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AB227425

Anti-ARP1 antibody

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(1 Publication)

Rabbit Polyclonal ARP1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ACTR1A.

View Alternative Names

CTRN1, ACTR1A, Alpha-centractin, Centractin, ARP1, Actin-RPV, Centrosome-associated actin homolog

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARP1 antibody (AB227425)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARP1 antibody (AB227425)

Paraffin-embedded human lung cancer tissue stained for ARP1 using ab227425 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-ARP1 antibody (AB227425)
  • WB

Supplier Data

Western blot - Anti-ARP1 antibody (AB227425)

10% SDS-PAGE

All lanes:

Western blot - Anti-ARP1 antibody (ab227425) at 1/2000 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Predicted band size: 43 kDa

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Western blot - Anti-ARP1 antibody (AB227425)
  • WB

Supplier Data

Western blot - Anti-ARP1 antibody (AB227425)

10% SDS-PAGE

All lanes:

Western blot - Anti-ARP1 antibody (ab227425) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate at 50 µg

Predicted band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ACTR1A. The exact immunogen used to generate this antibody is proprietary information.

P61163

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ARP1 also known as Actin-Related Protein 1 functions as an important component of the cytoskeleton organization in eukaryotic cells. It is a member of the actin family and shares structural similarities with conventional actin. ARP1 has a mass of approximately 42 kDa. It localizes mostly in the cytoplasm but is also found in the nucleus to some extent. The protein is expressed in a variety of tissues with notable expression in neural tissues.
Biological function summary

ARP1 plays a significant role in cellular processes beyond maintaining structural integrity. It forms part of the dynactin complex which is important for dynein-driven cargo movement along microtubules. This complex includes several other proteins such as p150Glued and dynamitin. By participating in these assemblies ARP1 facilitates vesicular trafficking and is involved in processes like endocytosis and mitosis.

Pathways

ARP1 contributes to vital cellular networks that drive intracellular transport and mitotic spindle orientation. One important pathway where ARP1 acts is the dynein-mediated transport pathway. Within this network ARP1 partners with the protein dynein to facilitate the movement of vesicles and organelles. Another critical pathway is related to mitotic spindle formation where ARP1 influences the positioning and dynamics of the spindle apparatus ensuring accurate chromosome segregation.

ARP1 possesses connections to neurodegenerative diseases and cancer. Alterations in ARP1 function can lead to impaired axonal transport contributing to conditions like Alzheimer's disease. In the context of cancer ARP1 mutations or dysregulation can disrupt normal cellular division and polarity potentially promoting oncogenesis. The association of ARP1 with p150Glued further links it to neurological diseases as mutations in these proteins can exacerbate cellular dysfunction in neurons.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules.
See full target information ACTR1A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 39:4207-4220 PubMed38727079

2024

Functional mechanism of hypoxia-like conditions mediating resistance to ferroptosis in cervical cancer cells by regulating KDM4A SUMOylation and the SLC7A11/GPX4 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xiong,Puxiang Chen,Ling He,Xiaoshan Chai,Yongjing Zhang,Shujuan Sun
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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