Anti-ARMET/ARP antibody [EPR29115-74]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ARMET/ARP with ab316935 at 1/2000 (0.253 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : no staining on human skeletal muscle. The section was incubated with ab316935 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human cerebrum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/500 dilution (C), and ARMET/ARP with ab316935 at a 1/1000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on human cerebrum.
Panel B : anti-Noradrenaline transporter staining nerves in human cerebrum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in human cerebrum.
Panel D : anti-ARMET/ARP staining neurons in human cerebrum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ARMET/ARP with ab316935 at 1/2000 (0.253 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on neurons of human cerebrum. The section was incubated with ab316935 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Immunohistochemical analysis of paraffin-embedded Rat P5 cerebrum tissue labeling ARMET/ARP with ab316935 at 1/10000 (0.051 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on neurons of rat P5 cerebrum. The section was incubated with ab316935 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labellingARMET/ARP with ab316935 at 1/50 (10.1 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution.

Confocal image showing cytoplasmic staining in NIH/3T3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution. The Nuclear counterstain was DAPI.

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling ARMET/ARP with ab316935 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebellum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on mouse cerebellum.
Panel B : anti-Noradrenaline transporter staining nerves in mouse cerebellum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in mouse cerebellum.
Panel D : anti-ARMET/ARP staining neurons in mouse cerebellum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling ARMET/ARP with ab316935 at 1/10000 (0.051 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : no staining on mouse skeletal muscle. The section was incubated with ab316935 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Immunohistochemical analysis of paraffin-embedded Mouse P5 cerebrum tissue labeling ARMET/ARP with ab316935 at 1/10000 (0.051 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on neurons of mouse cerebrum (PMID : 18718866). The section was incubated with ab316935 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat spinal cord tissue section labelling GPCR GPR17 with ab314307 at 1/2000 dilution (B), TAC1 with ab307138 at 1/500 dilution (C), and ARMET/ARP with ab316935 at 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-GPCR GPR17 (magenta; Opal™ 690), anti-TAC1 (green; Opal™ 520) and anti-ARMET/ARP (yellow; Opal™ 570) on rat spinal cord.
Panel B : anti-GPCR GPR17 staining oligodendrocytes in rat spinal cord.
Panel C : anti-TAC1 staining posterior horns (sensory) in rat spinal cord.
Panel D : anti-ARMET/ARP staining neurons in rat spinal cord.

The section was incubated in three rounds of staining : in the order of ab314307, ab307138 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue section labelling GPCR GPR17 with ab314307 at 1/2000 dilution (B), TAC1 with ab307138 at 1/500 dilution (C), and ARMET/ARP with ab316935 at 1/20000 (D). Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-GPCR GPR17 (magenta; Opal™ 690), anti-TAC1 (green; Opal™ 520) and anti-ARMET/ARP (yellow; Opal™ 570) on mouse spinal cord.
Panel B : anti-GPCR GPR17 staining oligodendrocytes in mouse spinal cord.
Panel C : anti-TAC1 staining posterior horns (sensory) in mouse spinal cord.
Panel D : anti-ARMET/ARP staining neurons in mouse spinal cord.

The section was incubated in three rounds of staining : in the order of ab314307, ab307138 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebrum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on mouse cerebrum.
Panel B : anti-Noradrenaline transporter staining nerves in mouse cerebrum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in mouse cerebrum.
Panel D : anti-ARMET/ARP staining neurons in mouse cerebrum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebellum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on rat cerebellum.
Panel B : anti-Noradrenaline transporter staining nerves in rat cerebellum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in rat cerebellum.
Panel D : anti-ARMET/ARP staining neurons in rat cerebellum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebrum tissue section labelling Noradrenaline transporter with ab254361 at a 1/100 dilution (B), GPCR GPR17 with ab314307 at a 1/2000 dilution (C), and ARMET/ARP with ab316935 at a 1/20000 dilution (D). Nuclear DNA was labeled with DAPI (shown in blue).

Panel A : merged staining of anti-Noradrenaline transporter (magenta; Opal™ 690), anti-GPCR GPR17 (green; Opal™ 520) and anti-ARMET/ARP (gray; Opal™ 570) on rat cerebrum.
Panel B : anti-Noradrenaline transporter staining nerves in rat cerebrum.
Panel C : anti-GPCR GPR17 staining oligodendrocytes in rat cerebrum.
Panel D : anti-ARMET/ARP staining neurons in rat cerebrum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The section was incubated in three rounds of staining : in the order of ab254361, ab314307 and ab316935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IP

Supplier Data

Immunoprecipitation - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

ARMET/ARP was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab316935 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316935 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : ab316935 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316935 in NIH/3T3 whole cell lysate

All lanes:

Immunoprecipitation - Anti-ARMET/ARP antibody [EPR29115-74] (ab316935) at 1/30 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with 5% NFDM/TBST

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Low expression : skeletal muscle (PMID : 31781038).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ARMET/ARP antibody [EPR29115-74] (ab316935) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 20 µg with 5% NFDM/TBST

Lane 2:

Human skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 18 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Exposure time : Lanes 1-3 : 180 seconds; Lanes 4-6 : 26 seconds.

All lanes:

Western blot - Anti-ARMET/ARP antibody [EPR29115-74] (ab316935) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 3:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 5:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 6:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa

false

• WB

Supplier Data

Western blot - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

Low expression : brain, cerebral cortex, heart, skeletal muscle (PMID : 24587361; PMID : 31781038).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ARMET/ARP antibody [EPR29115-74] (ab316935) at 1/1000 dilution

Lane 1:

Mouse P5 brain tissue lysate at 20 µg with 5% NFDM/TBST

Lane 2:

Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST

Lane 3:

Mouse cerebral cortex tissue lysate at 20 µg with 5% NFDM/TBST

Lane 4:

Mouse heart tissue lysate at 20 µg with 5% NFDM/TBST

Lane 5:

Mouse skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

Lane 6:

Rat P5 brain tissue lysate at 20 µg with 5% NFDM/TBST

Lane 7:

Rat brain tissue lysate at 20 µg with 5% NFDM/TBST

Lane 8:

Rat heart tissue lysate at 20 µg with 5% NFDM/TBST

Lane 9:

Rat skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa,36 kDa

false

Exposure time: 102s

• WB

Supplier Data

Western blot - Anti-ARMET/ARP antibody [EPR29115-74] (AB316935)

This antibody does not cross-react with mouse CDNF.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-ARMET/ARP antibody [EPR29115-74] (ab316935) at 1/1000 dilution

Lane 1:

His-tagged mouse MANF recombinant protein at 10 ng with 5% NFDM/TBST

Lane 2:

His-tagged mouse CDNF recombinant protein at 10 ng with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa

false

Exposure time: 26s