AB35604

Anti-Aromatase antibody

Name: Anti-Aromatase antibody (ab35604) | Abcam
Brand: Abcam Limited
SKU: AB35604
Price: 515 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(17 Publications)

Rabbit Polyclonal Aromatase antibody. Suitable for WB and reacts with Human samples. Cited in 17 publications.

View Alternative Names

ARO1, CYAR, CYP19, CYP19A1, Aromatase, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase

1 Images

Lab

Western blot - Anti-Aromatase antibody (AB35604)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab35604 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Aromatase antibody (ab35604) at 1 µg/mL

All lanes:

Human placenta tissue lysate - total protein (<a href='/en-us/products/unavailable/human-placenta-tissue-lysate-total-protein-ab29745'>ab29745</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 58 kDa

Observed band size: 55 kDa

true

Exposure time: 150s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aromatase also known as CYP19A is an enzyme responsible for the conversion of androgens into estrogens. It is a member of the cytochrome P450 superfamily and has a molecular mass of approximately 58 kDa. Aromatase is mainly expressed in the gonads brain adipose tissue and placenta. The activity of this enzyme is important for estrogen biosynthesis which is especially important in postmenopausal women where adipose tissue becomes an important estrogen source.

Biological function summary

The enzyme catalyzes the aromatization of androgens aiding in the production of estrogens like estradiol. This process is essential for maintaining estrogen levels in the human body. Aromatase operates as a monomer and does not form part of any larger complex. The balance of androgens and estrogens maintained by aromatase activity plays a significant role in reproductive functions and other physiological processes.

Pathways

Aromatase enzyme activity plays a pivotal role in the steroidogenesis pathway which involves the biosynthesis of steroid hormones. It is also a part of the estrogen signaling pathway interacting with other proteins like estrogen receptors. These pathways help regulate gene expression and are important in processes such as cell proliferation and differentiation linking aromatase function to broader metabolic and developmental effects.

Aromatase has a significant role in conditions such as hormone receptor-positive breast cancer and endometriosis. In breast cancer increased aromatase activity elevates estrogen levels promoting cancer cell growth. Anti-aromatase drugs like aromatase inhibitors target this enzyme to lower estrogen production. Additionally defects in aromatase activity can lead to disorders like aromatase deficiency resulting in an imbalance in sex hormone levels. Understanding its function aids in the development of therapies targeting proteins like the estrogen receptor which interacts with products of aromatase activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively (PubMed : 27702664, PubMed : 2848247). Catalyzes three successive oxidations of C19 androgens : two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid (PubMed : 20385561). Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen (PubMed : 22773874). Also displays 2-hydroxylase activity toward estrone (PubMed : 22773874). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed : 20385561, PubMed : 22773874).

See full target information CYP19A1

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 19:1502764 PubMed40035063

2025

Brain aromatase and its relationship with parental experience and behavior in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Duarte-Guterman,Dimitri A Skandalis,Ariane Merkl,Diana B Geissler,Günter Ehret

Veterinary sciences 9: PubMed36006309

2022

Hormonal Homologies between Canine Mammary Cancer and Human Breast Cancer in a Series of Cases.

Applications

Unspecified application

Species

Unspecified reactive species

Paloma Jimena de Andrés,Sara Cáceres,Juan Carlos Illera,Belén Crespo,Gema Silván,Felisbina Luisa Queiroga,Maria José Illera,Maria Dolores Pérez-Alenza,Laura Peña

Frontiers in endocrinology 12:666195 PubMed34531822

2021

Extracellular Vesicles Inhibit Proliferation and Invasion of Ovarian Endometrial Stromal Cells and Their Expression of SF-1, ERβ, and Aromatase.

Applications

Unspecified application

Species

Unspecified reactive species

XiaoQing Wang,PeiLi Wu,Xin Li,Cheng Zeng,JingWen Zhu,YingFang Zhou,Ye Lu,Qing Xue

Molecular and cellular biology 41:e0035721 PubMed34460330

2021

Regulation of Estradiol Synthesis by Aromatase Interacting Partner in Breast (AIPB).

Applications

Unspecified application

Species

Unspecified reactive species

Himangshu S Bose,Randy M Whittal,Curtis E Lanier,Brendan Marshall,Maheshinie Rajapaksha,Brian W Wheeler,Nicholas D Carbo,Elin M Hahn,Elizabeth W Perry,Neal M Hall,Mikhail M Melomed,Edward L Perkins,William E Burak

Oncology letters 16:4577-4585 PubMed30214592

2018

Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes invasion in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jieqi Ke,Zhen Shen,Min Li,Cheng Peng,Ping Xu,Meimei Wang,Yi Zhu,Xuefen Zhang,Dabao Wu

Journal of inorganic biochemistry 184:79-87 PubMed29684698

2018

Biophysical characterization of Aptenodytes forsteri cytochrome P450 aromatase.

Applications

Species

Unspecified reactive species

Francisco Zarate-Perez,Jesús B Velázquez-Fernández,Gareth K Jennings,Lisa S Shock,Charles E Lyons,John C Hackett

Scientific reports 7:17289 PubMed29229929

2017

Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer.

Applications

IHC-P

Species

Unspecified reactive species

Sreenivas Adurthi,Mahesh M Kumar,H S Vinodkumar,Geetashree Mukherjee,H Krishnamurthy,K Kshitish Acharya,U D Bafna,Devi K Uma,B Abhishekh,Sudhir Krishna,A Parchure,Murali Alka,R S Jayshree

Fertility and sterility 106:673-80 PubMed27320036

2016

Aromatase inhibitor regulates let-7 expression and let-7f-induced cell migration in endometrial cells from women with endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

SiHyun Cho,Levent Mutlu,Yuping Zhou,Hugh S Taylor

Veterinary pathology 52:1045-51 PubMed25883120

2015

Effects of Obesity and Obesity-Related Molecules on Canine Mammary Gland Tumors.

Applications

IHC-P

Species

Dog

H-Y Lim,K-S Im,N-H Kim,H-W Kim,J-I Shin,J-Y Yhee,J-H Sur

Stem cells translational medicine 4:261-8 PubMed25666845

2015

Functional maintenance of differentiated embryoid bodies in microfluidic systems: a platform for personalized medicine.

Applications

ICC/IF

Species

Mouse

Sinan Guven,Jennifer S Lindsey,Ishwari Poudel,Sireesha Chinthala,Michael D Nickerson,Behzad Gerami-Naini,Umut A Gurkan,Raymond M Anchan,Utkan Demirci

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aromatase antibody

Western blot - Anti-Aromatase antibody (AB35604)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

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