AB227082

Anti-ARPC1B antibody

Name: Anti-ARPC1B antibody (ab227082) | Abcam
Brand: Abcam Limited
SKU: AB227082
Price: 510 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal ARPC1B antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ARPC1B.

View Alternative Names

ARC41, ARPC1B, Actin-related protein 2/3 complex subunit 1B, Arp2/3 complex 41 kDa subunit, p41-ARC

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC1B antibody (AB227082)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC1B antibody (AB227082)

Paraffin-embedded human lung carcinoma tissue stained for ARPC1B with ab227082 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ARPC1B antibody (AB227082)

All lanes:

Western blot - Anti-ARPC1B antibody (ab227082) at 1/500 dilution

All lanes:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Predicted band size: 41 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ARPC1B. The exact immunogen used to generate this antibody is proprietary information.

O15143

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 10% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARPC1B also known as Actin-related protein 2/3 complex subunit 1B weighs around 41 kDa. This protein forms a part of the Arp2/3 complex which is instrumental in actin filament branching and growth. Researchers have detected ARPC1B expression in various tissues including blood cells and the skin highlighting its diverse functional roles in different cell types.

Biological function summary

ARPC1B contributes significantly to cell movement and shape maintenance. The protein acts as a component of the Arp2/3 complex which always requires additional nucleation factors to perform its function. This complex drives the reorganization of the actin cytoskeleton influencing cellular processes like migration and phagocytosis. It modulates the interaction of actin with branching networks thereby ensuring dynamic cellular adaptability to different environments.

Pathways

ARPC1B plays a critical role in the regulation of actin cytoskeleton and focal adhesion pathways. Scientists have associated ARPC1B with the WASP and WAVE proteins which stimulate actin assembly. These interactions enable ARPC1B to facilitate precise signaling pathways important for cell migration and immune responses. The protein’s participation in these pathways illustrates its key function in maintaining cytoskeletal integrity and cellular communication.

ARPC1B has connections to severe combined immunodeficiency and Wiskott-Aldrich syndrome. These conditions often manifest through mutations affecting the actin cytoskeleton. ARPC1B’s relationship with the WASP protein highlights its involvement in regulating immune cell function and signaling. For individuals with deficiencies in ARPC1B these immune complications underline the importance of understanding its function in therapeutic contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 11741539, PubMed : 9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 11741539, PubMed : 9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).

See full target information ARPC1B

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7529 PubMed36477027

2022

Phosphoproteomic analysis of neoadjuvant breast cancer suggests that increased sensitivity to paclitaxel is driven by CDK4 and filamin A.

Applications

Unspecified application

Species

Unspecified reactive species

S Mouron,M J Bueno,A Lluch,L Manso,I Calvo,J Cortes,J A Garcia-Saenz,M Gil-Gil,N Martinez-Janez,J V Apala,E Caleiras,Pilar Ximénez-Embún,J Muñoz,L Gonzalez-Cortijo,R Murillo,R Sánchez-Bayona,J M Cejalvo,G Gómez-López,C Fustero-Torre,S Sabroso-Lasa,N Malats,M Martinez,A Moreno,D Megias,M Malumbres,R Colomer,M Quintela-Fandino

American journal of physiology. Renal physiology 320:F97-F113 PubMed33308016

2020

Role of γ-adducin in actin cytoskeleton rearrangements in podocyte pathophysiology.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Gao,Yedan Liu,Letao Fan,Baoying Zheng,Joshua R Jefferson,Shaoxun Wang,Huawei Zhang,Xing Fang,Bond V Nguyen,Tongyu Zhu,Richard J Roman,Fan Fan

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ARPC1B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC1B antibody (AB227082)

Western blot - Anti-ARPC1B antibody (AB227082)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise