AB47654

Anti-Arp2 antibody

Name: Anti-Arp2 antibody (ab47654) | Abcam
Brand: Abcam Limited
SKU: AB47654
Price: 160 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(25 Publications)

Rabbit Polyclonal ARP2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Xenopus laevis samples. Cited in 25 publications.

View Alternative Names

ARP2, ACTR2, Actin-related protein 2, Actin-like protein 2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody (AB47654)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody (AB47654)

ICC/IF image of ab47654 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab47654, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293, HepG2 and MCF7 cells.

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Immunoprecipitation - Anti-Arp2 antibody (AB47654)

Arp2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to Arp2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab47654.
Secondary : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band : 45kDa : Arp2.

All lanes:

Immunoprecipitation - Anti-Arp2 antibody (ab47654)

Predicted band size: 45 kDa

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Project

Western blot - Anti-Arp2 antibody (AB47654)

All lanes:

Western blot - Anti-Arp2 antibody (ab47654) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 3:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 5:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 45 kDa

Observed band size: 27 kDa,45 kDa,60 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Xenopus laevis, Mouse

Applications

IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Replenishment batches of our polyclonal antibody, ab47654 are tested in WB. Previous batches were additionally validated in ICC/IF and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab129018.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arp2 also known as actin-related protein 2 is a subunit of the Arp2/3 complex which plays an important role in nucleating actin filaments. This protein facilitates the formation of branched actin networks important for cellular functions. Arp2 has a mass of approximately 44 kDa and is widely expressed across different tissues especially in regions with significant actin cytoskeletal remodeling such as the cortex of cells.

Biological function summary

Arp2 acts as a central component within the Arp2/3 complex which regulates actin polymerization and branching. This complex consists of seven subunits and Arp2 in concert with Arp3 and additional regulatory proteins initiates new actin filament branches from pre-existing ones. As a result it is essential for cellular processes like cell movement phagocytosis and maintaining cell shape by controlling the spatial and temporal organization of actin filaments.

Pathways

The Arp2/3 complex is integral to the actin polymerization pathway and the pathway for cell motility. Arp2 influences the dynamics of filamentous actin interacting with proteins such as Wiskott-Aldrich syndrome protein (WASP) to promote branching. This process affects various cellular activities linked to actin forming connections with the Rho GTPase signaling network which regulates cytoskeletal dynamics and cellular signaling pathways.

Disruptions in the function of the Arp2/3 complex have been associated with Wiskott-Aldrich syndrome and cancer metastasis. Wiskott-Aldrich syndrome linked with mutations in the WASP protein shows impaired actin branching capability due to insufficient Arp2/3 complex activity. Additionally aberrant regulation of actin polymerization in cancer cells leads to enhanced invasive properties implicating Arp2 as a potential factor in the pathology of metastatic progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9000076). Seems to contact the pointed end of the daughter actin filament (PubMed : 9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed : 29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 17220302, PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).

See full target information ACTR2

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed38085658

2023

N-WASP-dependent branched actin polymerization attenuates B-cell receptor signaling by increasing the molecular density of receptor clusters.

Applications

Unspecified application

Species

Unspecified reactive species

Anshuman Bhanja,Margaret K Seeley-Fallen,Michelle Lazzaro,Arpita Upadhyaya,Wenxia Song

eLife 12: PubMed37768710

2023

Hierarchical morphogenesis of swallowtail butterfly wing scale nanostructures.

Applications

Unspecified application

Species

Unspecified reactive species

Kwi Shan Seah,Vinodkumar Saranathan

Cell death & disease 14:548 PubMed37612265

2023

The activated CD36-Src axis promotes lung adenocarcinoma cell proliferation and actin remodeling-involved metastasis in high-fat environment.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Zhong Liu,Bowen Wang,Rui Zhang,Zangshu Wu,Yuxi Huang,Xiaoyang Zhang,Jiaying Zhou,Junbo Yi,Jian Shen,Ming-Yue Li,Ming Dong

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22715 PubMed36527391

2022

Annexin A2 modulates phospholipid membrane composition upstream of Arp2 to control angiogenic sprout initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy M Sveeggen,Colette A Abbey,Rebecca L Smith,Michael L Salinas,Robert S Chapkin,Kayla J Bayless

Journal of cell science 135: PubMed36093837

2022

Distinct roles of the Chlamydia trachomatis effectors TarP and TmeA in the regulation of formin and Arp2/3 during entry.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew D Romero,Rey A Carabeo

Molecular cell 82:2132-2147.e6 PubMed35447083

2022

Heterochromatic repeat clustering imposes a physical barrier on homologous recombination to prevent chromosomal translocations.

Applications

Unspecified application

Species

Unspecified reactive species

Ioanna Mitrentsi,Jieqiong Lou,Adèle Kerjouan,John Verigos,Bernardo Reina-San-Martin,Elizabeth Hinde,Evi Soutoglou

Cell research 32:190-209 PubMed34782749

2021

Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Sharad C Paudyal,Yuchen Kang,Mikito Owa,Feng-Xia Liang,Alexander Spektor,Holger Knaut,Irma Sánchez,Brian D Dynlacht

The Journal of cell biology 220: PubMed34570198

2021

Hematopoietic progenitors polarize in contact with bone marrow stromal cells in response to SDF1.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Bessy,Adrian Candelas,Benoit Souquet,Khansa Saadallah,Alexandre Schaeffer,Benoit Vianay,Damien Cuvelier,Samy Gobaa,Cecilia Nakid-Cordero,Julien Lion,Jean-Christophe Bories,Nuala Mooney,Thierry Jaffredo,Jerome Larghero,Laurent Blanchoin,Lionel Faivre,Stephane Brunet,Manuel Théry

International journal of biological sciences 17:1716-1730 PubMed33994856

2021

NDRG1 regulates Filopodia-induced Colorectal Cancer invasiveness via modulating CDC42 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Batuer Aikemu,Yanfei Shao,Guang Yang,Junjun Ma,Sen Zhang,Xiao Yang,Hiju Hong,Galiya Yesseyeva,Ling Huang,Hongtao Jia,Chenxing Wang,Lu Zang,Jing Sun,Minhua Zheng

Cancer science 110:3788-3801 PubMed31571309

2019

Pimozide suppresses cancer cell migration and tumor metastasis through binding to ARPC2, a subunit of the Arp2/3 complex.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyeon Choi,Yu-Jin Lee,Yae Jin Yoon,Cheol-Hee Kim,Seung-Jin Park,Seon-Young Kim,Nam Doo Kim,Dong Cho Han,Byoung-Mog Kwon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Arp2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody (AB47654)

Immunoprecipitation - Anti-Arp2 antibody (AB47654)

Western blot - Anti-Arp2 antibody (AB47654)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (25)

Product promise