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AB249046

Anti-Arp3 antibody [EPR10428(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal ARP3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Human samples.

View Alternative Names

ARP3, ACTR3, Actin-related protein 3, Actin-like protein 3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10428(B)] - BSA and Azide free (AB249046)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10428(B)] - BSA and Azide free (AB249046)

This data was developed using ab151729, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Arp3 with ab151729 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10428(B)] - BSA and Azide free (AB249046)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp3 antibody [EPR10428(B)] - BSA and Azide free (AB249046)

This data was developed using ab151729, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Arp3 with ab151729 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-Arp3 antibody [EPR10428(B)] - BSA and Azide free (AB249046)
  • WB

Unknown

Western blot - Anti-Arp3 antibody [EPR10428(B)] - BSA and Azide free (AB249046)

This data was developed using ab151729, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Arp3 antibody [EPR10428(B)] (<a href='/en-us/products/primary-antibodies/arp3-antibody-epr10428b-ab151729'>ab151729</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 47 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10428(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab249046 is the carrier-free version of ab151729.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arp3 also known as Actin-related protein 3 is an important component of the human cellular cytoskeleton machinery. It has a molecular mass of approximately 47 kDa and is ubiquitously expressed across various tissues. Arp3 participates in the nucleation and branching of actin filaments acting mechanically to control the structural organization of the cytoskeleton. This protein is highly conserved across species which shows its important role in fundamental cellular processes.
Biological function summary

Arp3 plays a significant role in cellular motility and morphology. It is a part of the Arp2/3 complex which consists of seven subunits that assemble to promote actin nucleation. This complex is central to the formation of branched actin networks which are necessary for processes such as cell movement endocytosis and vesicle trafficking. Through its mechanical action within the Arp2/3 complex Arp3 influences the dynamic rearrangement of the cytoskeleton necessary for cellular responses to external stimuli.

Pathways

Arp3 functions within the Wiskott-Aldrich syndrome protein (WASP) pathway and the Rho family of GTPases pathway. These pathways are important for regulating the actin cytoskeleton dynamics. The WASP pathway activates Arp2/3 complex triggering branched actin filament assembly. This process interacts with proteins like Cdc42 a member of the Rho GTPase family which further modulates cytoskeletal organization. Through these interactions Arp3 assists in coordinating actin-based processes critical to cell shape and movement.

Arp3 links to immune system problems and cancer progression. Mutations or dysregulation of the Arp2/3 complex including Arp3 can lead to immune deficiencies where cellular movement is impaired such as seen in Wiskott-Aldrich Syndrome. Furthermore aberrant Arp3 activity associates with cancer metastasis where altered cell motility plays a role in tumor spread. Connected proteins such as N-WASP often display interaction within these pathological contexts reinforcing Arp3's relevance in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9000076). Seems to contact the pointed end of the daughter actin filament (PubMed : 9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed : 29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 17220302, PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947). Plays a role in ciliogenesis (PubMed : 20393563).
See full target information ACTR3
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Product promise

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For full details, please see our Terms & Conditions

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