Rabbit Polyclonal ARP2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Xenopus laevis samples. Cited in 24 publications.
Images
Publications
Filter by
- eLife 12:2023N-WASP-dependent branched actin polymerization attenuates B-cell receptor signaling by increasing the molecular density of receptor clusters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnshuman Bhanja,Margaret K Seeley-Fallen,Michelle Lazzaro,Arpita Upadhyaya,Wenxia SongPubMed 38085658
- Cell death & disease 14:5482023The activated CD36-Src axis promotes lung adenocarcinoma cell proliferation and actin remodeling-involved metastasis in high-fat environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Zhong Liu,Bowen Wang,Rui Zhang,Zangshu Wu,Yuxi Huang,Xiaoyang Zhang,Jiaying Zhou,Junbo Yi,Jian Shen,Ming-Yue Li,Ming DongPubMed 37612265
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e227152022Annexin A2 modulates phospholipid membrane composition upstream of Arp2 to control angiogenic sprout initiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTimothy M Sveeggen,Colette A Abbey,Rebecca L Smith,Michael L Salinas,Robert S Chapkin,Kayla J BaylessPubMed 36527391
- Journal of cell science 135:2022Distinct roles of the Chlamydia trachomatis effectors TarP and TmeA in the regulation of formin and Arp2/3 during entry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew D Romero,Rey A CarabeoPubMed 36093837
- Molecular cell 82:2132-2147.e62022Heterochromatic repeat clustering imposes a physical barrier on homologous recombination to prevent chromosomal translocations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIoanna Mitrentsi,Jieqiong Lou,Adèle Kerjouan,John Verigos,Bernardo Reina-San-Martin,Elizabeth Hinde,Evi SoutoglouPubMed 35447083
- Cell research 32:190-2092021Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Wang,Sharad C Paudyal,Yuchen Kang,Mikito Owa,Feng-Xia Liang,Alexander Spektor,Holger Knaut,Irma Sánchez,Brian D DynlachtPubMed 34782749
- The Journal of cell biology 220:2021Hematopoietic progenitors polarize in contact with bone marrow stromal cells in response to SDF1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas Bessy,Adrian Candelas,Benoit Souquet,Khansa Saadallah,Alexandre Schaeffer,Benoit Vianay,Damien Cuvelier,Samy Gobaa,Cecilia Nakid-Cordero,Julien Lion,Jean-Christophe Bories,Nuala Mooney,Thierry Jaffredo,Jerome Larghero,Laurent Blanchoin,Lionel Faivre,Stephane Brunet,Manuel ThéryPubMed 34570198
- International journal of biological sciences 17:1716-17302021NDRG1 regulates Filopodia-induced Colorectal Cancer invasiveness via modulating CDC42 activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBatuer Aikemu,Yanfei Shao,Guang Yang,Junjun Ma,Sen Zhang,Xiao Yang,Hiju Hong,Galiya Yesseyeva,Ling Huang,Hongtao Jia,Chenxing Wang,Lu Zang,Jing Sun,Minhua ZhengPubMed 33994856
- Cancer science 110:3788-38012019Pimozide suppresses cancer cell migration and tumor metastasis through binding to ARPC2, a subunit of the Arp2/3 complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiyeon Choi et. al.PubMed 31571309
- Nature methods 15:909-9122018A high-throughput pipeline for validation of antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKrzysztof Sikorski et. al.PubMed 30377371
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Xenopus laevis | Expected | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis | Dilution info - | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Xenopus laevis | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9000076). Seems to contact the pointed end of the daughter actin filament (PubMed:9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed:29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:17220302, PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).
Alternative names
ARP2, ACTR2, Actin-related protein 2, Actin-like protein 2
Recommended products
Rabbit Polyclonal ARP2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Xenopus laevis samples. Cited in 24 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab47654 are tested in WB. Previous batches were additionally validated in ICC/IF and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab129018.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Arp2 also known as actin-related protein 2 is a subunit of the Arp2/3 complex which plays an important role in nucleating actin filaments. This protein facilitates the formation of branched actin networks important for cellular functions. Arp2 has a mass of approximately 44 kDa and is widely expressed across different tissues especially in regions with significant actin cytoskeletal remodeling such as the cortex of cells.
- Biological function summary
Arp2 acts as a central component within the Arp2/3 complex which regulates actin polymerization and branching. This complex consists of seven subunits and Arp2 in concert with Arp3 and additional regulatory proteins initiates new actin filament branches from pre-existing ones. As a result it is essential for cellular processes like cell movement phagocytosis and maintaining cell shape by controlling the spatial and temporal organization of actin filaments.
- Pathways
The Arp2/3 complex is integral to the actin polymerization pathway and the pathway for cell motility. Arp2 influences the dynamics of filamentous actin interacting with proteins such as Wiskott-Aldrich syndrome protein (WASP) to promote branching. This process affects various cellular activities linked to actin forming connections with the Rho GTPase signaling network which regulates cytoskeletal dynamics and cellular signaling pathways.
- Associated diseases and disorders
Disruptions in the function of the Arp2/3 complex have been associated with Wiskott-Aldrich syndrome and cancer metastasis. Wiskott-Aldrich syndrome linked with mutations in the WASP protein shows impaired actin branching capability due to insufficient Arp2/3 complex activity. Additionally aberrant regulation of actin polymerization in cancer cells leads to enhanced invasive properties implicating Arp2 as a potential factor in the pathology of metastatic progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-Arp2 antibody (ab47654)
All lanes: Western blot - Anti-Arp2 antibody (ab47654) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Lane 5: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 6: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 27 kDa, 45 kDa, 60 kDa
Immunoprecipitation - Anti-Arp2 antibody (ab47654)
Arp2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to Arp2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab47654.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 45kDa: Arp2.All lanes: Immunoprecipitation - Anti-Arp2 antibody (ab47654)
Predicted band size: 45 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody (ab47654)
ICC/IF image of ab47654 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab47654, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293, HepG2 and MCF7 cells.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com