AB49674

Anti-Arp2 antibody [FMS96]

Name: Anti-Arp2 antibody [FMS96] (ab49674) | Abcam
Brand: Abcam Limited
SKU: AB49674
Price: 510 USD
Availability: InStock

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(18 Publications)

Mouse Monoclonal ARP2 antibody. Suitable for WB, I-ELISA, ICC/IF and reacts with Mouse, Rat, Human, Dog samples. Cited in 18 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ACTR2.

View Alternative Names

ARP2, ACTR2, Actin-related protein 2, Actin-like protein 2

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody [FMS96] (AB49674)

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody [FMS96] (AB49674)

Immunofluorescence analysis of dendritic cells infected with HIV, staining Arp2 (blue) with ab49674 at 5 mg/ml.

Image from Aggarwal A et al., PLoS Pathog. 2012 Jun;8(6):e1002762. Epub 2012 Jun 7. Fig 4.; doi:10.1371/journal.ppat.1002762; June 7, 2012, PLoS Pathog 8(6): e1002762.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody [FMS96] (AB49674)

C2C12 cells were incubated with ab49674 at 10 µg/ml followed by Goat Anti Mouse IgG Alexa-488 (green staining). The red staining is obtained by staining actin with Alexa-546 phalloidin.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

FMS96

Isotype

IgG1

Carrier free

Reacts with

Mouse, Dog, Rat, Human

Applications

I-ELISA, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ACTR2.

P61160

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arp2 also known as actin-related protein 2 is a subunit of the Arp2/3 complex which plays an important role in nucleating actin filaments. This protein facilitates the formation of branched actin networks important for cellular functions. Arp2 has a mass of approximately 44 kDa and is widely expressed across different tissues especially in regions with significant actin cytoskeletal remodeling such as the cortex of cells.

Biological function summary

Arp2 acts as a central component within the Arp2/3 complex which regulates actin polymerization and branching. This complex consists of seven subunits and Arp2 in concert with Arp3 and additional regulatory proteins initiates new actin filament branches from pre-existing ones. As a result it is essential for cellular processes like cell movement phagocytosis and maintaining cell shape by controlling the spatial and temporal organization of actin filaments.

Pathways

The Arp2/3 complex is integral to the actin polymerization pathway and the pathway for cell motility. Arp2 influences the dynamics of filamentous actin interacting with proteins such as Wiskott-Aldrich syndrome protein (WASP) to promote branching. This process affects various cellular activities linked to actin forming connections with the Rho GTPase signaling network which regulates cytoskeletal dynamics and cellular signaling pathways.

Disruptions in the function of the Arp2/3 complex have been associated with Wiskott-Aldrich syndrome and cancer metastasis. Wiskott-Aldrich syndrome linked with mutations in the WASP protein shows impaired actin branching capability due to insufficient Arp2/3 complex activity. Additionally aberrant regulation of actin polymerization in cancer cells leads to enhanced invasive properties implicating Arp2 as a potential factor in the pathology of metastatic progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9000076). Seems to contact the pointed end of the daughter actin filament (PubMed : 9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed : 29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 17220302, PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).

See full target information ACTR2

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Biochimica et biophysica acta. Molecular cell research 1871:119674 PubMed38242328

2024

AKAP2-anchored extracellular signal-regulated kinase 1 (ERK1) regulates cardiac myofibroblast migration.

Applications

Unspecified application

Species

Unspecified reactive species

Marion Delaunay,Aleksandra Paterek,Ivan Gautschi,Greta Scherler,Dario Diviani

Biophysical journal 122:3704-3721 PubMed37301982

2023

Chiral growth of adherent filopodia.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhong Li,Wen-Lu Chung,Michael M Kozlov,Ohad Medalia,Benjamin Geiger,Alexander D Bershadsky

Cell 185:4507-4525.e18 PubMed36356582

2022

An ancestral mycobacterial effector promotes dissemination of infection.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph W Saelens,Mollie I Sweeney,Gopinath Viswanathan,Ana María Xet-Mull,Kristen L Jurcic Smith,Dana M Sisk,Daniel D Hu,Rachel M Cronin,Erika J Hughes,W Jared Brewer,Jörn Coers,Matthew M Champion,Patricia A Champion,Craig B Lowe,Clare M Smith,Sunhee Lee,Jason E Stout,David M Tobin

Nature communications 13:5529 PubMed36130971

2022

Differential dysregulation of granule subsets in WASH-deficient neutrophil leukocytes resulting in inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer L Johnson,Elsa Meneses-Salas,Mahalakshmi Ramadass,Jlenia Monfregola,Farhana Rahman,Raquel Carvalho Gontijo,William B Kiosses,Kersi Pestonjamasp,Dale Allen,Jinzhong Zhang,Douglas G Osborne,Yanfang Peipei Zhu,Nathan Wineinger,Kasra Askari,Danni Chen,Juan Yu,Scott C Henderson,Catherine C Hedrick,Matilde Valeria Ursini,Sergio Grinstein,Daniel D Billadeau,Sergio D Catz

Progress in neurobiology 205:102122 PubMed34284000

2021

c-Abl regulates a synaptic plasticity-related transcriptional program involved in memory and learning.

Applications

Unspecified application

Species

Unspecified reactive species

Adrián González-Martín,Tomás Moyano,Daniela A Gutiérrez,Franciso J Carvajal,Waldo Cerpa,Jonathan G Hanley,Rodrigo A Gutiérrez,Alejandra R Álvarez

iScience 24:102724 PubMed34337357

2021

Microscopic image-based covariation network analysis for actin scaffold-modified insulin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiyuki Noguchi,Fumi Kano,Nobuhiko Maiya,Chisako Iwamoto,Shoko Yamasaki,Yosuke Otsubo,Daiki Nakatsu,Rina Kunishige,Masayuki Murata

The EMBO journal 39:e104926 PubMed32510692

2020

Direct binding of polymeric GBP1 to LPS disrupts bacterial cell envelope functions.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam Kutsch,Linda Sistemich,Cammie F Lesser,Marcia B Goldberg,Christian Herrmann,Jörn Coers

iScience 15:274-281 PubMed31096079

2019

Centrosomal Actin Assembly Is Required for Proper Mitotic Spindle Formation and Chromosome Congression.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias Plessner,Julian Knerr,Robert Grosse

PLoS genetics 13:e1007078 PubMed29136647

2017

Katanin-like 2 (KATNAL2) functions in multiple aspects of haploid male germ cell development in the mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica E M Dunleavy,Hidenobu Okuda,Anne E O'Connor,D Jo Merriner,Liza O'Donnell,Duangporn Jamsai,Martin Bergmann,Moira K O'Bryan

Scientific reports 7:960 PubMed28424461

2017

A lack of coordination between sister-chromatids segregation and cytokinesis in the oocytes of B6.Y (XY) sex-reversed female mice.

Applications

ICC/IF

Species

Mouse

Jia-Qiao Zhu,Seang Lin Tan,Teruko Taketo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Arp2 antibody [FMS96]

Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody [FMS96] (AB49674)

Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody [FMS96] (AB49674)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise