AB119766

Anti-Arp2 (phospho T237 + T238) antibody

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(3 Publications)

Rabbit Polyclonal ARP2 phospho T237 + T238 antibody. Suitable for ICC, WB and reacts with Rat, Human samples. Cited in 3 publications.

View Alternative Names

ARP2, ACTR2, Actin-related protein 2, Actin-like protein 2

2 Images

Immunocytochemistry - Anti-Arp2 (phospho T237 + T238) antibody (AB119766)

ICC

Unknown

Immunocytochemistry - Anti-Arp2 (phospho T237 + T238) antibody (AB119766)

ab119766 at 1/100 dilution staining Arp2 (phospho T237 + T238) in rat PC12 cells differentiated with NGF (right).
Detection used an appropriate secondary antibody conjugated to Cy3.

Western blot - Anti-Arp2 (phospho T237 + T238) antibody (AB119766)

WB

Unknown

Western blot - Anti-Arp2 (phospho T237 + T238) antibody (AB119766)

All lanes:

Western blot - Anti-Arp2 (phospho T237 + T238) antibody (ab119766) at 1/1000 dilution

Lane 1:

A431 cells treated with Calyculin A(100 nM) for 30 min

Lane 2:

A431 cells treated with Calyculin A(100 nM) for 30 min and treated with lambda phosphatase

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

ICC, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Fish": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Do not aliquot.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arp2 also known as actin-related protein 2 is a subunit of the Arp2/3 complex which plays an important role in nucleating actin filaments. This protein facilitates the formation of branched actin networks important for cellular functions. Arp2 has a mass of approximately 44 kDa and is widely expressed across different tissues especially in regions with significant actin cytoskeletal remodeling such as the cortex of cells.

Biological function summary

Arp2 acts as a central component within the Arp2/3 complex which regulates actin polymerization and branching. This complex consists of seven subunits and Arp2 in concert with Arp3 and additional regulatory proteins initiates new actin filament branches from pre-existing ones. As a result it is essential for cellular processes like cell movement phagocytosis and maintaining cell shape by controlling the spatial and temporal organization of actin filaments.

Pathways

The Arp2/3 complex is integral to the actin polymerization pathway and the pathway for cell motility. Arp2 influences the dynamics of filamentous actin interacting with proteins such as Wiskott-Aldrich syndrome protein (WASP) to promote branching. This process affects various cellular activities linked to actin forming connections with the Rho GTPase signaling network which regulates cytoskeletal dynamics and cellular signaling pathways.

Disruptions in the function of the Arp2/3 complex have been associated with Wiskott-Aldrich syndrome and cancer metastasis. Wiskott-Aldrich syndrome linked with mutations in the WASP protein shows impaired actin branching capability due to insufficient Arp2/3 complex activity. Additionally aberrant regulation of actin polymerization in cancer cells leads to enhanced invasive properties implicating Arp2 as a potential factor in the pathology of metastatic progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9000076). Seems to contact the pointed end of the daughter actin filament (PubMed : 9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed : 29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 17220302, PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).

See full target information ACTR2 pT237 + T238

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 133: PubMed32107292

2020

Direct interaction between CEP85 and STIL mediates PLK4-driven directed cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Liu,Jaeyoun Kim,Reuben Philip,Vaishali Sridhar,Megha Chandrashekhar,Jason Moffat,Mark van Breugel,Laurence Pelletier

PLoS pathogens 14:e1007005 PubMed29668757

2018

Actin polymerization in the endosomal pathway, but not on the Coxiella-containing vacuole, is essential for pathogen growth.

Applications

Unspecified application

Species

Unspecified reactive species

Heather E Miller,Charles L Larson,Robert A Heinzen

Journal of immunology (Baltimore, Md. : 1950) 199:3176-3186 PubMed28947541

2017

Aging-Impaired Filamentous Actin Polymerization Signaling Reduces Alveolar Macrophage Phagocytosis of Bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Zhigang Li,Yang Jiao,Erica K Fan,Melanie J Scott,Yuehua Li,Song Li,Timothy R Billiar,Mark A Wilson,Xueyin Shi,Jie Fan

View all publications

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Product promise

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