AB191093

Anti-Aromatase antibody - C-terminal

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(4 Publications)

Rabbit Polyclonal Aromatase antibody. C-terminal. Suitable for IHC-P, WB and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Rat Cyp19a1 aa 450-500.

View Alternative Names

Arom, Cyp19, Cyp19a1, Aromatase, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aromatase antibody - C-terminal (AB191093)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aromatase antibody - C-terminal (AB191093)

Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling Aromatase with ab191093 at 1 μg/ml.

Supplier Data

Western blot - Anti-Aromatase antibody - C-terminal (AB191093)

The detection limit for ab191093 is approximately 5 ng/lane under reducing conditions.

All lanes:

Western blot - Anti-Aromatase antibody - C-terminal (ab191093) at 0.5 µg/mL

Lane 1:

Rat brain tissue lysates

Lane 2:

Rat thymus tissue lysates

Lane 3:

Rat kidney tissue lysates

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Rat Cyp19a1 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

P22443

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aromatase also known as CYP19A is an enzyme responsible for the conversion of androgens into estrogens. It is a member of the cytochrome P450 superfamily and has a molecular mass of approximately 58 kDa. Aromatase is mainly expressed in the gonads brain adipose tissue and placenta. The activity of this enzyme is important for estrogen biosynthesis which is especially important in postmenopausal women where adipose tissue becomes an important estrogen source.

Biological function summary

The enzyme catalyzes the aromatization of androgens aiding in the production of estrogens like estradiol. This process is essential for maintaining estrogen levels in the human body. Aromatase operates as a monomer and does not form part of any larger complex. The balance of androgens and estrogens maintained by aromatase activity plays a significant role in reproductive functions and other physiological processes.

Pathways

Aromatase enzyme activity plays a pivotal role in the steroidogenesis pathway which involves the biosynthesis of steroid hormones. It is also a part of the estrogen signaling pathway interacting with other proteins like estrogen receptors. These pathways help regulate gene expression and are important in processes such as cell proliferation and differentiation linking aromatase function to broader metabolic and developmental effects.

Aromatase has a significant role in conditions such as hormone receptor-positive breast cancer and endometriosis. In breast cancer increased aromatase activity elevates estrogen levels promoting cancer cell growth. Anti-aromatase drugs like aromatase inhibitors target this enzyme to lower estrogen production. Additionally defects in aromatase activity can lead to disorders like aromatase deficiency resulting in an imbalance in sex hormone levels. Understanding its function aids in the development of therapies targeting proteins like the estrogen receptor which interacts with products of aromatase activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the formation of aromatic C18 estrogens from C19 androgens.

See full target information Cyp19a1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 14: PubMed36297046

2022

Kelulut Honey Improves Folliculogenesis, Steroidogenic, and Aromatase Enzyme Profiles and Ovarian Histomorphology in Letrozole-Induced Polycystic Ovary Syndrome Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Datu Agasi Mohd Kamal,Siti Fatimah Ibrahim,Azizah Ugusman,Siti Sarah Mohamad Zaid,Mohd Helmy Mokhtar

Biomolecules 12: PubMed35740892

2022

Gut Steroids and Microbiota: Effect of Gonadectomy and Sex.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Diviccaro,Jamie A FitzGerald,Lucia Cioffi,Eva Falvo,Fiona Crispie,Paul D Cotter,Siobhain M O'Mahony,Silvia Giatti,Donatella Caruso,Roberto Cosimo Melcangi

Gynecological endocrinology : the official journal :1-8 PubMed33150797

2020

Preliminary study of Yulin mixture affecting the miR-320/SF-1/Cyp19a1 on mouse polycystic ovary syndrome model.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxuan Zhang,Lingling Xu

eNeuro 5: PubMed30406178

2018

Distal Dendritic Enrichment of HCN1 Channels in Hippocampal CA1 Is Promoted by Estrogen, but Does Not Require Reelin.

Applications

Unspecified application

Species

Unspecified reactive species

Maurice Meseke,Florian Neumüller,Bianka Brunne,Xiaoyu Li,Max Anstötz,Theresa Pohlkamp,Meike M Rogalla,Joachim Herz,Gabriele M Rune,Roland A Bender

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aromatase antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aromatase antibody - C-terminal (AB191093)

Western blot - Anti-Aromatase antibody - C-terminal (AB191093)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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