Anti-Arp2 antibody [EPR7979]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Arp2 antibody [EPR7979] (AB129018)

Intracellular flow cytometric analysis of permeabilized MCF-7 cells using ab129018 at 1/10 (red) or a rabbit IgG (negative) (green)

• WB

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Western blot - Anti-Arp2 antibody [EPR7979] (AB129018)

All lanes:

Western blot - Anti-Arp2 antibody [EPR7979] (ab129018) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HUVEC cell lysate at 10 µg

Lane 3:

MCF-7 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 45 kDa

Observed band size: 37 kDa

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• OI-RD Scanning

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OI-RD Scanning - Anti-Arp2 antibody [EPR7979] (AB129018)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

• WB

CiteAb

Western blot - Anti-Arp2 antibody [EPR7979] (AB129018)

Arp2 western blot using anti-Arp2 antibody [EPR7979] ab129018. Publication image and figure legend from Mehedi, M., McCarty, T., et al., 2016, PLoS Pathog, PubMed 27926942.

ab129018 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab129018 please see the product overview.

ARP2 knockdown in human respiratory epithelial A549 cells.(A) Knockdown of ARP2 protein expression. Replicate monolayers of A549 cells were transfected with siARP2, siControl, or no siRNA, as indicated. At 48 hr post-transfection, one set of monolayers was harvested. The others were infected with RSV-GFP (MOI = 1) or mock-infected, and harvested at 24, 48, and 72 hpi, as indicated. The cells were processed for Western blot. ARP2 protein was detected using a primary rabbit mAb and an anti-rabbit IgG IRDye800 secondary Ab. Alpha-tubulin, as a loading control, was detected with a primary mouse mAb and an anti-mouse IgG IRDye680 secondary Ab. Bound antibodies were visualized by infrared fluorescence. One representative of four independent experiments is shown. (B) Knockdown of ARP2 mRNA expression. A549 cells were transfected with siARP2, siControl, or no siRNA, as indicated. Sets of monolayers were harvested 24 and 48 hr post-transfection. The remaining monolayers were infected with RSV-GFP (MOI = 1) or mock-infected, and harvested at 24, 48, and 72 hpi, as indicated. Total cell-associated ARP2 mRNA was quantified by real-time PCR using a TaqMan assay for ARP2. 18S ribosomal RNA was used as an endogenous control for normalization of each reaction, and the values of ARP2 expression are shown as fold-change relative to the no-siRNA control 24 hr post-transfection. Each sample was tested in quadruplicate by TaqMan assay and the averages are presented. (C) ARP2 knockdown did not reduce cell viability. A549 cells were transfected with siARP2, siControl or no siRNA for 48 hr. Cell viability was compared using alamarBlue and expressed relative to the no-siRNA control. Data from three independent experiments, each done in triplicate were combined for analysis. Error bars show standard deviation (SD).

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