disease and diabetes. In Parkinson's disease the survival-promoting effects of ARMET on dopaminergic neurons become vital potentially offering therapeutic targets. Connections between ARMET and diabetes surface through its regulation of beta-cell survival in the pancreas suggesting a role in maintaining insulin production and glucose homeostasis. In these diseases ARMET may interact with other proteins like proinsulin which holds significance for maintaining endocrine functions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ARMET/ARP with ab253243 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in HeLa cell line. The nuclear counterstain is DAPI (blue). Counterstained with ab12223 Anti-KDEL antibody - Endoplasmic reticulum Marker (Mouse monoclonal) Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling ARMET/ARP with ab253243 at 1/600 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243), expandable thumbnail

    Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 22637475).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 20 kDa

    Observed band size: 18 kDa

    Exposure time: 59s

  • Immunoprecipitation - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243), expandable thumbnail

    Immunoprecipitation - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

    ARMET/ARP was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab253243 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab253243 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).
    Lane 2: ab253243 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab253243 in HeLa whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 15 seconds.

    All lanes: Immunoprecipitation - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

    Predicted band size: 20 kDa

    Observed band size: 18 kDa

  • Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243), expandable thumbnail

    Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

    No cross reactivity was found for CDNF.

    Blocking/Dilution: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243) at 1/1000 dilution

    Lane 1: His-tagged human ARMET/ARP recombinant protein 20 ng

    Lane 2: His-tagged human CDNF recombinant protein 20 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 20 kDa

    Observed band size: 18 kDa

    Exposure time: 10s

  • Indirect ELISA - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243), expandable thumbnail

    Indirect ELISA - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

    ELISA analysis of Human ARMET protein at 1000 ng/mL with ab253243 at 1000-0 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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