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AB327170

Anti-ARNT Antibody

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Rabbit Polyclonal HIF1 beta antibody. Suitable for IHC-P, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARNT.

View Alternative Names

BHLHE2, ARNT, Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, Hypoxia-inducible factor 1-beta, bHLHe2, HIF-1-beta, HIF1-beta

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARNT Antibody (AB327170)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARNT Antibody (AB327170)

Immunohistochemical staining of human cervix, uterine using ab327170 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in squamous epithelial cells.

Immunocytochemistry/ Immunofluorescence - Anti-ARNT Antibody (AB327170)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ARNT Antibody (AB327170)

Immunofluorescent staining of human cell line A-431 using ab327170 at 0.25–2 µg/ml, showing localization to nucleus & nuclear bodies.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARNT Antibody (AB327170)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARNT Antibody (AB327170)

Immunohistochemical staining of human placenta using ab327170 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in trophoblastic cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARNT Antibody (AB327170)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARNT Antibody (AB327170)

Immunohistochemical staining of human testis using ab327170 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in Leydig cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARNT Antibody (AB327170)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARNT Antibody (AB327170)

Immunohistochemical staining of human colon using ab327170 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in glandular cells.

ChIP - Anti-ARNT Antibody (AB327170)
  • ChIP

Supplier Data

ChIP - Anti-ARNT Antibody (AB327170)

ChIP-Exo-Seq composite graph for ab327170 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, ChIP

applications

Immunogen

Recombinant Fragment Protein within Human ARNT.

P27540

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for activity of the AHR. Upon ligand binding, AHR translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE). Not required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding (PubMed : 34521881). The complex initiates transcription of genes involved in the regulation of a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (Probable). The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT : AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed : 28396409).
See full target information ARNT
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Product promise

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