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AB215443

Anti-Aromatase antibody

4

(1 Review)

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(1 Publication)

Rabbit Polyclonal Aromatase antibody. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CYP19A1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ARO1, CYAR, CYP19, CYP19A1, Aromatase, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aromatase antibody (AB215443)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aromatase antibody (AB215443)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling Aromatase with ab215443 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Flow Cytometry (Intracellular) - Anti-Aromatase antibody (AB215443)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Aromatase antibody (AB215443)

Intracellular flow cytometric analysis of RSC96 cells labeling Aromatase with ab215443 at 1/20 dilution for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aromatase antibody (AB215443)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aromatase antibody (AB215443)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling Aromatase with ab215443 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human CYP19A1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P11511

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aromatase also known as CYP19A is an enzyme responsible for the conversion of androgens into estrogens. It is a member of the cytochrome P450 superfamily and has a molecular mass of approximately 58 kDa. Aromatase is mainly expressed in the gonads brain adipose tissue and placenta. The activity of this enzyme is important for estrogen biosynthesis which is especially important in postmenopausal women where adipose tissue becomes an important estrogen source.
Biological function summary

The enzyme catalyzes the aromatization of androgens aiding in the production of estrogens like estradiol. This process is essential for maintaining estrogen levels in the human body. Aromatase operates as a monomer and does not form part of any larger complex. The balance of androgens and estrogens maintained by aromatase activity plays a significant role in reproductive functions and other physiological processes.

Pathways

Aromatase enzyme activity plays a pivotal role in the steroidogenesis pathway which involves the biosynthesis of steroid hormones. It is also a part of the estrogen signaling pathway interacting with other proteins like estrogen receptors. These pathways help regulate gene expression and are important in processes such as cell proliferation and differentiation linking aromatase function to broader metabolic and developmental effects.

Aromatase has a significant role in conditions such as hormone receptor-positive breast cancer and endometriosis. In breast cancer increased aromatase activity elevates estrogen levels promoting cancer cell growth. Anti-aromatase drugs like aromatase inhibitors target this enzyme to lower estrogen production. Additionally defects in aromatase activity can lead to disorders like aromatase deficiency resulting in an imbalance in sex hormone levels. Understanding its function aids in the development of therapies targeting proteins like the estrogen receptor which interacts with products of aromatase activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively (PubMed : 27702664, PubMed : 2848247). Catalyzes three successive oxidations of C19 androgens : two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid (PubMed : 20385561). Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen (PubMed : 22773874). Also displays 2-hydroxylase activity toward estrone (PubMed : 22773874). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed : 20385561, PubMed : 22773874).
See full target information CYP19A1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

General and comparative endocrinology 268:40-49 PubMed30055147

2018

Seasonal ovarian development in relation to the gonadotropins, steroids, aromatase and steroidogenic factor 1 (SF-1) in the banded gourami, Trichogaster fasciata.

Applications

Unspecified application

Species

Unspecified reactive species

Rajkumar Guchhait,Ankit Chatterjee,Dilip Mukherjee,Kousik Pramanick
View all publications
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