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AB49671

Anti-Arp3 antibody [FMS338]

5

(2 Reviews)

|

(32 Publications)

Mouse Monoclonal ARP3 antibody. Suitable for WB, I-ELISA, ICC/IF and reacts with Mouse, Rat, Human, Dog, African green monkey samples. Cited in 32 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ACTR3.

View Alternative Names

ARP3, ACTR3, Actin-related protein 3, Actin-like protein 3

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Arp3 antibody [FMS338] (AB49671)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Arp3 antibody [FMS338] (AB49671)

C2C12 cells were incubated with ab49671 at 10µg/ml followed by Alexa-488 conjugated secondary antibody(green staining). The red stain is obtained by staining actin with Alexa-456 phalloidin.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

FMS338

Isotype

IgG2b

Carrier free

No

Reacts with

Mouse, Rat, Dog, African green monkey, Human

Applications

WB, ICC/IF, I-ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ACTR3.

P61158

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Alternative Products

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p>Array: Use at an assay dependent dilution.</p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p>Array: Use at an assay dependent dilution.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p>Array: Use at an assay dependent dilution.</p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Dog": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p>Array: Use at an assay dependent dilution.</p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Arp3 also known as Actin-related protein 3 is an important component of the human cellular cytoskeleton machinery. It has a molecular mass of approximately 47 kDa and is ubiquitously expressed across various tissues. Arp3 participates in the nucleation and branching of actin filaments acting mechanically to control the structural organization of the cytoskeleton. This protein is highly conserved across species which shows its important role in fundamental cellular processes.
Biological function summary

Arp3 plays a significant role in cellular motility and morphology. It is a part of the Arp2/3 complex which consists of seven subunits that assemble to promote actin nucleation. This complex is central to the formation of branched actin networks which are necessary for processes such as cell movement endocytosis and vesicle trafficking. Through its mechanical action within the Arp2/3 complex Arp3 influences the dynamic rearrangement of the cytoskeleton necessary for cellular responses to external stimuli.

Pathways

Arp3 functions within the Wiskott-Aldrich syndrome protein (WASP) pathway and the Rho family of GTPases pathway. These pathways are important for regulating the actin cytoskeleton dynamics. The WASP pathway activates Arp2/3 complex triggering branched actin filament assembly. This process interacts with proteins like Cdc42 a member of the Rho GTPase family which further modulates cytoskeletal organization. Through these interactions Arp3 assists in coordinating actin-based processes critical to cell shape and movement.

Arp3 links to immune system problems and cancer progression. Mutations or dysregulation of the Arp2/3 complex including Arp3 can lead to immune deficiencies where cellular movement is impaired such as seen in Wiskott-Aldrich Syndrome. Furthermore aberrant Arp3 activity associates with cancer metastasis where altered cell motility plays a role in tumor spread. Connected proteins such as N-WASP often display interaction within these pathological contexts reinforcing Arp3's relevance in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9000076). Seems to contact the pointed end of the daughter actin filament (PubMed : 9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed : 29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 17220302, PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947). Plays a role in ciliogenesis (PubMed : 20393563).
See full target information ACTR3
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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:27213 PubMed39516580

2024

Rac1 inhibition regenerates wounds in mouse fetuses via altered actin dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Kento Takaya,Yuka Imbe,Qi Wang,Keisuke Okabe,Shigeki Sakai,Noriko Aramaki-Hattori,Kazuo Kishi

Cell death & disease 14:548 PubMed37612265

2023

The activated CD36-Src axis promotes lung adenocarcinoma cell proliferation and actin remodeling-involved metastasis in high-fat environment.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Zhong Liu,Bowen Wang,Rui Zhang,Zangshu Wu,Yuxi Huang,Xiaoyang Zhang,Jiaying Zhou,Junbo Yi,Jian Shen,Ming-Yue Li,Ming Dong

Proceedings of the National Academy of Sciences of the United States of America 120:e2215011120 PubMed36917665

2023

The WAVE complex drives the morphogenesis of the photoreceptor outer segment cilium.

Applications

Unspecified application

Species

Unspecified reactive species

William J Spencer,Nicholas F Schneider,Tylor R Lewis,Carson M Castillo,Nikolai P Skiba,Vadim Y Arshavsky

Proceedings of the National Academy of Sciences of the United States of America 120:e2213272120 PubMed36787367

2023

Cytoskeletal association of ATP citrate lyase controls the mechanodynamics of macropinocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph Puccini,Jia Wei,Liang Tong,Dafna Bar-Sagi

Nature 611:365-373 PubMed36323783

2022

Extracellular fluid viscosity enhances cell migration and cancer dissemination.

Applications

Unspecified application

Species

Unspecified reactive species

Kaustav Bera,Alexander Kiepas,Inês Godet,Yizeng Li,Pranav Mehta,Brent Ifemembi,Colin D Paul,Anindya Sen,Selma A Serra,Konstantin Stoletov,Jiaxiang Tao,Gabriel Shatkin,Se Jong Lee,Yuqi Zhang,Adrianna Boen,Panagiotis Mistriotis,Daniele M Gilkes,John D Lewis,Chen-Ming Fan,Andrew P Feinberg,Miguel A Valverde,Sean X Sun,Konstantinos Konstantopoulos

Pathogens (Basel, Switzerland) 11: PubMed35056004

2022

Embedding of HIV Egress within Cortical F-Actin.

Applications

Unspecified application

Species

Unspecified reactive species

Anupriya Aggarwal,Alberto Ospina Stella,Catherine C Henry,Kedar Narayan,Stuart G Turville

PLoS biology 19:e3001399 PubMed34898601

2021

Poststroke dendritic arbor regrowth requires the actin nucleator Cobl.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Ji,Dennis Koch,Jule González Delgado,Madlen Günther,Otto W Witte,Michael M Kessels,Christiane Frahm,Britta Qualmann

iScience 24:102724 PubMed34337357

2021

Microscopic image-based covariation network analysis for actin scaffold-modified insulin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiyuki Noguchi,Fumi Kano,Nobuhiko Maiya,Chisako Iwamoto,Shoko Yamasaki,Yosuke Otsubo,Daiki Nakatsu,Rina Kunishige,Masayuki Murata

Genes & development 34:1619-1636 PubMed33122293

2020

Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra M Pinzaru,Mike Kareh,Noa Lamm,Eros Lazzerini-Denchi,Anthony J Cesare,Agnel Sfeir

Frontiers in cell and developmental biology 8:592628 PubMed33195261

2020

G Protein-Coupled Estrogen Receptor Regulates Actin Cytoskeleton Dynamics to Impair Cell Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Dariusz Lachowski,Ernesto Cortes,Carlos Matellan,Alistair Rice,David A Lee,Stephen D Thorpe,Armando E Del Río Hernández
View all publications
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Product promise

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