Anti-Arp2 antibody [EPR7980]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp2 antibody [EPR7980] (AB128934)

ab128934, at a 1/100 dilution, staining Arp2 in paraffin-embedded human uterus tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

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Western blot - Anti-Arp2 antibody [EPR7980] (AB128934)

All lanes:

Western blot - Anti-Arp2 antibody [EPR7980] (ab128934) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HUVEC cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 45 kDa

Observed band size: 37 kDa

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• OI-RD Scanning

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OI-RD Scanning - Anti-Arp2 antibody [EPR7980] (AB128934)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

• WB

CiteAb

Western blot - Anti-Arp2 antibody [EPR7980] (AB128934)

Arp2 western blot using anti-Arp2 antibody [EPR7980] ab128934. Publication image and figure legend from Kahr, W. H., Pluthero, F. G., et al., 2017, Nat Commun, PubMed 28368018.

ab128934 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab128934 please see the product overview.

ARPC1B and Arp2/3 complex are deficient in ARPC1B mutant platelets.(a) Crystal structure of mammalian Arp2/3 showing relative location of component proteins from the protein data bank (PDB1K8K, http : //www.rcsb.org/pdb/explore/explore.do?structureId=1K8K; Robinson et al.16). (b) Immunoblot analysis of platelet lysates showed ARPC1B to be absent in Patient 1 (P1) and greatly reduced in Patients 2 and 3 (P2, P3) compared to normal (N), while levels of ARPC2, ARPC3, ARPC5, ARP2 and ARP3 were normal (gamma tubulin used as loading control, see Supplementary Fig. 3a). (c) Platelet ARPC1A was increased in all three patients relative to normal, while WASP expression was equivalent (GAPDH used as loading control). (d) Immunoblot analysis of a normal platelet lysate after native gel electrophoresis showed a band corresponding to Arp2/3 complex detected by probing for ARPC1B (shown) or other Arp2/3 components (Supplementary Fig. 3d). This band was resolved on a second dimension SDS–PAGE gel, and immunoblot confirmed the presence of Arp2/3 components (ARPC1B, ARPC2, ARP2 and ARP3 shown). (e) Immunoblot of platelet lysates after native gel electrophoresis for ARPC5 (left) showed a greatly reduced level of Arp2/3 in Patient 1 (P1) platelets relative to normal (N). ARPC1A (right) was detected in the Arp2/3 complex in Patient 1 (P1) but not in normal (N) platelet lysate (native GAPDH tetramer used as loading control).

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