Anti-Aromatase antibody [1H1]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Aromatase antibody [1H1] (AB314002)

Immunofluorescence staining of HepG2 (Human liver hepatocellular carcinoma cell line) cells with ab314002 at 1 : 76, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aromatase antibody [1H1] (AB314002)

Immunohistochemical analysis of ab314002 diluted at 1 : 230 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• WB

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Western blot - Anti-Aromatase antibody [1H1] (AB314002)

All lanes:

Western blot - Anti-Aromatase antibody [1H1] (ab314002) at 2.3 µg/mL

Lane 1:

HepG2 whole cell lysate

Lane 2:

Jurkat whole cell lysate

Lane 3:

HEK293 whole cell lysate

Lane 4:

SH-SY5Y whole cell lysate

Lane 5:

U87 whole cell lysate

Lane 6:

K562 whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Observed band size: 58 kDa

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