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AB248263

Anti-Arp2 antibody [EPR7979] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ARP2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ARP2, ACTR2, Actin-related protein 2, Actin-like protein 2

3 Images
Flow Cytometry (Intracellular) - Anti-Arp2 antibody [EPR7979] - BSA and Azide free (AB248263)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Arp2 antibody [EPR7979] - BSA and Azide free (AB248263)

This data was developed using ab129018, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized MCF-7 cells using ab129018 at 1/10 (red) or a rabbit IgG (negative) (green)

Western blot - Anti-Arp2 antibody [EPR7979] - BSA and Azide free (AB248263)
  • WB

Unknown

Western blot - Anti-Arp2 antibody [EPR7979] - BSA and Azide free (AB248263)

This data was developed using ab129018, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Arp2 antibody [EPR7979] (<a href='/en-us/products/primary-antibodies/arp2-antibody-epr7979-ab129018'>ab129018</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HUVEC cell lysate at 10 µg

Lane 3:

MCF-7 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 45 kDa

Observed band size: 37 kDa

false

OI-RD Scanning - Anti-Arp2 antibody [EPR7979] - BSA and Azide free (AB248263)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Arp2 antibody [EPR7979] - BSA and Azide free (AB248263)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7979

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248263 is the carrier-free version of ab129018.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arp2 also known as actin-related protein 2 is a subunit of the Arp2/3 complex which plays an important role in nucleating actin filaments. This protein facilitates the formation of branched actin networks important for cellular functions. Arp2 has a mass of approximately 44 kDa and is widely expressed across different tissues especially in regions with significant actin cytoskeletal remodeling such as the cortex of cells.
Biological function summary

Arp2 acts as a central component within the Arp2/3 complex which regulates actin polymerization and branching. This complex consists of seven subunits and Arp2 in concert with Arp3 and additional regulatory proteins initiates new actin filament branches from pre-existing ones. As a result it is essential for cellular processes like cell movement phagocytosis and maintaining cell shape by controlling the spatial and temporal organization of actin filaments.

Pathways

The Arp2/3 complex is integral to the actin polymerization pathway and the pathway for cell motility. Arp2 influences the dynamics of filamentous actin interacting with proteins such as Wiskott-Aldrich syndrome protein (WASP) to promote branching. This process affects various cellular activities linked to actin forming connections with the Rho GTPase signaling network which regulates cytoskeletal dynamics and cellular signaling pathways.

Disruptions in the function of the Arp2/3 complex have been associated with Wiskott-Aldrich syndrome and cancer metastasis. Wiskott-Aldrich syndrome linked with mutations in the WASP protein shows impaired actin branching capability due to insufficient Arp2/3 complex activity. Additionally aberrant regulation of actin polymerization in cancer cells leads to enhanced invasive properties implicating Arp2 as a potential factor in the pathology of metastatic progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9000076). Seems to contact the pointed end of the daughter actin filament (PubMed : 9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed : 29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 17220302, PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).
See full target information ACTR2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular gastroenterology and hepatology 12:395-425 PubMed33676046

2021

Helicobacter pylori-Induced Rev-erbα Fosters Gastric Bacteria Colonization by Impairing Host Innate and Adaptive Defense.

Applications

Unspecified application

Species

Unspecified reactive species

Fang-Yuan Mao,Yi-Pin Lv,Chuan-Jie Hao,Yong-Sheng Teng,Yu-Gang Liu,Ping Cheng,Shi-Ming Yang,Weisan Chen,Tao Liu,Quan-Ming Zou,Rui Xie,Jing-Yu Xu,Yuan Zhuang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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