Rabbit Polyclonal ARP1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ACTR1A.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ACTR1A. The exact immunogen used to generate this antibody is proprietary information. Database link P61163
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Dog, Xenopus laevis, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Dog, Xenopus laevis, Rhesus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules.
Alternative names
CTRN1, ACTR1A, Alpha-centractin, Centractin, ARP1, Actin-RPV, Centrosome-associated actin homolog
Recommended products
Rabbit Polyclonal ARP1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ACTR1A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ACTR1A. The exact immunogen used to generate this antibody is proprietary information. Database link P61163
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein ARP1 also known as Actin-Related Protein 1 functions as an important component of the cytoskeleton organization in eukaryotic cells. It is a member of the actin family and shares structural similarities with conventional actin. ARP1 has a mass of approximately 42 kDa. It localizes mostly in the cytoplasm but is also found in the nucleus to some extent. The protein is expressed in a variety of tissues with notable expression in neural tissues.
- Biological function summary
ARP1 plays a significant role in cellular processes beyond maintaining structural integrity. It forms part of the dynactin complex which is important for dynein-driven cargo movement along microtubules. This complex includes several other proteins such as p150Glued and dynamitin. By participating in these assemblies ARP1 facilitates vesicular trafficking and is involved in processes like endocytosis and mitosis.
- Pathways
ARP1 contributes to vital cellular networks that drive intracellular transport and mitotic spindle orientation. One important pathway where ARP1 acts is the dynein-mediated transport pathway. Within this network ARP1 partners with the protein dynein to facilitate the movement of vesicles and organelles. Another critical pathway is related to mitotic spindle formation where ARP1 influences the positioning and dynamics of the spindle apparatus ensuring accurate chromosome segregation.
- Associated diseases and disorders
ARP1 possesses connections to neurodegenerative diseases and cancer. Alterations in ARP1 function can lead to impaired axonal transport contributing to conditions like Alzheimer's disease. In the context of cancer ARP1 mutations or dysregulation can disrupt normal cellular division and polarity potentially promoting oncogenesis. The association of ARP1 with p150Glued further links it to neurological diseases as mutations in these proteins can exacerbate cellular dysfunction in neurons.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARP1 antibody (ab227425)
Paraffin-embedded human lung cancer tissue stained for ARP1 using ab227425 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-ARP1 antibody (ab227425)
10% SDS-PAGE
All lanes: Western blot - Anti-ARP1 antibody (ab227425) at 1/2000 dilution
All lanes: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Western blot - Anti-ARP1 antibody (ab227425)
10% SDS-PAGE
All lanes: Western blot - Anti-ARP1 antibody (ab227425) at 1/1000 dilution
All lanes: Mouse liver tissue lysate at 50 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Downloads
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